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NSFL1C

NSFL1 (p97) Cofactor (p47)

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Gene Name: NSFL1 (p97) Cofactor (p47)
Synonyms: NSFL1C
Target Sequences: NM_016143 NP_057227.2 Q9UNZ2

☰ Filters
Antibodies (10)
Proteins (6)
Over-Expression Lysate (4)
Primary (10)
Recombinant (2)
NSFL1C (16)
Human (10)
Mouse (7)
Rat (3)
IHC (3)
IHC-P (4)
WB (10)
ELISA (4)
ICC (1)
IF (3)
IP (1)
Peptide-ELISA (1)
rabbit (10)
Ig (2)
IgG (7)
polyclonal pc (10)
Unconjugated (10)
Internal (1)
N-Terminus (1)
aa21-302 (1)
aa25-75 (1)
No (16)
GST (1)
Myc-DDK (Flag) (5)
Human (4)
293T Cells (2)
HEK 293 Cells (3)
Wheat Germ Extract (1)
Purified (1)
NSFL1C Antibody - Immunohistochemistry of paraffin-embedded human lung cancer at dilution 1:100
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NSFL1C Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
NSFL1C Antibody - Immunofluorescence analysis of HeLa cells.
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NSFL1C Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
NSFL1C Antibody - Immunofluorescent analysis of p47 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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NSFL1C Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
NSFL1C Antibody - IHC of paraffin-embedded differential N/T pair Lung SCC cancer xenograft using NSFL1C antibody at 1:100 dilution.
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NSFL1C Rabbit anti-Human Polyclonal (aa21-302) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$450
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NSFL1C Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
NSFL1C Antibody - NSFL1C Antibody (Center) staining NSFL1C in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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NSFL1C Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Human
ELISA, IHC-P, WB
Unconjugated
200 µl/$393
NSFL1C Antibody - NSFL1C Antibody (N-Term) staining NSFL1C in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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NSFL1C Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
ELISA, IHC-P, WB
Unconjugated
200 µl/$393
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NSFL1C Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$341
NSFL1C Antibody - Detection of human and mouse NSFL1C by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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NSFL1C Rabbit anti-Human Polyclonal (aa25-75) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
NSFL1C Antibody - Western blot analysis of extracts of HEK293 cells using NSFL1C antibody. The lane on the left was treated with blocking peptide.
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NSFL1C Rabbit anti-Human Polyclonal Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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293T Cells
Myc-DDK (Flag)
40.4 kDa
20 µg/$150
NSFL1C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
37.1 kDa
20 µg/$150
NSFL1C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
37.1 kDa
100 µg/$494
NSFL1C Protein - 12.5% SDS-PAGE of human NSFL1C stained with Coomassie Blue
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Wheat Germ Extract
GST
67 kDa
10 µg/$479; 25 µg/$670
NSFL1C Protein - Purified recombinant protein NSFL1C was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
40.4 kDa
20 µg/$1,107
NSFL1C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
40.4 kDa
100 µg/$494
Viewing 1-16 of 16 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).