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MGMT

O-6-methylguanine-DNA methyltransferase

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene Name: O-6-methylguanine-DNA methyltransferase
Synonyms: MGMT
Target Sequences: NM_002412 NP_002403.2 P16455

Publications (7)

1
Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine. Liu L, Spiro TP, Qin X, Majka S, Haaga J, Schupp J, Willson JK, Gerson SL. Clinical cancer research : an official journal of the American Association for Cancer Research. 2001 7:2318-24. (IHC; Human) [PubMed:11489807]
2
Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection. Roth TJ, Xu Y, Luo M, Kelley MR. Cancer gene therapy. 2003 10:603-10. (WB) [PubMed:12872142]
3
O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Zuo C, Ai L, Ratliff P, Suen JY, Hanna E, Brent TP, Fan CY. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2004 13:967-75. (IHC; Human) [PubMed:15184253]
4
Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents. Cai S, Xu Y, Cooper RJ, Ferkowicz MJ, Hartwell JR, Pollok KE, Kelley MR. Cancer research. 2005 65:3319-27. (WB) [PubMed:15833865]
5
MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?. Della Puppa A, Persano L, Masi G, Rampazzo E, Sinigaglia A, Pistollato F, Denaro L, Barzon L, Pal G, Basso G, Scienza R, d'Avella D. Journal of neuro-oncology. 2012 106:33-41. [PubMed:21725802]
6
Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Molecular carcinogenesis. 2013 52:275-85. (WB; Mouse) [PubMed:22213062]
7
[Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]. Lylo VV, Matsevich LL, Kotsarenko EV, Babenko LA, Korneliuk AI, Sukhorada EM, Lukash LL. TSitologiia i genetika. 45:53-60. [PubMed:22329163]
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MGMT ELISA Kit
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Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Tissue Homogenates
1 Plate/$834
MGMT ELISA Kit
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Sandwich
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Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
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1 Plate/$768
MGMT ELISA Kit
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Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
MGMT ELISA Kit
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Sandwich
96-Well Microplate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$651
MGMT ELISA Kit
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Custom
96-Well Strip Plate
Dog
Colorimetric - 450nm (TMB)
1 Plate/$929
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Custom
96-Well Strip Plate
Rat
Colorimetric - 450nm (TMB)
1 Plate/$783
MGMT ELISA Kit
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Custom
96-Well Strip Plate
Mouse
Colorimetric - 450nm (TMB)
1 Plate/$783
MGMT ELISA Kit
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Custom
96-Well Strip Plate
Colorimetric - 450nm (TMB)
1 Plate/$783
MGMT ELISA Kit
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Competitive EIA
96-Well Strip Plate
Human
0.5 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$913
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96-Well Strip Plate
Rat
78.13 - 5000 pg/ml
Colorimetric - 450nm (TMB)
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1 Plate/$893
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).