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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MID1

midline 1 (Opitz/BBB syndrome)

The MID1 gene (also called OGS1 and FXY) is ubiquitously expressed in both fetal and adult tissues and displays a transcript of approximately 7 kb that encodes a 667-amino acid protein. The 10-exon human MID1 gene is a member of the RING finger family, characterized by the presence of an N-terminal zinc binding domain. MID1 also contains 4 additional domains: 2 potential zinc binding B box domains, a leucine coiled-coil domain characteristic of the &#039RING-B box-coiled coil&#039 (RBCC) subgroup of RING finger proteins, and a C-terminal domain conserved in several other RBCC proteins. The RING finger family members, including EFP and PML proteins, appear to act as transcription regulators via homeo- and/or heterodimerization. There is a major MID1 RNA species of 7.4 kb and 2 minor RNAs of 4.3 and 2.6 kb. MID1 is located within Xp22.3, proximal to the human pseudoautosomal boundary. It is flanked by 2 previously characterized genes, AMELX and CLCN4. MID1 is the first example of a gene subject to X inactivation in man while escaping it in mouse. MID1 encodes a microtubule-associated protein, called midin, that influences microtubule dynamics in MID1-overexpressing cells. Midin is involved in the formation of multiprotein structures acting as anchor points to microtubules. Impaired association with these cytoskeletal structures causes the developmental defects of Opitz syndrome. Overexpressed MID1 proteins harboring mutations described in Opitz syndrome patients lack the capability to associate with microtubules, forming cytoplasmic clumps instead. Subcellular compartmentalization and the ability to form multiprotein structures both appear to be crucial for the function of this family of proteins. The Opitz G/BBB syndrome, also known as the hypospadias-dysphagia syndrome or telecanthus with associated abnormalities, is associated with midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. There are both X-linked and autosomal dominant forms of this disorder. Although OGS1 maps to Xp22 in approximately the same area as craniofrontonasal dysplasia, they represent separate loci.

Gene Name: midline 1 (Opitz/BBB syndrome)
Synonyms: MID1, GBBB1, Midline 1 RING finger protein, MIDIN, RING finger protein 59, RNF59, Midline 1 (Opitz/BBB syndrome), TRIM18, OGS1, ZNFXY, BBBG1, XPRF, FXY, Midline-1, OS, OSX
Target Sequences: NM_000381 NP_000372.1 O15344

Filtered By: None
Target
MID1 (36)
Reactivity
Human (26)
Mouse (24)
Rat (16)
Bat (8)
Bovine (9)
Chicken (8)
Chimpanzee (6)
Dog (9)
Gibbon (6)
Guinea pig (9)
Horse (9)
Monkey (9)
Orangutan (6)
Pig (9)
Xenopus (8)
Zebrafish (8)
Application
IHC (16)
IHC-P (4)
WB (35)
ELISA (10)
ICC (4)
IF (6)
Peptide-ELISA (1)
Host
rabbit (35)
mouse (1)
Isotype
IgG (26)
IgG2b,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (35)
Clone
2C11 (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (15)
Epitope
aa1-212 (10)
aa575-603 (8)
aa465-514 (4)
aa57-106 (4)
40-120 aa, N-terminal (1)
Glu103 (1)
N-Terminus (1)
aa617-667 (1)
aa71-120 (1)
Publications
No (36)
Showing 1-25 of 36 results

MID1 Antibody (aa71-120) LS-C118672
Rabbit Polyclonal (IgG) to Human MID1
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRI18 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using TRI18 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRI18 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using TRI18 Antibody. The picture on the right is blocked with the synthesized peptide.

