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NOS3 / eNOS

nitric oxide synthase 3 (endothelial cell)

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Gene Name: nitric oxide synthase 3 (endothelial cell)
Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3
Target Sequences: NM_000603 NP_000594.2 P29474

Publications (2)

1
Restoration of perivascular adipose tissue function in diet-induced obese mice without changing bodyweight. Xia N, Weisenburger S, Koch E, Burkart M, Reifenberg G, Förstermann U, Li H. British journal of pharmacology. 2017 174:3443-3453. (WB; Mouse) [Full Text Article] [PubMed:28055105] [PMC:PMC5610154]
2
The Involvement of Sirtuin 1 Dysfunction in High-Fat Diet-Induced Vascular Dysfunction in Mice. Ning Xia, Gisela Reifenberg, Christian Schirra, Huige Li. Antioxidants (Basel, Switzerland). 2022 March;11: [Full Text Article] [PubMed:35326191] [PMC:PMC8944782]

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Proteins (11)
Over-Expression Lysate (2)
Recombinant (9)
NOS3 / eNOS (11)
No (11)
GST, N-terminus (1)
His (5)
His-T7 (2)
Myc-DDK (Flag) (3)
Bovine (1)
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Human (4)
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293T Cells (1)
E. coli (7)
HEK 293 Cells (2)
Wheat Germ Extract (1)
NOS3 / eNOS Protein - Recombinant Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
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E. coli
His
21.8 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
NOS3 / eNOS Protein - Recombinant  Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His
16.1 kDa
10 µg/$280; 50 µg/$437; 100 µg/$700; 200 µg/$872; 1 mg/$1,978; 500 µg/$1,519; 5 mg/$3,053; 2 mg/$2,164
NOS3 / eNOS Protein - Purified recombinant protein NOS3 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
133.1 kDa
20 µg/$1,107
NOS3 / eNOS Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
NOS3 / eNOS Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
133.1 kDa
20 µg/$150
NOS3 / eNOS Protein - Recombinant Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His-T7
24.1 kDa
50 µg/$482; 10 µg/$287; 200 µg/$962; 100 µg/$770; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
NOS3 / eNOS Protein - Recombinant Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His
14.3 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
NOS3 / eNOS Protein - Recombinant Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His
21.9 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
NOS3 / eNOS Protein - Recombinant Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His
28.7 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
NOS3 / eNOS Protein - Recombinant  Nitric Oxide Synthase 3, Endothelial By SDS-PAGE
Select
E. coli
His-T7
23.8 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
NOS3 / eNOS Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
133.1 kDa
100 µg/$494
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).