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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEFM / NF-M

neurofilament, medium polypeptide

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Name: neurofilament, medium polypeptide
Synonyms: NEFM, 160 kDa neurofilament protein, NEF3, NF-M, NFM, Neurofilament 3
Target Sequences: NM_005382 NP_005373.2 P07197

Publications (3)

1
Enhancement of the growth and differentiation of nasal olfactory mucosa cells by the conjugation of growth factors to functional nanoparticles. Skaat H, Ziv-Polat O, Shahar A, Margel S. Bioconjugate chemistry. 2011 22:2600-10. (ICC; Rat) [PubMed:22029397]
2
Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering. Ziv-Polat O, Skaat H, Shahar A, Margel S. International journal of nanomedicine. 2012 7:1259-74. (ICC; Rat) [PubMed:22419873] [PMC:PMC3298389]
3
Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Louis ED, Ma K, Babij R, Corts E, Liem RK, Vonsattel JP, Faust PL. Neuroscience letters. 2012 518:49-54. (IHC, WB; Human) [PubMed:22561033] [PMC:PMC3364538]

Viewing 1-25 of 88 items
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Select
Neuroscience
Mouse Monoclonal [clone RNF406] (IgG1) to Human NEFM / NF-M
Xenopus, Hamster, Rabbit, Guinea pig, Human, Monkey
Flo, IHC, IHC-Fr, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Neuroscience
Goat Polyclonal to Human NEFM / NF-M
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µg   0.5mg/ml   $425.00
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Select
Neuroscience
Mouse Monoclonal [clone RNF403] (IgG1) to Human NEFM / NF-M
Xenopus, Hamster, Human, Monkey
IHC, IHC-Fr, IHC-P, WB
Unconjugated
50µg   1mg/ml   $425.00
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal to Rat NEFM / NF-M
Avian, Pig, Cat, Mouse, Bovine, Rat, Human
IHC, IHC-Fr, IHC-P
Unconjugated
100µl   $425.00
NEFM / NF-M Antibody - Immunohistochemistry analysis of the neurofilament medium chain showing staining in the filaments of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament medium chain monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Select
Mouse Monoclonal [clone RMO 14.9] (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µg   0.5mg/ml   $395.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µg   $635.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
HRP Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
Biotin Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
FITC Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
AP Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
PE Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1) to Rat NEFM / NF-M
Chicken, Rabbit, Mouse, Rat, Human
ELISA, IF, IHC, WB
APC Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1) to Bovine NEFM / NF-M
Chicken, Bovine, Human
ELISA, Flo, ICC, IF, IHC, IHC-Fr, IHC-P, RIA, WB
Unconjugated
100µl   $680.00
Select
Chicken Polyclonal (IgY) to Mouse NEFM / NF-M
Mouse, Human
IHC
Unconjugated
100µl   $425.00
NEFM / NF-M Antibody - Section of rat cerebral cortex stained with NEFM / NF-M antibody (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrenergic receptor kinase 1.
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Rabbit Polyclonal to Human NEFM / NF-M
Human
ICC, IF, IHC, WB
Unconjugated
100µl   $285.00
Select
Rabbit Polyclonal (IgG) to Human NEFM / NF-M
Human
ELISA, IF, IHC, WB
Unconjugated
100µg   $355.00
Select
Mouse Monoclonal (IgG1,k) to Rat NEFM / NF-M
Rat, Human
ELISA, Flo, IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
100µg   $635.00
Select
Mouse Monoclonal (IgG1,k) to Rat NEFM / NF-M
Rat, Human
ELISA, Flo, IHC, IP, WB
FITC Conjugated
100µl   $815.00
Select
Mouse Monoclonal (IgG1,k) to Rat NEFM / NF-M
Rat, Human
ELISA, Flo, IHC, IP, WB
AP Conjugated
100µl   $815.00
NEFM / NF-M Antibody - IHC of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Select
Mouse Monoclonal [clone OTI2G3] (IgG1) to Human NEFM / NF-M
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
NEFM / NF-M Antibody - Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Select
Mouse Monoclonal [clone OTI2G3] (IgG1) to Human NEFM / NF-M
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
NEFM / NF-M Antibody - IHC of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Select
Mouse Monoclonal [clone OTI5B7] (IgG1) to Human NEFM / NF-M
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
NEFM / NF-M Antibody - Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Select
Mouse Monoclonal [clone OTI5B7] (IgG1) to Human NEFM / NF-M
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
NEFM / NF-M Antibody - Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.
Select
Mouse Monoclonal [clone 3H11] (IgG1,k) to Rat NEFM / NF-M
Avian, Mammal, Mouse, Rat, Human
IF, IHC, IHC-Fx, IHC-P, WB
Unconjugated
50µl   $335.00
Select
Mouse Monoclonal (IgG1) to Human NEFM / NF-M
Xenopus, Hamster, Human, Monkey
IHC, IHC-Fr, IHC-P, WB
Unconjugated
100µg   $560.00

Viewing 1-25 of 88 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).