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P4HA2

prolyl 4-hydroxylase, alpha polypeptide II

P4HA2 is a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: prolyl 4-hydroxylase, alpha polypeptide II
Synonyms: P4HA2, 4-PH alpha 2, 4-PH alpha-2, C-P4Halpha(II), p4HA2
Target Sequences: NM_004199 NP_004190.1 O15460

Publications (1)

1
Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin. Omland SH, Wettergren EE, Mollerup S, Asplund M, Mourier T, Hansen AJ, Gniadecki R. BMC cancer. 2017 17:675. (IHC, ELISA; Human) [Full Text Article] [PubMed:28987144] [PMC:PMC5806272]

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Proteins (9)
Over-Expression Lysate (6)
Recombinant (3)
P4HA2 (9)
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GST (1)
His (1)
Myc-DDK (Flag) (7)
Human (6)
293T Cells (3)
E. coli (1)
HEK 293 Cells (4)
Wheat Germ Extract (1)
Purified (1)
P4HA2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
60.63 kDa
100 µg/$494
P4HA2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
60.63 kDa
100 µg/$710
P4HA2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
60.63 kDa
100 µg/$494
P4HA2 Protein - Recombinant Prolyl-4-Hydroxylase Alpha Polypeptide II By SDS-PAGE
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E. coli
His
39.9 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
P4HA2 Protein - 12.5% SDS-PAGE of human P4HA2 stained with Coomassie Blue
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Wheat Germ Extract
GST
87 kDa
10 µg/$479; 25 µg/$670
P4HA2 Protein - Purified recombinant protein P4HA2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
58.2 kDa
20 µg/$1,107
P4HA2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
60.63 kDa
20 µg/$150
P4HA2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
60.63 kDa
20 µg/$215
P4HA2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
60.63 kDa
20 µg/$150
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).