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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Unfortunately, the antibody (ID:12154) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



NF-L / NEFL

neurofilament, light polypeptide

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Gene Name: neurofilament, light polypeptide
Synonyms: NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L
Target Sequences: NM_006158 NP_006149.2 P07196

Publications (2)

1
Neuron-glia interactions underlie ALS-like axonal cytoskeletal pathology. King AE, Dickson TC, Blizzard CA, Woodhouse A, Foster SS, Chung RS, Vickers JC. Neurobiology of aging. 2011 32:459-69. (ICC, IHC; Mouse) [PubMed:19427060]
2
NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons. Yokoyama K, Tezuka T, Kotani M, Nakazawa T, Hoshina N, Shimoda Y, Kakuta S, Sudo K, Watanabe K, Iwakura Y, Yamamoto T. The EMBO journal. 2011 30:4739-54. [PubMed:21946561] [PMC:PMC3243607]

Filtered By: None
Target
nf-l-nefl (75)
Reactivity
Human (64)
Mouse (44)
Rat (39)
Avian (10)
Bovine (17)
Chicken (3)
Hamster (1)
Mammal (2)
Monkey (3)
Opossum (1)
Pig (21)
Rabbit (7)
Application
IHC (55)
IHC-Fr (9)
IHC-P (17)
WB (63)
Flo (2)
ELISA (17)
ICC (6)
IF (15)
IHC-Fx (1)
IP (10)
Peptide-ELISA (2)
Host
rabbit (35)
mouse (34)
goat (1)
chicken (5)
Isotype
IgG (30)
IgG1 (19)
IgG1,k (9)
IgG2a (2)
IgG2b (2)
IgY (3)
Clonality
monoclonal mc (34)
polyclonal pc (41)
Clone
11F6 (1)
1H3 (1)
2F11 (1)
2F5 (1)
2G10 (1)
3G2 (1)
7D1 (1)
ABT-NF (1)
DA2 (3)
NF-68 (3)
Format
AP Conjugated (2)
APC (2)
APC Conjugated (4)
Biotin Conjugated (5)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (7)
HRP Conjugated (5)
Magnetic beads Conjugated (1)
PE Conjugated (4)
Sepharose Conjugated (1)
Unconjugated (42)
Epitope
aa139-234 (9)
aa281-396 (9)
C-Terminus (2)
Internal (2)
Ile344 (1)
aa295-344 (1)
aa330-343 (1)
aa485-530 (1)
Publications
No (75)
Showing 1-25 of 75 results

NF-L / NEFL Antibody IHC-plus™ LS-B257
Rabbit Polyclonal to Pig NF-L / NEFL
Avian, Pig, Mammal, Mouse, Bovine, Rat, Human
IHC IHC-Fr IHC-P
Unconjugated
50µl  $425.00
Anti-NEFL / NF-L antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B257 concentration 5 ug/ml.
Anti-NEFL / NF-L antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B257 concentration 5 ug/ml.

NF-L / NEFL Antibody LS-C16795
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IHC-Fr IHC-P IP WB
Unconjugated
200µg 0.5mg/ml  $605.00

NF-L / NEFL Antibody (HRP) LS-C548312
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IP WB
HRP Conjugated
100µl  $800.00

NF-L / NEFL Antibody (Biotin) LS-C528209
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IP WB
Biotin Conjugated
100µl  $800.00

NF-L / NEFL Antibody (FITC) LS-C538167
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IP WB
FITC Conjugated
100µl  $800.00

NF-L / NEFL Antibody (APC) LS-C518348
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IP WB
APC Conjugated
100µl  $800.00

NF-L / NEFL Antibody (PE) LS-C656333
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IP WB
PE Conjugated
100µl  $800.00

NF-L / NEFL Antibody (AP) LS-C510171
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
ELISA IHC IP WB
AP Conjugated
100µl  $800.00

NF-L / NEFL Antibody IHC-plus™ LS-B10417
Rabbit Polyclonal to Human NF-L / NEFL
Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NF-L / NEFL antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10417 dilution 1:100.
Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NF-L / NEFL antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10417 dilution 1:100.

NF-L / NEFL Antibody IHC-plus™ LS-B10376
Chicken Polyclonal (IgY) to Human NF-L / NEFL
Human
ICC IF IHC IHC-P WB
Unconjugated
50µl 21.5mg/ml  $425.00
Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10376 dilution 1:500.
View of mixed neuron/glial cultures stained with NF-L / NEFL antibody (red) and rabbit antibody to phosphorylated NF-H (green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.
Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10376 dilution 1:500.
View of mixed neuron/glial cultures stained with NF-L / NEFL antibody (red) and rabbit antibody to phosphorylated NF-H (green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.

NF-L / NEFL Antibody (clone 2F11) IHC-plus™ LS-B7182
Mouse Monoclonal [clone 2F11] (IgG1) to Human NF-L / NEFL
Opossum, Human
IHC IHC-Fr IHC-P WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-NEFL / NF-L antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7182 concentration 10 ug/ml.
Anti-NEFL / NF-L antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7182 concentration 10 ug/ml.

