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NT5C2

5'-nucleotidase, cytosolic II

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.

Gene Name: 5'-nucleotidase, cytosolic II
Synonyms: NT5C2, 5'-nucleotidase, cytosolic II, 5-nucleotidase, cytosolic II, Cytosolic 5-nucleotidase II, GMP, NT5CP, Purine 5 nucleotidase, NT5B, CN-II, IMP-specific 5-NT, PNT5
Target Sequences: NM_012229 NP_036361.1 P49902

☰ Filters
Antibodies (17)
Proteins (9)
ELISA Kits (1)
Over-Expression Lysate (4)
Primary (17)
Recombinant (5)
Sandwich (1)
NT5C2 (27)
Human (17)
Mouse (4)
Rat (2)
Bovine (1)
Monkey (1)
IHC (3)
IHC-P (2)
WB (14)
ELISA (10)
IF (2)
Peptide-ELISA (1)
rabbit (16)
mouse (1)
IgG (14)
IgG2a,k (1)
monoclonal mc (1)
polyclonal pc (16)
3C1 (1)
96-Well Strip Plate (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (9)
aa5-33 (7)
aa108-362 (1)
No (27)
Cell Lysates (1)
Tissue Homogenates (1)
GST (1)
His (1)
His-T7, N-terminus (1)
Myc-DDK (Flag) (6)
Human (7)
293T Cells (2)
E. coli (2)
HEK 293 Cells (4)
Wheat Germ Extract (1)
Purified (3)
NT5C2 Antibody - Immunoperoxidase of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
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NT5C2 Mouse anti-Human Monoclonal (3C1) Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
100 µg/$423
NT5C2 Antibody - IHC of paraffin-embedded OVCA using NT5C2 antibody at 1:100 dilution.
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NT5C2 Rabbit anti-Human Polyclonal (aa108-362) Antibody
Mouse, Bovine, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$450
NT5C2 Antibody - Immunofluorescence staining of Hela cells at a dilution of 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
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NT5C2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, WB
Unconjugated
50 µg/$294; 100 µg/$360
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NT5C2 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
NT5C2 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
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NT5C2 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
NT5C2 Antibody - 1:100 staining human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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NT5C2 Rabbit anti-Human Polyclonal Antibody
Mouse, Human, Monkey
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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NT5C2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$341
Select
NT5C2 Rabbit anti-Human Polyclonal (aa5-33) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
NT5C2 Rabbit anti-Human Polyclonal (aa5-33) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
NT5C2 Rabbit anti-Human Polyclonal (aa5-33) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
NT5C2 Rabbit anti-Human Polyclonal (aa5-33) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
NT5C2 Rabbit anti-Human Polyclonal (aa5-33) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
Select
NT5C2 Rabbit anti-Human Polyclonal (aa5-33) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
NT5C2 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
100 µg/$357
NT5C2 Antibody - NT5C2 Antibody western blot of WiDr cell line lysates (35 ug/lane). The NT5C2 antibody detected the NT5C2 protein (arrow).
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NT5C2 Rabbit anti-Human Polyclonal (aa5-33) Antibody
Human
WB
Unconjugated
400 µl/$393
NT5C2 Antibody - Western blot analysis of extracts of various cell lines, using NT5C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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NT5C2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
NT5C2 Protein - Purified recombinant protein NT5C2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
64.8 kDa
20 µg/$1,107
NT5C2 Protein - Purified recombinant protein NT5C2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
64.8 kDa
20 µg/$1,107
Select
293T Cells
Myc-DDK (Flag)
64.8 kDa
20 µg/$150
NT5C2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
64.8 kDa
20 µg/$150
NT5C2 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Rat
0.313 - 20 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$893
NT5C2 Protein - 12.5% SDS-PAGE of human NT5C2 stained with Coomassie Blue
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Wheat Germ Extract
GST
87.45 kDa
10 µg/$479; 25 µg/$670
NT5C2 Protein - Recombinant 5'-Nucleotidase, Cytosolic II (NT5C2) by SDS-PAGE
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E. coli
His-T7, N-terminus
28.5kDa
10 µg/$244; 50 µg/$295; 200 µg/$422; 100 µg/$345; 1 mg/$1,200; 500 µg/$842; 5 mg/$2,040; 2 mg/$1,345
NT5C2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
64.8 kDa
100 µg/$494
Viewing 1-25 of 27 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).