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Unfortunately, the antibody (ID:155957) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



NFKB2

nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.

Gene Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms: NFKB2, DNA-binding factor KBF2, LYT10, Oncogene Lyt-10, p52, LYT-10, NF-kB2, p105, H2TF1
Target Sequences: NM_002502 NP_002493.3 Q00653

Filtered By: None
Target
nfkb2 (112)
Reactivity
Human (93)
Mouse (60)
Rat (34)
Bat (8)
Bovine (14)
Chicken (11)
Chimpanzee (6)
Dog (8)
Gibbon (9)
Guinea pig (4)
Hamster (4)
Horse (12)
Monkey (8)
Orangutan (4)
Pig (11)
Rabbit (9)
Application
IHC (53)
IHC-P (20)
WB (106)
DB (2)
ELISA (20)
GS (1)
ICC (6)
IF (12)
IP (26)
Peptide-ELISA (5)
PLA (1)
Host
rabbit (102)
mouse (10)
Isotype
IgG (78)
IgG1 (3)
IgG2a (6)
IgG2b (1)
Clonality
monoclonal mc (10)
polyclonal pc (102)
Clone
444C3a (2)
R272.1.1E1 (1)
Format
AP Conjugated (3)
APC (3)
APC Conjugated (6)
Azide-free (1)
Biotin Conjugated (11)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
FITC Conjugated (12)
HRP Conjugated (10)
PE Conjugated (6)
Unconjugated (58)
Epitope
aa37-342 (10)
aa38-343 (9)
aa35-225 (8)
aa848-874 (8)
C-Terminus (5)
pSer865 (5)
pSer869 (5)
aa31-80 (4)
aa65-114 (4)
aa711-758 (4)
N-Terminus (3)
pSer872 (3)
aa1-19 (2)
aa4-19 (2)
aa810-890 (2)
Ser61 (1)
aa1-21 (1)
aa1-340 (1)
aa1-447 (1)
aa301-330 (1)
aa5-21 (1)
aa810-860 (1)
aa833-882 (1)
aa836-885 (1)
pSer866 (1)
pSer870 (1)
pSer871 (1)
Publications
No (112)
Showing 1-25 out of 112 results
Anti-NFKB2 Antibody (aa1-19) IHC-plus™ LS-B396
Anti-NFKB2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B396 dilution 1:500.

Rabbit Polyclonal to Human NFKB2
Human
ELISA IHC IHC-P WB
Unconjugated
Anti-NFKB2 Antibody (aa833-882) LS-C118193
IHC of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p100/p52 (Ab-865) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot of extracts from ovary cancer cells, using NF-kappaB p100/p52 (Ab-865) Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IHC IHC-P IP Peptide-ELISA WB
Unconjugated
Anti-NFKB2 Antibody (C-Terminus) IHC-plus™ LS-B11757
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Rabbit Polyclonal to Mouse NFKB2
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser869) LS-C117427
IHC of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot of extracts from MDA-MB-435 cells treated with TNF-alpha, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The lane on the left is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IHC IHC-P IP Peptide-ELISA WB
Unconjugated
Anti-NFKB2 Antibody (aa711-758) LS-C29758
Human Smooth Muscle.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFKB2 antibody LS-C29758 was used in IHC to stain formalin-fixed, paraffin-embedded human muscle.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

Rabbit Polyclonal (IgG) to Human NFKB2
Bovine, Horse, Human
IHC IHC-P WB
Unconjugated
Anti-NFKB2 Antibody (aa836-885) IHC-plus™ LS-B11339
Anti-NFKB2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11339 dilution 1:50.
Western blot of extracts from RAW264.7 cells, using NF-kappaB p100/p52 Antibody. The lane on the right was incubated with synthetic peptide.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
Anti-NFKB2 Antibody (C-Terminus) LS-C356224
Immunohistochemical analysis of NF-kappaB p100 (pS865) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of NF-kappaB p100 (pS865) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human NFKB2
Chicken, Bovine, Rat, Pig, Mouse, Human
ICC IF IHC IHC-P IP WB
Unconjugated
Anti-NFKB2 Antibody (C-Terminus) LS-C352571
Immunohistochemical analysis of NF-kappaB p100 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of NF-kappaB p100 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human NFKB2
Mouse, Bovine, Rat, Pig, Human
ICC IF IHC IHC-P IP WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser865) LS-C380778

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
ELISA IF IHC IP WB
Unconjugated
Anti-NFKB2 Antibody (aa810-890) LS-C384868
Western blot of NFkappaB-p100 antibody
Western blot of NFkappaB-p100 antibody

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
ELISA IF IHC IP WB
Unconjugated
Anti-NFKB2 Antibody (aa38-343) IHC-plus™ LS-B14614
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant NFKB2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Human
IHC IHC-P WB
Unconjugated
Anti-NFKB2 Antibody (aa301-330) LS-C164502
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MOUSE NFKB2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of MOUSE NFKB2 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Rabbit Polyclonal to Mouse NFKB2
Mouse, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser869) LS-C380777

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
ELISA IHC IP WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser866) LS-C336050
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed HeLa cells.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody (aa1-340) LS-C344010
NFkB p100 antibody IHC-paraffin: Human Lung Cancer Tissue.
NFkB p100 antibody IHC-paraffin: Human Mammary Cancer Tissue.

Rabbit Polyclonal to Human NFKB2
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser865) LS-C189402

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser865) LS-C189404

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser869) LS-C189403

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser871) LS-C189406

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody LS-C334147
Western blot analysis of extracts of various cell lines.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IF IHC WB
Unconjugated
Anti-NFKB2 Antibody (phospho-Ser865) LS-C117426
IHC of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser865) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot of extracts from ovary cancer, using NF-kappaB p100/p52 (Phospho-Ser865) Antibody. The lane on the left is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human NFKB2
Mouse, Rat, Human
IHC IHC-P IP Peptide-ELISA WB
Unconjugated
Anti-NFKB2 Antibody (C-Terminus) LS-C368448
Immunofluorescent analysis of NF-kappaB p100 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of NF-kappaB p100 expression in Jurkat (A); MCF7 (B); HeLa (C) whole cell lysates.

Rabbit Polyclonal to Human NFKB2
Mouse, Rat, Pig, Human
ICC IF WB
Unconjugated
Anti-NFKB2 Antibody (aa1-19) LS-C126016

Rabbit Polyclonal (IgG) to Human NFKB2
Human
ELISA GS IP WB
Unconjugated
Anti-NFKB2 Antibody (aa4-19) LS-C91084

Rabbit Polyclonal to Human NFKB2
Human
ELISA ICC WB
Unconjugated
Anti-NFKB2 Antibody (aa5-21) LS-C313084
NFKB2 antibody. IHC(P): Rat Intestine Tissue.
WB of NFKB2 antibody. Lane 1: Mouse Liver Tissue Lysate. Lane 2: HEPA Cell Lysate.

Rabbit Polyclonal to Mouse NFKB2
Mouse, Rat
IHC IHC-P WB
Unconjugated

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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