NF-L / NEFL Antibody (clone 3G2) LS-C175341
Mouse Monoclonal [clone 3G2] (IgG1) to Human NF-L / NEFL
Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $335.00
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NF-L / NEFL Antibody (clone 1H3) LS-C169458
Mouse Monoclonal [clone 1H3] (IgG1) to Human NF-L / NEFL
Human
ELISA Flo IF IHC WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded lung cancer tissues using NEFL mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using NEFL mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded lung cancer tissues using NEFL mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using NEFL mouse monoclonal antibody with DAB staining.

NF-L / NEFL Antibody (clone 2G10) LS-C169461
Mouse Monoclonal [clone 2G10] (IgG1) to Human NF-L / NEFL
Human
ELISA Flo IF IHC WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded lung cancer tissues using NEFL mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded brain tissues using NEFL mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded lung cancer tissues using NEFL mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded brain tissues using NEFL mouse monoclonal antibody with DAB staining.

NF-L / NEFL Antibody (aa295-344) IHC-plus™ LS-B12562
Rabbit Polyclonal (IgG) to Human NF-L / NEFL
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA
Unconjugated
100µl 1mg/ml  $505.00
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

NF-L / NEFL Antibody LS-C667153
Rabbit Polyclonal (IgG) to Human NF-L / NEFL
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $335.00

NF-L / NEFL Antibody LS-C330877
Rabbit Polyclonal (IgG) to Human NF-L / NEFL
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibodyat dilution of 1:200 (40x lens).

NF-L / NEFL Antibody LS-C124242
Mouse Monoclonal (IgG2a) to Bovine NF-L / NEFL
Mouse, Bovine, Rat, Hamster, Human, Monkey
IF IHC IHC-Fr IHC-P WB
Unconjugated
100µl  $605.00

NF-L / NEFL Antibody (clone DA2) LS-C2725
Mouse Monoclonal [clone DA2] (IgG1,k) to Pig NF-L / NEFL
Avian, Pig, Mammal, Mouse, Rat, Human
IF IHC IHC-Fx IHC-P WB
Unconjugated
50µl  $335.00
Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody LS-C2725 (lane 4).
Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody LS-C2725 (lane 4).

NF-L / NEFL Antibody (clone 7D1) LS-C204557
Mouse Monoclonal [clone 7D1] (IgG2b) to Human NF-L / NEFL
Mouse, Bovine, Rat, Pig, Human
ICC IF WB
Unconjugated
100µl  $285.00
Embryonic rat cortical cells grown in tissue culture and stained with NF-L / NEFL antibody (green) and the phosphorylated axonal form of neurofilament NF-H, using NF-H antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with NF-L / NEFL antibody but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.
Strip blots of rat spinal cord homogenate probed with two subclones of NF-L / NEFL antibody. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68 kDa with essentially no background.
Embryonic rat cortical cells grown in tissue culture and stained with NF-L / NEFL antibody (green) and the phosphorylated axonal form of neurofilament NF-H, using NF-H antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with NF-L / NEFL antibody but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.
Strip blots of rat spinal cord homogenate probed with two subclones of NF-L / NEFL antibody. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68 kDa with essentially no background.

NF-L / NEFL Antibody LS-C122209
Mouse Monoclonal (IgG1) to Pig NF-L / NEFL
Rat, Pig, Human
IHC IHC-Fr IHC-P WB
Unconjugated
100µg  $530.00

NF-L / NEFL Antibody (clone NF-68) LS-C171214
Mouse Monoclonal [clone NF-68] (IgG1) to Pig NF-L / NEFL
Rat, Pig, Human
IHC IHC-Fr IHC-P WB
Unconjugated
100µg 1ml 1mg/ml  $295.00

NF-L / NEFL Antibody LS-C682222
Rabbit Polyclonal (IgG) to Human NF-L / NEFL
Human
ELISA IF IHC
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
Immunofluorescent analysis of SH-SY5Y cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
Immunofluorescent analysis of SH-SY5Y cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NF-L / NEFL Antibody LS-C16793
Rabbit Polyclonal (IgG) to Pig NF-L / NEFL
Avian, Pig, Mouse, Bovine, Rat, Human
IHC IHC-Fr IHC-P WB
Unconjugated
50µl  $530.00

NF-L / NEFL Antibody (clone DA2) LS-C204554
Mouse Monoclonal [clone DA2] (IgG1,k) to Pig NF-L / NEFL
Mouse, Bovine, Rat, Pig, Human
ICC IF WB
Unconjugated
500µl  $285.00
Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCA-a-Int (red). These cells were mitotic but had several characteristics of neurons.
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).
Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCA-a-Int (red). These cells were mitotic but had several characteristics of neurons.
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).
Showing 1-25 of 75 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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