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NDUFA5

NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain. The predicted protein is a 116 amino acid peptide with a molecular weight of 13.34 kDa. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The deduced peptide belongs to the 7 component-containing, water soluble ""iron-sulfur protein"" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal.

Gene Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5
Synonyms: NDUFA5, B13, Complex I 13kDa subunit B, CI-13KD-B, CI-13kB, Complex I subunit B13, Complex I-13kD-B, NUFM, Type I dehydrogenase, Ubiquinone reductase, UQOR13
Target Sequences: NM_005000 NP_004991.1 Q16718

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Proteins (5)
Over-Expression Lysate (2)
Recombinant (3)
NDUFA5 (5)
No (5)
GST (1)
His (1)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
NDUFA5 Protein - Purified recombinant protein NDUFA5 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
13.3 kDa
20 µg/$1,107
NDUFA5 Protein - 12.5% SDS-PAGE of human NDUFA5 stained with Coomassie Blue
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Wheat Germ Extract
GST
39.9 kDa
10 µg/$479; 25 µg/$670
NDUFA5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
13.3 kDa
100 µg/$494
NDUFA5 Protein
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E. coli
His
20 µg/$361; 100 µg/$739; 4 µg/$261
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293T Cells
Myc-DDK (Flag)
13.3 kDa
20 µg/$150
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).