NLK

nemo-like kinase

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1.

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Proteins (6)

Type

Over-Expression Lysate (2)

Recombinant (4)

Target

NLK (6)

Publications

No (6)

Tag

GST (1)

GST, N-terminus (1)

His,N-terminus + GST (1)

Myc-DDK (Flag) (3)

Species

Human (5)

Source

293T Cells (1)

HEK 293 Cells (2)

Insect Cells (1)

Wheat Germ Extract (2)

Purification

Purified (2)

Low Endotoxin Level

Low endotoxin level (1)

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Human NLK Protein (Recombinant GST, N-terminus) (aa416-515) - LS-G62519

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human NLK Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G84427

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

57.08 kDa

Price:

20 µg/$150

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Human NLK Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G69358

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

58.1 kDa

Price:

20 µg/$1,107

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Human NLK Protein (Recombinant GST) (Full Length) - LS-G33874

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

82.39 kDa

Price:

10 µg/$479; 25 µg/$670

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Human NLK Protein (Recombinant His,N-terminus + GST) (Val121-Glu527) - LS-G139998

Source:

Insect Cells

Tag:

His,N-terminus + GST

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

74.1 kDa

Price:

50 µg/$707

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NLK Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G101405

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

57.08 kDa

Price:

100 µg/$494

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