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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MELK

maternal embryonic leucine zipper kinase

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

Gene Name: maternal embryonic leucine zipper kinase
Family/Subfamily: Protein Kinase , CAMKL
Synonyms: MELK, KIAA0175, HPK38, Protein kinase Eg3, Tyrosine-protein kinase MELK, Protein kinase PK38, HMELK, Mpk38, PEg3 kinase
Target Sequences: NM_014791 NP_055606.1 Q14680

Filtered By: None
Target
MELK (54)
Reactivity
Human (50)
Mouse (8)
Rat (7)
Bat (1)
Bovine (9)
Chimpanzee (3)
Dog (1)
Gibbon (6)
Guinea pig (8)
Hamster (1)
Horse (6)
Monkey (6)
Orangutan (3)
Pig (5)
Rabbit (5)
Xenopus (1)
Application
IHC (6)
IHC-P (4)
WB (45)
Flo (1)
ELISA (24)
ICC (1)
IF (4)
IP (2)
Peptide-ELISA (4)
Host
rabbit (39)
mouse (8)
goat (7)
Isotype
IgG (26)
IgG1,k (7)
IgG2a (1)
Clonality
monoclonal mc (8)
polyclonal pc (46)
Clone
2G2 (1)
Format
AP Conjugated (4)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (7)
FITC Conjugated (7)
HRP Conjugated (7)
PE Conjugated (4)
Unconjugated (22)
Epitope
aa201-230 (8)
aa525-554 (8)
Internal (5)
aa21-70 (4)
aa432-481 (4)
400-480 aa, Internal (1)
Asn449 (1)
C-Terminus (1)
aa381-415 (1)
aa425-475 (1)
aa431-480 (1)
aa434-468 (1)
aa525-575 (1)
aa550-650 (1)
aa626-630 (1)
aa639-651 (1)
Publications
No (54)
Showing 1-25 of 54 results

MELK Antibody (aa525-575) IHC-plus™ LS-B15485
Rabbit Polyclonal (IgG) to Human MELK
Human
IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.

MELK Antibody (aa425-475) LS-C289836
Rabbit Polyclonal (IgG) to Human MELK
Horse, Human
IP WB
Unconjugated
100µl 1mg/ml  $395.00

MELK Antibody (Internal) LS-C354135
Rabbit Polyclonal to Human MELK
Rat, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MELK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MELK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

MELK Antibody (aa434-468) LS-C356499
Rabbit Polyclonal to Mouse MELK
Mouse
Peptide-ELISA WB
Unconjugated
400µl  $305.00
Anti-Melk Antibody at 1:1000 dilution + mouse ovary lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Melk Antibody at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Melk Antibody at 1:1000 dilution + mouse ovary lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Melk Antibody at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

MELK Antibody (clone 2G2) LS-C169088
Mouse Monoclonal [clone 2G2] (IgG2a) to Human MELK
Human
ELISA Flo IF IHC
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded liver cancer tissues using MELK mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using MELK mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded liver cancer tissues using MELK mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using MELK mouse monoclonal antibody with DAB staining.

MELK Antibody (aa525-554) LS-C98361
Rabbit Polyclonal to Human MELK
Human
WB
Unconjugated
400µl  $305.00
The anti-MELK antibody is used in Western blot to detect MKEL in HEPG2 tissue lysate
The anti-MELK antibody is used in Western blot to detect MKEL in HEPG2 tissue lysate

MELK Antibody (400-480 aa, Internal) LS-C384511
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

MELK Antibody IHC-plus™ LS-B14117
Rabbit Polyclonal (IgG) to Human MELK
ELISA IHC IHC-P WB
Unconjugated
40µg 80µl 0.5mg/ml  $425.00
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

MELK Antibody (Asn449) LS-C176932
Rabbit Polyclonal to Human MELK
Human
IF IHC IHC-P
Unconjugated
100µl  $345.00
IHC of MELK (N449) pAb in paraffin-embedded human liver carcinoma tissue.
IHC of MELK (N449) pAb in paraffin-embedded human liver carcinoma tissue.

MELK Antibody (aa201-230) LS-C327886
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
Unconjugated
200µl  $530.00

MELK Antibody (aa201-230, Biotin) LS-C279439
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

MELK Antibody (aa201-230, FITC) LS-C279446
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
FITC Conjugated
200µl  $680.00

MELK Antibody (aa201-230, AP) LS-C279437
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
AP Conjugated
200µl  $680.00

MELK Antibody (aa201-230, APC) LS-C279438
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
APC Conjugated
200µl  $680.00

MELK Antibody (aa201-230, Azide-free, HRP) LS-C279447
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

MELK Antibody (aa201-230, PE) LS-C279448
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
PE Conjugated
200µl  $680.00

MELK Antibody (aa525-554) LS-C327888
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
Unconjugated
200µl  $530.00

MELK Antibody (aa525-554, Biotin) LS-C279442
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

MELK Antibody (aa525-554, FITC) LS-C279443
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
FITC Conjugated
200µl  $680.00

MELK Antibody (aa525-554, AP) LS-C279440
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
AP Conjugated
200µl  $680.00

MELK Antibody (aa525-554, APC) LS-C279441
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
APC Conjugated
200µl  $680.00

MELK Antibody (aa525-554, Azide-free, HRP) LS-C279444
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

MELK Antibody (aa525-554, PE) LS-C279445
Rabbit Polyclonal (IgG) to Human MELK
Human
ELISA WB
PE Conjugated
200µl  $680.00

MELK Antibody (aa381-415) LS-C356569
Rabbit Polyclonal to Mouse MELK
Mouse
Peptide-ELISA WB
Unconjugated
400µl  $305.00
All lanes : Anti-Melk Antibody at 1:1000 dilution Lane 1: mouse spleen lysates Lane 2: mouse thymus lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-Melk Antibody at 1:1000 dilution Lane 1: mouse spleen lysates Lane 2: mouse thymus lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

MELK Antibody (aa431-480) LS-C119044
Rabbit Polyclonal (IgG) to Human MELK
Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from K562 cells, using MELK Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, using MELK Antibody. The lane on the right is blocked with the synthesized peptide.
Showing 1-25 of 54 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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