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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAX6

paired box 6

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.

Gene Name: paired box 6
Synonyms: PAX6, AN2, D11S812E, MGDA, Oculorhombin, Paired box 6, Paired box homeotic gene-6, AN, Aniridia type II protein, WAGR, Paired box protein Pax-6
Target Sequences: NM_001604 NP_001595.2 P26367

Filtered by: None
Target
PAX6 (102)
Reactivity
Human (84)
Mouse (39)
Rat (48)
Bat (3)
Bird (1)
Bovine (12)
Chicken (23)
Dog (9)
Fish (1)
Gibbon (6)
Guinea pig (5)
Hamster (3)
Horse (10)
Monkey (9)
Pig (5)
Rabbit (15)
Sheep (3)
Xenopus (5)
Zebrafish (9)
Application
IHC (39)
IHC-Fr (2)
IHC-P (17)
WB (93)
Flo (12)
ELISA (44)
ICC (24)
IF (33)
IP (1)
Peptide-ELISA (3)
Host
rabbit (75)
mouse (26)
goat (1)
Isotype
IgG (44)
IgG1 (8)
IgG1,k (9)
IgM,k (9)
Clonality
monoclonal mc (26)
polyclonal pc (76)
Clone
193CT15.2.2 (7)
1C8 (2)
1F6G2 (1)
PAX5I326 (2)
PAX6/1166 (3)
PAX6/496 (2)
PBPX6 (2)
SPM612 (2)
Format
AP Conjugated (5)
APC (1)
APC Conjugated (6)
Azide-free (8)
Biotin Conjugated (9)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (9)
HRP Conjugated (9)
PE Conjugated (6)
Ready-to-Use (1)
Unconjugated (56)
Epitope
aa1-130 (8)
aa183-210 (8)
aa352-380 (8)
aa1-300 (7)
C-Terminus (5)
N-Terminus (4)
aa32-81 (4)
aa404-436 (2)
Internal (1)
aa175-225 (1)
aa183-214 (1)
aa195-233 (1)
aa267-280 (1)
Publications
No (102)
Showing 1-25 of 102 results

IHC-plus™ PAX6 Antibody (aa32-81) LS-B991
Rabbit Polyclonal to Human PAX6
Chicken, Bovine, Rabbit, Dog, Rat, Horse, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Anti-PAX6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PAX6 Antibody LS-C179903
Rabbit Polyclonal to Mouse PAX6
Chicken, Rat, Mouse
IHC, IHC-P, WB
Unconjugated
100µl  $365.00
Immunohistochemistry Image
Immunohistochemistry Image

PAX6 Antibody LS-C109502
Rabbit Polyclonal to Human PAX6
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
100µl 1.8mg/ml  $355.00
Immunofluorescence of paraformaldehyde-fixed A549, using PAX6 antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate). A: A431. B: Hep G2. 10% SDS PAGE. PAX6 antibody diluted at 1:1000.

PAX6 Antibody (aa183-210) LS-C163394
Rabbit Polyclonal to Human PAX6
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of PAX6 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

PAX6 Antibody LS-C9501
Rabbit Polyclonal (IgG) to Human PAX6
Xenopus, Bird, Chicken, Zebrafish, Bat, Sheep, Bovine, Rabbit, Mouse, Rat, Hamster, Human
ELISA
Unconjugated
50µg  $345.00; 100µg  $465.00

PAX6 Antibody (clone PAX6/496) LS-C358010
Mouse Monoclonal [clone PAX6/496] (IgG1,k) to Human PAX6
Chicken, Mouse, Rat, Human
Flo, IF, IHC, IHC-Fr, WB
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
PAX6 antibody PAX6/496 western blot.  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (clone PAX6/496, Azide-free) LS-C389659
Mouse Monoclonal [clone PAX6/496] (IgG1,k) to Human PAX6
Chicken, Mouse, Rat, Human
Flo, IF, IHC, IHC-Fr, WB
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
PAX6 antibody PAX6/496 western blot.  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (clone PAX5I326) LS-C342315
Mouse Monoclonal [clone PAX5I326] (IgG1) to Human PAX6
Human
ELISA, ICC, IF, WB
Unconjugated
100µg 100µg/ml  $415.00
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX6 mAb.
Western blot analysis of immunized recombinant protein, using anti-PAX6 monoclonal antibody.

