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PALLD / Palladin

palladin, cytoskeletal associated protein

Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.

Gene Name: palladin, cytoskeletal associated protein
Synonyms: PALLD, Myoneurin, Sarcoma antigen NY-SAR-77, SIH002, MYN, Palladin, PNCA1, CGI-151, CGI151, KIAA0992
Target Sequences: NM_016081 NP_057165.3 Q8WX93

Publications (8)

1
Characterization of palladin, a novel protein localized to stress fibers and cell adhesions. Parast MM, Otey CA. The Journal of cell biology. 2000 150:643-56. [PubMed:10931874] [PMC:PMC2175193]
2
Identification of palladin isoforms and characterization of an isoform-specific interaction between Lasp-1 and palladin. Rachlin AS, Otey CA. Journal of cell science. 2006 119:995-1004. [PubMed:16492705]
3
Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. Goicoechea S, Arneman D, Disanza A, Garcia-Mata R, Scita G, Otey CA. Journal of cell science. 2006 119:3316-24. [PubMed:16868024]
4
Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain. Wall ME, Rachlin A, Otey CA, Loboa EG. American journal of physiology. Cell physiology. 2007 293:C1532-8. [PubMed:17687002]
5
Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells. Endlich N, Otey CA, Kriz W, Endlich K. Cell motility and the cytoskeleton. 2007 64:966-76. [PubMed:17868136]
6
Palladin contributes to invasive motility in human breast cancer cells. Goicoechea SM, Bednarski B, Garca-Mata R, Prentice-Dunn H, Kim HJ, Otey CA. Oncogene. 2009 28:587-98. [PubMed:18978809] [PMC:PMC2633435]
7
Isoform-specific upregulation of palladin in human and murine pancreas tumors. Goicoechea SM, Bednarski B, Stack C, Cowan DW, Volmar K, Thorne L, Cukierman E, Rustgi AK, Brentnall T, Hwang RF, McCulloch CA, Yeh JJ, Bentrem DJ, Hochwald SN, Hingorani SR, Kim HJ, Otey CA. PloS one. 2010 5:e10347. [PubMed:20436683] [PMC:PMC2859948]
8
Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion. Brentnall TA, Lai LA, Coleman J, Bronner MP, Pan S, Chen R. PloS one. 2012 7:e30219. [PubMed:22291919] [PMC:PMC3264580]
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☰ Filters
Antibodies (38)
Proteins (6)
ELISA Kits (1)
Over-Expression Lysate (4)
Primary (38)
Recombinant (2)
Sandwich (1)
PALLD / Palladin (45)
Human (39)
Mouse (11)
Rat (8)
Bat (1)
Chicken (6)
Dog (1)
Hamster (1)
Horse (2)
Monkey (1)
Rabbit (1)
Xenopus (1)
IHC (8)
IHC-P (6)
WB (36)
Flo (1)
ELISA (3)
ICC (1)
IF (6)
IP (9)
Peptide-ELISA (3)
rabbit (30)
mouse (5)
goat (2)
sheep (1)
Product Group
IHCPlus (1)
IgG (7)
IgG1 (5)
monoclonal mc (5)
polyclonal pc (33)
1E6 (5)
96-Well Strip Plate (1)
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Atto 390 Conjugated (1)
Atto 488 Conjugated (1)
Atto 594 Conjugated (1)
Biotin Conjugated (4)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (3)
HRP Conjugated (2)
PE Conjugated (1)
PerCP Conjugated (1)
RPE Conjugated (1)
Unconjugated (15)
aa1137-1383 (8)
N-Terminus (4)
450-530 aa, Internal (1)
Internal (1)
aa1119-1131 (1)
aa1154-1304 (1)
aa13-25 (1)
aa75-125 (1)
No (45)
Tissue Homogenates (1)
GST, N-terminus (1)
His-T7 (1)
Myc-DDK (Flag) (4)
Human (4)
293T Cells (2)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
PALLD / Palladin Antibody - PALLD antibody (0.1 ug/ml) staining of Human Umbilical Cord lysate (35 ug protein/ml in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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PALLD / Palladin Goat anti-Human Polyclonal (aa1119-1131) Antibody
Xenopus, Chicken, Rabbit, Mouse, Dog, Bat, Rat, Hamster, Horse, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100 µg/$503
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PALLD / Palladin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100 µg/$341
PALLD / Palladin Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PALLD / Palladin Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
PALLD / Palladin Antibody - Palladin Antibody (1E6) - Immunofluorescence on a7r5 cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PALLD / Palladin Mouse anti-Chicken Monoclonal (1E6) Antibody
Chicken, Mouse, Rat, Human
IF, IHC, IHC-P, IP, WB
Unconjugated
0.1 ml/$386
PALLD / Palladin Antibody - Palladin Antibody (1E6) - Immunofluorescence on a7r5 cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PALLD / Palladin Mouse anti-Chicken Monoclonal (Biotin) (1E6) Antibody
