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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PFN1 / Profilin 1

profilin 1

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Gene Name: profilin 1
Synonyms: PFN1, ALS18, Profilin-1, Profilin 1, Profilin I
Target Sequences: NM_005022 NP_005013.1 P07737

Publications (5)

1
Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro. Salazar R, Bell SE, Davis GE. Experimental cell research. 1999 249:22-32. [PubMed:10328950]
2
Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice. Hauser W, Knobeloch KP, Eigenthaler M, Gambaryan S, Krenn V, Geiger J, Glazova M, Rohde E, Horak I, Walter U, Zimmer M. Proceedings of the National Academy of Sciences of the United States of America. 1999 96:8120-5. [PubMed:10393958] [PMC:PMC22198]
3
A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems. Giesemann T, Rathke-Hartlieb S, Rothkegel M, Bartsch JW, Buchmeier S, Jockusch BM, Jockusch H. The Journal of biological chemistry. 1999 274:37908-14. [PubMed:10608857]
4
Endothelin-1 mobilizes profilin-1-bound PIP2 in cardiac muscle. Evans NJ, Walker JW. Experimental biology and medicine (Maywood, N.J.). 2006 231:882-7. [PubMed:16741017]
5
The human submandibular gland: immunohistochemical analysis of SNAREs and cytoskeletal proteins. Stoeckelhuber M, Scherer EQ, Janssen KP, Slotta-Huspenina J, Loeffelbein DJ, Rohleder NH, Nieberler M, Hasler R, Kesting MR. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2012 60:110-20. [PubMed:22131313] [PMC:PMC3351120]
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Filtered By: None
Target
PFN1 / Profilin 1 (51)
Reactivity
Human (37)
Mouse (24)
Rat (19)
Bovine (7)
Gibbon (4)
Guinea pig (4)
Horse (4)
Mercurialis annua (4)
Monkey (4)
Pig (5)
Application
IHC (37)
IHC-P (13)
WB (47)
Flo (4)
ELISA (14)
ICC (1)
IF (2)
Peptide-ELISA (1)
Host
rabbit (49)
mouse (2)
Isotype
Ig (1)
IgG (37)
IgG1 (2)
IgG2a (1)
Clonality
monoclonal mc (2)
polyclonal pc (49)
Clone
1D5 (1)
2D2 (1)
Format
APC (2)
APC Conjugated (2)
Biotin Conjugated (7)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (6)
HRP Conjugated (5)
PE Conjugated (2)
Unconjugated (22)
Epitope
aa1-139 (10)
aa2-140 (8)
C-Terminus (6)
N-Terminus (4)
aa116-140 (1)
aa87-120 (1)
Publications
No (51)
Showing 1-25 of 51 results

PFN1 / Profilin 1 Antibody (C-Terminus) IHC-plus™ LS-B11689
Rabbit Polyclonal (IgG1) to Rat PFN1 / Profilin 1
Mouse, Bovine, Rat, Human
IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $425.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

PFN1 / Profilin 1 Antibody IHC-plus™ LS-B14410
Rabbit Polyclonal to Human PFN1 / Profilin 1
Human
Flo IHC IHC-P WB
Unconjugated
50µg  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
PFN1 Antibody Western blot of MDA-MB435,Jurkat,HeLa cell line lysates (15 ug/lane). This demonstrates the PFN1 antibody detected the PFN1 protein (arrow).
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
PFN1 Antibody Western blot of MDA-MB435,Jurkat,HeLa cell line lysates (15 ug/lane). This demonstrates the PFN1 antibody detected the PFN1 protein (arrow).

PFN1 / Profilin 1 Antibody (clone 2D2) LS-C115262
Mouse Monoclonal [clone 2D2] (IgG1) to Human PFN1 / Profilin 1
Flo IF IHC IHC-P WB
Unconjugated
100µl  $345.00
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).

PFN1 / Profilin 1 Antibody (clone 1D5) LS-C115238
Mouse Monoclonal [clone 1D5] (IgG2a) to Human PFN1 / Profilin 1
Flo IF IHC IHC-P WB
Unconjugated
100µl  $335.00
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. (Dilution 1:50).