PAX6 Antibody LS-C354944
Rabbit Polyclonal (IgG) to Human PAX6
Mouse, Rat, Human
Flo, IF, IHC, WB
Unconjugated
100µg  $395.00
Immunohistochemistry analysis of PAX6 showing staining in the nucleus of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PAX6 Polyclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunofluorescence analysis of PAX6 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PAX6 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

PAX6 Antibody (aa267-280) LS-C55240
Goat Polyclonal to Human PAX6
Xenopus, Chicken, Zebrafish, Horse, Bat, Sheep, Bovine, Rabbit, Mouse, Rat, Hamster, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Antibody (0.5 ug/ml) staining of Mouse Eye lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PAX6 Antibody (aa352-380) LS-C163395
Rabbit Polyclonal to Human PAX6
Human
IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
PAX6-T373 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Confocal immunofluorescence of PAX6-T373 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

PAX6 Antibody (aa1-300, clone PAX6/1166) LS-C390806
Mouse Monoclonal [clone PAX6/1166] (IgG1,k) to Human PAX6
Human
Flo, IF, IHC, IHC-P
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Formalin-fixed, paraffin-embedded human gastric carcinoma stained with PAX6 antibody (PAX6/1166).  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (aa1-300, clone PAX6/1166, Azide-free) LS-C390808
Mouse Monoclonal [clone PAX6/1166] (IgG1,k) to Human PAX6
Human
Flo, IF, IHC, IHC-P
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
Formalin-fixed, paraffin-embedded human gastric carcinoma stained with PAX6 antibody (PAX6/1166).  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (aa1-300, clone PAX6/1166, Ready-to-Use) LS-C390817
Mouse Monoclonal [clone PAX6/1166] (IgG1,k) to Human PAX6
Human
Flo, IF, IHC, IHC-P
Unconjugated, Ready-to-Use
7ml  $465.00
Formalin-fixed, paraffin-embedded human gastric carcinoma stained with PAX6 antibody (PAX6/1166).  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (aa1-300, clone SPM612) LS-C390813
Mouse Monoclonal [clone SPM612] (IgG1,k) to Human PAX6
Human
Flo, IF, IHC, IHC-P
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Formalin-fixed, paraffin-embedded human gastric carcinoma stained with PAX-6 antibody (SPM612).  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (aa1-300, clone SPM612, Azide-free) LS-C390815
Mouse Monoclonal [clone SPM612] (IgG1,k) to Human PAX6
Human
Flo, IF, IHC, IHC-P
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
Formalin-fixed, paraffin-embedded human gastric carcinoma stained with PAX-6 antibody (SPM612).  This image was taken for the unmodified form of this product. Other forms have not been tested.

PAX6 Antibody (Biotin) LS-C265682
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
Biotin Conjugated
200µl  $740.00

PAX6 Antibody (Azide-free, HRP) LS-C265685
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

PAX6 Antibody (APC) LS-C265681
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
APC Conjugated
200µl  $740.00

PAX6 Antibody (aa352-380, Biotin) LS-C265688
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
Biotin Conjugated
200µl  $740.00

PAX6 Antibody (aa352-380, FITC) LS-C265690
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
FITC Conjugated
200µl  $740.00

PAX6 Antibody (aa352-380, AP) LS-C265687
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
AP Conjugated
200µl  $740.00

PAX6 Antibody (aa352-380, APC) LS-C265689
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
APC Conjugated
200µl  $740.00

PAX6 Antibody (aa352-380, Azide-free, HRP) LS-C284487
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

PAX6 Antibody (aa352-380, PE) LS-C284491
Rabbit Polyclonal (IgG) to Human PAX6
Human
ELISA, ICC, IF, IHC, WB
PE Conjugated
200µl  $740.00
Showing 1-25 of 102 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).