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PDI / P4HB

prolyl 4-hydroxylase, beta polypeptide

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

Gene Name: prolyl 4-hydroxylase, beta polypeptide
Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI
Target Sequences: NM_000918 NP_000909.2 P07237

Publications (4)

1
Proteomic analysis of solid pseudopapillary tumor of the pancreas reveals dysfunction of the endoplasmic reticulum protein processing pathway. Zhu Y, Xu H, Chen H, Xie J, Shi M, Shen B, Deng X, Liu C, Zhan X, Peng C. Molecular & cellular proteomics : MCP. 2014 13:2593-603. (WB; Human, Rat) [Full Text Article] [PubMed:24997997] [PMC:PMC4188989]
2
Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures. Wong K, Sun F, Trudel G, Sebastiani P, Laneuville O. BMC musculoskeletal disorders. 2015 16:125. (ICC; Human, Rat) [Full Text Article] [PubMed:26006773] [PMC:PMC4443538] Related Antibodies: LS-B3137.
3
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Ohe R, Aung NY, Meng H, Kabasawa T, Suto A, Tamazawa N, Yang S, Kato T, Yamakawa M. Leukemia & lymphoma. 2015 57:1687-96. (IHC; Human) [Full Text Article] [PubMed:26700650] [PMC:PMC4926777]
4
Patterns of production of collagen-rich deposits in peripheral nerves in response to injury: A pilot study in a rabbit model. Michael Rivlin, Andrew Miller, Jacob Tulipan, Pedro K Beredjiklian, Mark L Wang, Jolanta Fertala, Andrzej Steplewski, James Kostas, Andrzej Fertala. Brain and behavior. 2017 July;7:e00659. [Full Text Article] [PubMed:28729925] [PMC:PMC5516593] Related Antibodies: LS-C827808.
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Proteins (17)
Over-Expression Lysate (2)
Recombinant (15)
PDI / P4HB (17)
No (17)
6His, C-terminus (2)
6His, N-terminus (2)
GST (2)
GST, N-terminus (1)
His (6)
His-GST (1)
Myc-DDK (Flag) (3)
Hamster-Chinese (2)
Human (13)
Mouse (1)
293T Cells (1)
Bacteria (1)
E. coli (8)
HEK 293 Cells (2)
Human Cells (2)
Wheat Germ Extract (1)
Yeast (2)
Purified (5)
Low endotoxin level (4)
PDI / P4HB Protein - Purified recombinant protein P4HB was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
55.2 kDa
20 µg/$1,107
PDI / P4HB Protein
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E. coli
His-GST
60.8 kDa
10 µg/$279; 50 µg/$432; 100 µg/$690; 200 µg/$862; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148
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Bacteria
GST, N-terminus
85 kDa
100 µg/$350
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E. coli
His
59.4 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
PDI / P4HB Protein - 12.5% SDS-PAGE of human P4HB stained with Coomassie Blue
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Wheat Germ Extract
GST
83.5 kDa
10 µg/$479; 25 µg/$670
PDI / P4HB Protein
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Yeast
His
57.3 kD
1 mg/$1,607; 100 µg/$469; 20 µg/$349
Select
E. coli
GST
59.1 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
PDI / P4HB Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
PDI / P4HB Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
1 mg/$1,973; 100 µg/$679; 20 µg/$429
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293T Cells
Myc-DDK (Flag)
57.12 kDa
20 µg/$150
PDI / P4HB Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
57.12 kDa
100 µg/$494
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E. coli
His
56.6 kDa
100 µg/$469; 500 µg/$951
PDI / P4HB Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
55.9 kDa
10 µg/$368; 50 µg/$479
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E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
55.9kD
10 µg/$281; 50 µg/$395; 500 µg/$1,585; 1 mg/$2,157
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Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
56.2 kDa
100 µg/$843
Select
Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
56.4 kDa
100 µg/$721
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).