PFN1 / Profilin 1 Antibody (N-Terminus) IHC-plus™ LS-B12715
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Human, Monkey
IHC IHC-P WB
Unconjugated
50µg  $485.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (N-Terminus, HRP) LS-C450980
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Gibbon, Human, Monkey
IHC IHC-P WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (N-Terminus, Biotin) LS-C450979
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Gibbon, Human, Monkey
IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (N-Terminus, FITC) LS-C450981
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Gibbon, Human, Monkey
IHC IHC-P WB
FITC Conjugated
100µl 1mg/ml  $385.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody LS-C345074
Rabbit Polyclonal to Human PFN1 / Profilin 1
Mouse, Bovine, Rat, Pig, Human
ICC IHC WB
Unconjugated
10µg  $405.00

PFN1 / Profilin 1 Antibody LS-C757599
Rabbit Polyclonal (Ig) to Human PFN1 / Profilin 1
Mouse, Rat, Human
ELISA Flo WB
Unconjugated
50µl  $215.00; 200µl  $325.00
All lanes: Anti-Profilin-1 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: 293 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with Profilin-1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Profilin-1 Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes: Anti-Profilin-1 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: 293 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with Profilin-1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Profilin-1 Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

PFN1 / Profilin 1 Antibody LS-C753085
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Human
ELISA IHC WB
Unconjugated
100µg  $335.00

PFN1 / Profilin 1 Antibody LS-C211761
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Human
ELISA IHC WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (HRP) LS-C211762
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Human
ELISA IHC WB
HRP Conjugated
50µg  $285.00; 100µg  $385.00
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (Biotin) LS-C211764
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Human
ELISA IHC WB
Biotin Conjugated
50µg  $285.00; 100µg  $385.00
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (FITC) LS-C211763
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Human
ELISA IHC WB
FITC Conjugated
50µg  $285.00; 100µg  $385.00
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Profilin-1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 15.4 kDa. Observed band size: 15.4 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (aa2-140) LS-C296043
Rabbit Polyclonal (IgG) to Mouse PFN1 / Profilin 1
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00; 10ml 0.5mg/ml  $2,160.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (aa2-140, HRP) LS-C711228
Rabbit Polyclonal (IgG) to Mouse PFN1 / Profilin 1
Mouse
IHC WB
HRP Conjugated
20µl  $215.00; 100µl  $295.00; 200µl  $345.00; 1ml  $695.00; 10ml  $2,805.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (aa2-140, Biotin) LS-C299969
Rabbit Polyclonal (IgG) to Mouse PFN1 / Profilin 1
Mouse
IHC WB
Biotin Conjugated
100µl 0.5mg/ml  $435.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (aa2-140, FITC) LS-C302538
Rabbit Polyclonal (IgG) to Mouse PFN1 / Profilin 1
Mouse
IHC WB
FITC Conjugated
100µl 0.5mg/ml  $465.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (aa2-140, PE) LS-C725291
Rabbit Polyclonal (IgG) to Mouse PFN1 / Profilin 1
Mouse
IHC WB
PE Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $335.00; 1ml  $650.00; 10ml  $2,580.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (aa2-140, APC) LS-C740430
Rabbit Polyclonal (IgG) to Mouse PFN1 / Profilin 1
Mouse
IHC WB
APC Conjugated
20µl  $225.00; 100µl  $315.00; 200µl  $375.00; 1ml  $815.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody (C-Terminus) LS-C142709
Rabbit Polyclonal to Human PFN1 / Profilin 1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl  $335.00
Profilin 1 Antibody - IHC of Profilin in mouse thymus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Profilin Antibody - Western Blot on HeLa whole cell extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Profilin 1 Antibody - IHC of Profilin in mouse thymus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Profilin Antibody - Western Blot on HeLa whole cell extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PFN1 / Profilin 1 Antibody LS-C192418
Rabbit Polyclonal to Human PFN1 / Profilin 1
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $385.00
Western blot of PFN1 pAb in extracts from COS7, HT29, PC12 and Jurkat cells.
Western blot of PFN1 pAb in extracts from COS7, HT29, PC12 and Jurkat cells.

PFN1 / Profilin 1 Antibody LS-C331312
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cell lines, using PFN1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using PFN1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

PFN1 / Profilin 1 Antibody (aa1-139) LS-C296041
Rabbit Polyclonal (IgG) to Human PFN1 / Profilin 1
Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PFN1 / Profilin 1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 51 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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