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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NAMPT / Visfatin

nicotinamide phosphoribosyltransferase

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.

Gene Name: nicotinamide phosphoribosyltransferase
Synonyms: NAMPT, PBEF, Pre-B cell-enhancing factor, VISFATIN, VF, NAmPRTase, PBEF1
Target Sequences: NM_005746 NP_005737.1 P43490

Publications (1)

1
Monomeric eNAMPT in the development of experimental diabetes in mice: a potential target for type 2 diabetes treatment. Kieswich J, Sayers SR, Silvestre MF, Harwood SM, Yaqoob MM, Caton PW. Diabetologia. 2016 59:2477-2486. (WB; Mouse) [Full Text Article] [PubMed:27541013] [PMC:PMC5506101] Related Antibodies: LS-C48964.

Viewing 1-25 of 119 items
NAMPT / Visfatin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Mouse Monoclonal [clone OMNI379] (IgG2a,k) to Human NAMPT / Visfatin
Mouse, Rat, Human
ELISA, Flo, ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
50µg   1mg/ml   $425.00
NAMPT / Visfatin Antibody - Anti-NAMPT antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Chicken, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µl   $425.00
NAMPT / Visfatin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $425.00
NAMPT / Visfatin Antibody - Western blot of NAMPT / Visfatin antibody.
Select
(1) Publication(s)
Rabbit Polyclonal to Mouse NAMPT / Visfatin
Mouse, Human
WB
Unconjugated
30µg   $245.00; 100µg   200µl   $415.00
NAMPT / Visfatin Antibody - Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Select
Mouse Monoclonal [clone 4D5] (IgG1,k) to Human NAMPT / Visfatin
ELISA, WB
Unconjugated
50µl   1mg/ml   $315.00; 100µl   1mg/ml   $345.00
NAMPT / Visfatin Antibody - Western blot of NAMPT / Visfatin antibody.
Select
Rabbit Polyclonal to Rat NAMPT / Visfatin
Rat
WB
Unconjugated
30µg   $245.00; 100µg   200µl   0.5mg/ml   $385.00
NAMPT / Visfatin Antibody - NAMPT antibody ARP42255_T100-NP_005737-PBEF1(pre-B-cell colony enhancing factor 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Xenopus, Chicken, Guinea pig, Human
IHC, IHC-P, WB
Unconjugated
100µl   0.5mg/ml   $335.00
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human ovary cancer using NAMPT antibody.
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Mouse, Human
IF, IHC, WB
Unconjugated
50µl   $235.00; 100µl   $315.00; 200µl   $425.00
NAMPT / Visfatin Antibody - Western blot of NAMPT in multiple samples using Polyclonal Antibody to NAMPT/Visfatin at 1:1000. Lanes: 1. human spleen, 2. human spleen in the presence of immunizing/blocking peptide, 3. HeLa, 4. Jurkat, 5. Rh30, 6. T98G, 7. HCT-116.
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Xenopus, Chicken, Zebrafish, Horse, Mouse, Dog, Bovine, Rat, Human, Monkey
ELISA, WB
Unconjugated
100µl   $335.00
NAMPT / Visfatin Antibody - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Biotin) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone OMNI379] (IgG2a,k) to Human NAMPT / Visfatin
Mouse, Rat, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Biotin Conjugated
50µg   $355.00
NAMPT / Visfatin Antibody - Detection of Human by Immunohistochemistry. Sample: FFPE section of human placental fetal membrane. Antibody: Affinity purified rabbit anti- used at a dilution of 1:1000 (1 Detection: DAB.
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $425.00
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Human
ELISA, WB
Biotin Conjugated
200µl   $740.00
NAMPT / Visfatin Antibody - Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker; Lane 2.Reference control; Lane 3-4.Positive control, Hela and MD231 whole cell lysate; Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Human
ELISA, WB
Biotin Conjugated, Preservative Free
50µg   $295.00
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human bladder cancer at dilution of 1:100
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
NAMPT / Visfatin Antibody
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human liver using antibody at 1:100 dilution.
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl   $285.00; 100µl   $385.00
NAMPT / Visfatin Antibody - Visfatin was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- Visfatin Antigen Affinity purified polyclonal antibody
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-P, WB
Unconjugated
100µg   $325.00
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Human
ELISA, IHC, IP, WB
Unconjugated
100µg   $335.00
NAMPT / Visfatin Antibody - Western blot analysis using anti-Nampt (human), pAb at 1:2000 dilution. 1: Human Nampt (His-tagged). 2: LPS-treated human peripheral blood leukocyte lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-P, WB
Unconjugated
100µg   $365.00
NAMPT / Visfatin Antibody - Western blot analysis using anti-Nampt (human), pAb at 1:2000 dilution. 1: Human Nampt (His-tagged). 2: LPS-treated human peripheral blood leukocyte lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-P, WB
Biotin Conjugated
50µg   $365.00
NAMPT / Visfatin Antibody - Western blot analysis using anti-Nampt (human), pAb at 1:2000 dilution. 1: Human Nampt (His-tagged).
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-Fr, WB
Unconjugated
100µg   $365.00
NAMPT / Visfatin Antibody - Western blot of NAMPT / Visfatin antibody.
Select
Rabbit Polyclonal to Human NAMPT / Visfatin
Pig, Human
ICC, IP, WB
Unconjugated
20µl   0.5mg/ml   $195.00; 100µl   0.5mg/ml   $245.00; 200µl   0.5mg/ml   $285.00; 1ml   0.5mg/ml   $475.00
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human liver using antibody at 1:100 dilution.
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Human
ELISA, IHC, IHC-P
Unconjugated
50µl   $285.00; 100µl   $385.00
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded Human colon cancer using NAMPT Polyclonal Antibody at dilution of 1:30.
Select
Rabbit Polyclonal (IgG) to Human NAMPT / Visfatin
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl   0.2mg/ml   $205.00; 60µl   0.2mg/ml   $235.00; 120µl   0.2mg/ml   $295.00; 200µl   0.2mg/ml   $395.00

Viewing 1-25 of 119 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).