MID1 Antibody LS-C416014
Rabbit Polyclonal to Human MID1
Mouse, Rat, Human
ICC IF IHC WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of A549 and HUVEC cell lysate using TRI18 Antibody
Western blot of A549 and HUVEC cell lysate using TRI18 Antibody

MID1 Antibody (N-Terminus) LS-C413053
Rabbit Polyclonal to Human MID1
Mouse, Dog, Rat, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of MID1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MID1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of MID1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MID1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

MID1 Antibody (40-120 aa, N-terminal) LS-C384537
Rabbit Polyclonal (IgG) to Human MID1
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Midline-1 antibody
Western blot of Midline-1 antibody
Western blot of Midline-1 antibody
Western blot of Midline-1 antibody

MID1 Antibody LS-C412832
Rabbit Polyclonal to Human MID1
Rat, Human
IF IHC WB
Unconjugated
100µl  $385.00

MID1 Antibody LS-C346337
Rabbit Polyclonal (IgG) to Human MID1
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of MCF7 cells.
Western blot analysis of extracts of various cells.
Immunofluorescence analysis of MCF7 cells.
Western blot analysis of extracts of various cells.

MID1 Antibody (aa1-212) LS-C372989
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00; 10ml 0.5mg/ml  $2,160.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, HRP) LS-C711739
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
HRP Conjugated
20µl  $215.00; 100µl  $295.00; 200µl  $345.00; 1ml  $695.00; 10ml  $2,805.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, Biotin) LS-C422484
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
ICC WB
Biotin Conjugated
100µl 0.5mg/ml  $375.00

MID1 Antibody (aa1-212, Biotin) LS-C687310
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
Biotin Conjugated
20µl  $205.00; 100µl  $275.00; 200µl  $315.00; 1ml  $590.00; 10ml  $2,370.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, FITC) LS-C422486
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
ICC WB
FITC Conjugated
100µl 0.5mg/ml  $395.00

MID1 Antibody (aa1-212, FITC) LS-C694122
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
FITC Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $335.00; 1ml  $650.00; 10ml  $2,580.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, Cy3) LS-C702715
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
Cy3 Conjugated
20µl  $245.00; 100µl  $365.00; 200µl  $445.00; 1ml  $1,080.00; 10ml  $4,320.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, APC) LS-C740673
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
APC Conjugated
20µl  $225.00; 100µl  $315.00; 200µl  $375.00; 1ml  $815.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, PE) LS-C725799
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
PE Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $335.00; 1ml  $650.00; 10ml  $2,580.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (aa1-212, APC, Cy7) LS-C734749
Rabbit Polyclonal (IgG) to Mouse MID1
Mouse
IHC WB
APC, Cy7 Conjugated
20µl  $285.00; 100µl  $465.00; 200µl  $650.00; 1ml  $1,620.00; 10ml  $6,480.00
Western blot of MID1 antibody.
Western blot of MID1 antibody.

MID1 Antibody (Glu103) LS-C192030
Rabbit Polyclonal to Human MID1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
Immunohistochemistry (IHC) analysis of Midline-1 (E103) pAb in paraffin-embedded human brain tissue.
Immunohistochemistry (IHC) analysis of Midline-1 (E103) pAb in paraffin-embedded human brain tissue.

MID1 Antibody LS-C676050
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA IHC IHC-P
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human brain tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human brain tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100

MID1 Antibody (clone 2C11) LS-C197683
Mouse Monoclonal [clone 2C11] (IgG2b,k) to Human MID1
Human
ELISA WB
Unconjugated
100µg  $355.00

MID1 Antibody (aa575-603) LS-C323000
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA WB
Unconjugated
200µl  $530.00

MID1 Antibody (aa575-603, Biotin) LS-C263152
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

MID1 Antibody (aa575-603, Azide-free, HRP) LS-C263154
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

MID1 Antibody (aa575-603, PE) LS-C263155
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA WB
PE Conjugated
200µl  $680.00

MID1 Antibody (aa575-603, AP) LS-C263150
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA WB
AP Conjugated
200µl  $680.00

MID1 Antibody (aa575-603, APC) LS-C263151
Rabbit Polyclonal (IgG) to Human MID1
Human
ELISA WB
APC Conjugated
200µl  $680.00
Showing 1-25 of 36 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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