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MTHFD1

methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase

MTHFD1 encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

Gene Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Synonyms: MTHFD1, C1-THF synthase, MTHFC, MTHFD
Target Sequences: NM_005956 NP_005947.3 P11586

☰ Filters
Antibodies (18)
Proteins (5)
Over-Expression Lysate (2)
Primary (18)
Recombinant (3)
MTHFD1 (23)
Human (18)
Mouse (14)
Rat (10)
Bat (5)
Chimpanzee (6)
Dog (4)
Gibbon (6)
Guinea pig (4)
Hamster (1)
Horse (5)
Monkey (9)
Orangutan (6)
Pig (9)
Rabbit (9)
Zebrafish (8)
IHC (12)
IHC-P (11)
WB (16)
ELISA (2)
ICC (2)
IF (3)
IP (4)
Peptide-ELISA (1)
rabbit (17)
goat (1)
Product Group
IHCPlus (3)
IgG (12)
polyclonal pc (18)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Preservative Free (1)
Unconjugated (12)
aa74-123 (4)
aa863-912 (4)
aa137-150 (1)
aa150-200 (1)
aa50-100 (1)
aa535-562 (1)
No (23)
6His-SUMO, N-terminus (1)
GST (1)
Myc-DDK (Flag) (3)
Human (3)
Orangutan (1)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
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MTHFD1 Goat anti-Human Polyclonal (aa137-150) Antibody
Rabbit, Mouse, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$460
MTHFD1 Antibody - MTHFD1 Antibody (Center P550) IHC of formalin-fixed and paraffin-embedded human Lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry.
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MTHFD1 Rabbit anti-Human Polyclonal (aa535-562) Antibody
Human
IF, IHC, IHC-P, WB
Unconjugated
400 µl/$376
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MTHFD1 Rabbit anti-Human Polyclonal (aa74-123) Antibody
Zebrafish, Pig, Rat, Rabbit, Mouse, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100 µl/$485
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MTHFD1 Rabbit anti-Human Polyclonal (aa74-123) (HRP) Antibody
Zebrafish, Pig, Rabbit, Mouse, Rat, Human, Monkey, Orangutan, Gibbon, Chimpanzee
IHC, IHC-P, WB
HRP Conjugated
100 µl/$459
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MTHFD1 Rabbit anti-Human Polyclonal (aa74-123) (Biotin) Antibody
Zebrafish, Pig, Rabbit, Mouse, Rat, Human, Monkey, Orangutan, Gibbon, Chimpanzee
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$459
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MTHFD1 Rabbit anti-Human Polyclonal (aa74-123) (FITC) Antibody
Zebrafish, Pig, Rabbit, Mouse, Rat, Human, Monkey, Orangutan, Gibbon, Chimpanzee
IHC, IHC-P, WB
FITC Conjugated
100 µl/$459
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
MTHFD1 Rabbit anti-Human Polyclonal (aa863-912) Antibody
Zebrafish, Pig, Horse, Bat, Rabbit, Mouse, Dog, Guinea pig, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100 µl/$485
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
MTHFD1 Rabbit anti-Human Polyclonal (aa863-912) (HRP) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
IHC, IHC-P, WB
HRP Conjugated
100 µl/$459
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
MTHFD1 Rabbit anti-Human Polyclonal (aa863-912) (Biotin) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$459
MTHFD1 Antibody - Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
MTHFD1 Rabbit anti-Human Polyclonal (aa863-912) (FITC) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
IHC, IHC-P, WB
FITC Conjugated
100 µl/$459
Select
MTHFD1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IP, WB
Unconjugated
100 µg/$331
MTHFD1 Antibody - Immunofluorescence staining of MTHFD1 in 293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
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MTHFD1 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ICC, IF, IHC-P
Unconjugated, Preservative Free
50 µl/$275; 100 µl/$312; 200 µl/$350
Select
MTHFD1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IP, WB
Unconjugated
20 µl/$253; 50 µl/$279; 100 µl/$333; 200 µl/$429
MTHFD1 Antibody - Immunofluorescence staining of MTHFD1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
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MTHFD1 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF
Unconjugated
50 µl/$275; 100 µl/$312; 200 µl/$350
MTHFD1 Antibody - Western blot analysis of extracts of HEK293 cells using MTHFD1 antibody. The lane on the left was treated with blocking peptide.
Select
MTHFD1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
100 µl/$369; 200 µl/$411
MTHFD1 Antibody - Detection of human and mouse MTHFD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTHFD1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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MTHFD1 Rabbit anti-Human Polyclonal (aa50-100) Antibody
Mouse, Human
IP, WB
Unconjugated
100 µl/$483
MTHFD1 Antibody - Detection of human and mouse MTHFD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTHFD1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Select
MTHFD1 Rabbit anti-Human Polyclonal (aa150-200) Antibody
Mouse, Human
IP, WB
Unconjugated
100 µl/$483
Select
MTHFD1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
60 µl/$280; 120 µl/$343; 200 µl/$451
MTHFD1 Protein - Purified recombinant protein MTHFD1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
101.4 kDa
20 µg/$1,005
MTHFD1 Protein - 12.5% SDS-PAGE of human MTHFD1 stained with Coomassie Blue
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Wheat Germ Extract
GST
128.59 kDa
10 µg/$469; 25 µg/$630
Select
293T Cells
Myc-DDK (Flag)
101.4 kDa
20 µg/$130
MTHFD1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
101.4 kDa
100 µg/$458
MTHFD1 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
49.0kDa
1 mg/$1,646; 100 µg/$565; 20 µg/$395
Viewing 1-23 of 23 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).