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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NAE1 / APPBP1

NEDD8 activating enzyme E1 subunit 1

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Gene Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: NAE1, A-116A10.1, APPBP1, HPP1, Proto-oncogene protein 1, APP-BP1, Ula-1, Protooncogene protein 1
Target Sequences: NM_003905 NP_003896.1 Q13564

Filtered by: None
Target
NAE1 / APPBP1 (57)
Reactivity
Human (57)
Mouse (10)
Rat (10)
Application
IHC (21)
IHC-P (5)
WB (56)
ELISA (27)
IP (1)
Host
rabbit (38)
mouse (19)
Isotype
IgG (20)
IgG1 (6)
IgG2a (2)
IgG2a,k (7)
IgG2b (4)
Clonality
monoclonal mc (19)
polyclonal pc (38)
Clone
4E8-H3 (1)
OTI1A10 (2)
OTI1A8 (2)
OTI1B2 (2)
OTI1D12 (2)
OTI1E10 (2)
OTI1E9 (2)
Format
AP Conjugated (3)
APC (1)
APC Conjugated (4)
Azide-free (2)
Biotin Conjugated (5)
Carrier-free (6)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (4)
Unconjugated (29)
Epitope
aa1-274 (12)
aa172-200 (8)
aa430-459 (8)
aa308-357 (4)
aa181-534 (1)
aa452-482 (1)
Publications
No (57)
Showing 1-25 of 57 results

NAE1 / APPBP1 Antibody (aa430-459) LS-C100844
Rabbit Polyclonal to Human NAE1 / APPBP1
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2) (Origene Technologies).

NAE1 / APPBP1 Antibody (aa1-274, clone OTI1E10) LS-C339158
Mouse Monoclonal [clone OTI1E10] (IgG1) to Human NAE1 / APPBP1
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

NAE1 / APPBP1 Antibody (aa1-274, clone OTI1E10, Carrier-free) LS-C790253
Mouse Monoclonal [clone OTI1E10] (IgG1) to Human NAE1 / APPBP1
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,

NAE1 / APPBP1 Antibody LS-C676116
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human pancreatic tissue at dilution 1:100
Western blot All Lanes: NAE1 antibody at 1.85ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: Mouse skeletal muscle tissue Lane 4: 293T whole cell lysate Lane 5: A431 whole cell lysate Lane 6: K562 whole cell lysate Lane 7: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61,60,51 kDa Observed band size: 60 kDa

NAE1 / APPBP1 Antibody (aa172-200) LS-C323221
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

NAE1 / APPBP1 Antibody (aa172-200, Biotin) LS-C263942
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa172-200, FITC) LS-C263943
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
FITC Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa172-200, AP) LS-C263940
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa172-200, APC) LS-C263941
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
APC Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa172-200, Azide-free, HRP) LS-C263944
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

NAE1 / APPBP1 Antibody (aa172-200, PE) LS-C263945
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
PE Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa430-459) LS-C326841
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

NAE1 / APPBP1 Antibody (aa430-459, Biotin) LS-C275101
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa430-459, FITC) LS-C275102
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
FITC Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa430-459, PE) LS-C275104
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
PE Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa430-459, APC) LS-C275100
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
APC Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa430-459, AP) LS-C275099
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

NAE1 / APPBP1 Antibody (aa430-459, Azide-free, HRP) LS-C275103
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

NAE1 / APPBP1 Antibody (aa452-482) LS-C100847
Rabbit Polyclonal to Human NAE1 / APPBP1
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human testis tissue reacted with APPBP1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of APPBP1 (arrow) using rabbit polyclonal APPBP1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APPBP1 gene (Lane 2) (Origene Technologies).

NAE1 / APPBP1 Antibody (aa172-200) LS-C164736
Rabbit Polyclonal to Human NAE1 / APPBP1
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).

NAE1 / APPBP1 Antibody LS-C660462
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Human
ELISA, IP, WB
Unconjugated
100µg  $405.00

NAE1 / APPBP1 Antibody (clone 4E8-H3) LS-C306305
Mouse Monoclonal [clone 4E8-H3] (IgG2a,k) to Human NAE1 / APPBP1
Human
ELISA, WB
Unconjugated
100µg  $560.00

NAE1 / APPBP1 Antibody LS-C401265
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human lung cancer using NAE1 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of paraffin-embedded Human lung cancer using NAE1 Polyclonal Antibody at dilution of 1:20.

NAE1 / APPBP1 Antibody LS-C778836
Rabbit Polyclonal to Human NAE1 / APPBP1
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg 100µl 1mg/ml  $305.00

NAE1 / APPBP1 Antibody LS-C404539
Rabbit Polyclonal (IgG) to Human NAE1 / APPBP1
Mouse, Rat, Human
ELISA, WB
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Western blot analysis of Human fetal muscle, kidney and liver tissue, using NAE1 Polyclonal Antibody at dilution of 1:500.
Showing 1-25 of 57 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).