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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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p58 / PSMD3

proteasome (prosome, macropain) 26S subunit, non-ATPase, 3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count.

Gene Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Family/Subfamily: Protease Regulatory , not assigned-Protease Regulatory
Synonyms: PSMD3, p58, TSTA2, Proteasome subunit p58, RPN3, S3
Target Sequences: NM_002809 NP_002800.2 O43242

Filtered By: None
Target
p58 / PSMD3 (21)
Reactivity
Human (21)
Mouse (19)
Rat (15)
Bat (4)
Bovine (10)
Chicken (4)
Chimpanzee (6)
Dog (10)
Gibbon (6)
Guinea pig (9)
Horse (9)
Monkey (10)
Pig (8)
Rabbit (5)
Xenopus (4)
Application
IHC (8)
WB (21)
Flo (3)
ELISA (7)
IF (1)
IP (1)
Host
rabbit (19)
mouse (2)
Isotype
Ig (1)
IgG (8)
IgG2a (2)
Clonality
monoclonal mc (2)
polyclonal pc (19)
Clone
2A2 (1)
2C2 (1)
Format
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (15)
Epitope
C-Terminus (5)
aa107-156 (4)
320-400 aa, Internal (1)
Internal (1)
aa1-50 (1)
aa400-445 (1)
Publications
No (21)
Showing 1-21 of 21 results

p58 / PSMD3 Antibody (clone 2C2) LS-C173748
Mouse Monoclonal [clone 2C2] (IgG2a) to Human p58 / PSMD3
Human
Flo IF WB
Unconjugated
100µl 1mg/ml  $335.00
Anti-PSMD3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMD3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3.
Anti-PSMD3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMD3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3.

p58 / PSMD3 Antibody (clone 2A2) LS-C173767
Mouse Monoclonal [clone 2A2] (IgG2a) to Human p58 / PSMD3
Mouse, Dog, Rat, Human, Monkey
Flo WB
Unconjugated
100µl 0.95mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

p58 / PSMD3 Antibody LS-C400992
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human brain using PSMD3 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human thyroid cancer using PSMD3 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human brain using PSMD3 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human thyroid cancer using PSMD3 Polyclonal Antibody at dilution of 1:25.

p58 / PSMD3 Antibody LS-C400997
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human liver cancer using PSMD3 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human ovarian cancer using PSMD3 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human liver cancer using PSMD3 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human ovarian cancer using PSMD3 Polyclonal Antibody at dilution of 1:30.

p58 / PSMD3 Antibody LS-C404253
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human colon cancer using PSMD3 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human lung cancer using PSMD3 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human colon cancer using PSMD3 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of Human lung cancer using PSMD3 Polyclonal Antibody at dilution of 1:25.

p58 / PSMD3 Antibody (320-400 aa, Internal) LS-C385656
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00

p58 / PSMD3 Antibody LS-C751882
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Human
ELISA IHC WB
Unconjugated
100µg  $335.00

p58 / PSMD3 Antibody LS-C742621
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
50µg 1mg/ml  $265.00; 100µg 1mg/ml  $325.00

p58 / PSMD3 Antibody (C-Terminus) LS-C757603
Rabbit Polyclonal (Ig) to Human p58 / PSMD3
Mouse, Human
ELISA Flo WB
Unconjugated
50µl  $215.00; 200µl  $325.00
All lanes: Anti-PSMD3 Antibody (C-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing HeLa cells stained with PSMD3 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PSMD3 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes: Anti-PSMD3 Antibody (C-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing HeLa cells stained with PSMD3 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PSMD3 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

p58 / PSMD3 Antibody LS-C332248
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue.
Immunohistochemistry of paraffin-embedded rat spleen tissue.
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue.
Immunohistochemistry of paraffin-embedded rat spleen tissue.

p58 / PSMD3 Antibody (aa400-445) LS-C313897
Rabbit Polyclonal to Human p58 / PSMD3
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $345.00

p58 / PSMD3 Antibody (aa1-50) LS-C290476
Rabbit Polyclonal (IgG) to Human p58 / PSMD3
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Horse, Human
IP WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human and Mouse PSMD3 by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMD3 antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMD3 was also immunoprecipitated by rabbit anti-PSMD3 antibody BL13887, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of Human and Mouse PSMD3 by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMD3 antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMD3 was also immunoprecipitated by rabbit anti-PSMD3 antibody BL13887, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

p58 / PSMD3 Antibody (C-Terminus) LS-C116639
Rabbit Polyclonal to Human p58 / PSMD3
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
50µg  $365.00
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (C-Terminus, HRP) LS-C461826
Rabbit Polyclonal to Human p58 / PSMD3
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Chimpanzee, Gibbon, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (C-Terminus, Biotin) LS-C461824
Rabbit Polyclonal to Human p58 / PSMD3
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Chimpanzee, Gibbon, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (C-Terminus, FITC) LS-C461825
Rabbit Polyclonal to Human p58 / PSMD3
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Chimpanzee, Gibbon, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (aa107-156) LS-C111929
Rabbit Polyclonal to Human p58 / PSMD3
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
50µg  $365.00
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (aa107-156, HRP) LS-C461823
Rabbit Polyclonal to Human p58 / PSMD3
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (aa107-156, Biotin) LS-C461821
Rabbit Polyclonal to Human p58 / PSMD3
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (aa107-156, FITC) LS-C461822
Rabbit Polyclonal to Human p58 / PSMD3
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD3 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

p58 / PSMD3 Antibody (Internal) LS-C352728
Rabbit Polyclonal to Human p58 / PSMD3
Mouse, Bovine, Rat, Human, Monkey
WB
Unconjugated
100µl 1mg/ml  $245.00
Western blot analysis of PSMD3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Western blot analysis of PSMD3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Showing 1-21 of 21 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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