Chicken, Mouse, Rat, Human
IF, IHC, IHC-P, IP, WB
Biotin Conjugated
0.1 ml/$416
PALLD / Palladin Antibody - Palladin Antibody (1E6) - Immunofluorescence on a7r5 cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PALLD / Palladin Mouse anti-Chicken Monoclonal (DY550) (1E6) Antibody
Chicken, Mouse, Rat, Human
IF, IHC, IHC-P, IP, WB
DY550 Conjugated
0.1 ml/$416
PALLD / Palladin Antibody - Palladin Antibody (1E6) - Immunofluorescence on a7r5 cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PALLD / Palladin Mouse anti-Chicken Monoclonal (DY488) (1E6) Antibody
Chicken, Mouse, Rat, Human
IF, IHC, IHC-P, IP, WB
DY488 Conjugated
0.1 ml/$416
PALLD / Palladin Antibody - Palladin Antibody (1E6) - Immunofluorescence on a7r5 cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PALLD / Palladin Mouse anti-Chicken Monoclonal (DY650) (1E6) Antibody
Chicken, Mouse, Rat, Human
IF, IHC, IHC-P, IP, WB
DY650 Conjugated
0.1 ml/$416
PALLD / Palladin ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Tissue Homogenates
1 Plate/$798
PALLD / Palladin Antibody - Western blot of PALLD / Palladin antibody.
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PALLD / Palladin Rabbit anti-Human Polyclonal (aa1137-1383) Antibody
Human
ICC, IP, WB
Unconjugated
100 µl/$303; 200 µl/$343; 500 µl/$476; 1 ml/$543; 2 ml/$715; 5 ml/$1,182
PALLD / Palladin Antibody - Western blot of PALLD / Palladin antibody.
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PALLD / Palladin Rabbit anti-Human Polyclonal (aa1137-1383) (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$341; 200 µl/$397; 500 µl/$583; 1 ml/$715; 2 ml/$953; 5 ml/$1,628
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PALLD / Palladin Rabbit anti-Human Polyclonal (450-530 aa, Internal) Antibody
Mouse, Human
ELISA, IHC
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
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PALLD / Palladin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$351
PALLD / Palladin Antibody - Detection of human and mouse Palladin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Palladin antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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PALLD / Palladin Rabbit anti-Human Polyclonal (aa75-125) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
PALLD / Palladin Antibody - Western blot analysis of PALLD using HepG2 whole cells lysates
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PALLD / Palladin Rabbit anti-Human Polyclonal Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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PALLD / Palladin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
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PALLD / Palladin Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$469
Select
PALLD / Palladin Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$469
Select
PALLD / Palladin Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$469
PALLD / Palladin Antibody - Western blot analysis of extracts of various cell lines, using PALLD antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
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PALLD / Palladin Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PALLD / Palladin Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody  at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.
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PALLD / Palladin Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µg/$442
PALLD / Palladin Antibody - Western blot of PALLD antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: PC12 whole cell lysate.
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PALLD / Palladin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
50 µl/$321; 100 µl/$403
Select
PALLD / Palladin Sheep anti-Human Polyclonal (aa1154-1304) Antibody
Human
WB
Unconjugated
100 µg/$930
Select
PALLD / Palladin Goat anti-Human Polyclonal (aa13-25) Antibody
Horse, Human
Peptide-ELISA
Unconjugated
100 µg/$401
PALLD / Palladin Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody  at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.
Select
PALLD / Palladin Rabbit anti-Human Polyclonal (Atto 390) Antibody
Human
WB
Atto 390 Conjugated
100 µg/$479
Viewing 1-25 of 45 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).