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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NCS1 / Neuronal Calcium Sensor

neuronal calcium sensor 1

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Gene Name: neuronal calcium sensor 1
Synonyms: NCS1, Frequenin (Drosophila) homolog, FREQ, FLUP, Frequenin homolog, Neuronal calcium sensor 1, Frequenin homolog (Drosophila), Frequenin-like protein, NCS-1
Target Sequences: NM_014286 NP_055101.2 P62166

Filtered By: None
Target
NCS1 / Neuronal Calcium Sensor (45)
Reactivity
Human (45)
Mouse (15)
Rat (15)
Bat (4)
Bovine (8)
Chicken (9)
Chimpanzee (7)
Dog (4)
Gibbon (3)
Guinea pig (4)
Horse (4)
Monkey (5)
Orangutan (7)
Pig (4)
Xenopus (9)
Zebrafish (8)
Application
IHC (30)
IHC-P (13)
WB (45)
Flo (9)
ELISA (29)
ICC (2)
IF (5)
Host
rabbit (35)
mouse (8)
sheep (2)
Isotype
IgG (27)
IgG1,k (1)
IgG2a,k (7)
Clonality
monoclonal mc (8)
polyclonal pc (37)
Clone
AT11B8 (1)
Format
AP Conjugated (3)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (3)
Unconjugated (21)
Epitope
aa1-30 (8)
aa118-144 (8)
C-Terminus (7)
N-Terminus (4)
aa1-190 (2)
aa1-138 (1)
Publications
No (45)
Showing 1-25 of 45 results

NCS1 / Neuronal Calcium Sensor Antibody IHC-plus™ LS-B1457
Rabbit Polyclonal to Mouse NCS1 / Neuronal Calcium Sensor
Mouse, Rat, Human
ELISA IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-NCS-1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FREQUENIN Antibody - Immunofluorescence Microscopy. Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Gross et al., (2000) J. Neurosci. 20:1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numer-ous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.
Anti-NCS-1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FREQUENIN Antibody - Immunofluorescence Microscopy. Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Gross et al., (2000) J. Neurosci. 20:1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numer-ous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.

NCS1 / Neuronal Calcium Sensor Antibody (N-Terminus) LS-C30071
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Xenopus, Chicken, Zebrafish, Bovine, Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µg  $365.00
NCS1 / NCS-1 antibody ARP34315_P050-NP_055101-FREQ (frequenin homolog (Drosophila)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NCS1 / NCS-1 antibody ARP34315_P050-NP_055101-FREQ (frequenin homolog (Drosophila)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NCS1 / Neuronal Calcium Sensor Antibody (C-Terminus) IHC-plus™ LS-B10806
Rabbit Polyclonal to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Anti-NCS1 / NCS-1 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NCS1 / NCS-1 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

NCS1 / Neuronal Calcium Sensor Antibody LS-C755325
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IF IHC WB
Unconjugated
100µg  $335.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144) LS-C327404
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144, Biotin) LS-C277409
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
Biotin Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144, FITC) LS-C277410
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
FITC Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144, Azide-free, HRP) LS-C277412
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144, AP) LS-C277407
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
AP Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144, PE) LS-C277414
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
PE Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144, APC) LS-C277408
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo IHC WB
APC Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (C-Terminus) IHC-plus™ LS-B6603
Rabbit Polyclonal to Human NCS1 / Neuronal Calcium Sensor
Xenopus, Chicken, Zebrafish, Pig, Horse, Mouse, Dog, Guinea pig, Bovine, Bat, Rat, Human, Monkey
IHC IHC-P WB
Unconjugated
50µg  $485.00
Anti-NCS-1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NCS1 / NCS-1 antibody Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NCS-1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NCS1 / NCS-1 antibody Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

NCS1 / Neuronal Calcium Sensor Antibody (aa118-144) LS-C97745
Rabbit Polyclonal to Human NCS1 / Neuronal Calcium Sensor
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of NCS1 Antibody in MCF-7 cell line lysates (35 ug/lane). NCS1 (arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of NCS1 Antibody in MCF-7 cell line lysates (35 ug/lane). NCS1 (arrow) was detected using the purified antibody.

NCS1 / Neuronal Calcium Sensor Antibody (aa1-190) LS-C375734
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Mouse, Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Western blot. All lanes: Neuronal calcium sensor 1 antibody at 10 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: rat kidney tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 22 kDa. Observed band size: 22 kDa Immunohistochemistry.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Western blot. All lanes: Neuronal calcium sensor 1 antibody at 10 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: rat kidney tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 22 kDa. Observed band size: 22 kDa Immunohistochemistry.

NCS1 / Neuronal Calcium Sensor Antibody LS-C748630
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00
Immunofluorescence analysis of A549 cells using NCS1 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NCS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Immunofluorescence analysis of A549 cells using NCS1 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NCS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

NCS1 / Neuronal Calcium Sensor Antibody LS-C334734
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of A549 cells.
Western blot analysis of extracts of various cell lines.
Immunofluorescence analysis of A549 cells.
Western blot analysis of extracts of various cell lines.

NCS1 / Neuronal Calcium Sensor Antibody LS-C482619
Rabbit Polyclonal to Human NCS1 / Neuronal Calcium Sensor
Mouse, Rat, Human
ICC IF WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunofluorescent analysis of NCS1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of NCS1 expression in HeLa (A); MCF7 (B); mouse bone marrow (C); rat kidney (D) whole cell lysates.
Immunofluorescent analysis of NCS1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of NCS1 expression in HeLa (A); MCF7 (B); mouse bone marrow (C); rat kidney (D) whole cell lysates.

NCS1 / Neuronal Calcium Sensor Antibody (clone AT11B8) LS-C755515
Mouse Monoclonal [clone AT11B8] (IgG1,k) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA Flo WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $315.00
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: K562 cell lysate Lane 3.: LaCap cell lysate Lane 4.: HeLa cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: K562 cell lysate Lane 3.: LaCap cell lysate Lane 4.: HeLa cell lysate

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30) LS-C321462
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, Biotin) LS-C257392
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, FITC) LS-C257391
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, Azide-free, HRP) LS-C257394
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, PE) LS-C257395
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, APC) LS-C259430
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, AP) LS-C259428
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00
Showing 1-25 of 45 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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