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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MDH / MDH2

malate dehydrogenase 2, NAD (mitochondrial)

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.

Gene Name: malate dehydrogenase 2, NAD (mitochondrial)
Synonyms: MDH2, MDH, M-MDH, MGC:3559, MOR1
Target Sequences: NM_005918 NP_005909.2 P40926

Filtered by: None
Target
MDH / MDH2 (37)
Reactivity
Human (37)
Mouse (15)
Rat (8)
Bat (4)
Chimpanzee (3)
Gibbon (3)
Guinea pig (4)
Monkey (4)
Orangutan (3)
Pig (2)
Application
IHC (7)
IHC-P (6)
WB (34)
Flo (1)
ELISA (15)
ICC (2)
IF (5)
IP (1)
Peptide-ELISA (2)
Host
rabbit (31)
mouse (4)
goat (2)
Isotype
IgG (19)
IgG1,k (1)
IgG2a,k (1)
IgG2b,k (2)
Clonality
monoclonal mc (4)
polyclonal pc (33)
Clone
1G12 (1)
2B7 (1)
2D9 (1)
2F5AF8 (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (3)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (21)
Epitope
aa25-338 (8)
aa297-326 (8)
C-Terminus (4)
aa59-338 (2)
aa221-232 (1)
aa40-319 (1)
aa86-115 (1)
internal region (1)
Publications
No (37)
Showing 1-25 of 37 results

IHC-plus™ MDH / MDH2 Antibody (clone 1G12) LS-B6378
Mouse Monoclonal [clone 1G12] (IgG2a,k) to Human MDH / MDH2
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $495.00
Anti-MDH2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
MDH2 monoclonal antibody, clone 1G12. Western blot of MDH2 expression in IMR-32.

MDH / MDH2 Antibody (C-Terminus) LS-C81206
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Guinea pig, Rat, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ MDH / MDH2 Antibody LS-B12912
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of U2OS cells.

IHC-plus™ MDH / MDH2 Antibody (aa40-319) LS-B10823
Rabbit Polyclonal to Human MDH / MDH2
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl 0.38mg/ml  $485.00
Anti-MDH / MDH2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. MDH / MDH2 antibody diluted at 1:10000.

MDH / MDH2 Antibody LS-C482555
Rabbit Polyclonal to Human MDH / MDH2
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunohistochemical analysis of MDH2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MDH2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

MDH / MDH2 Antibody (aa86-115) LS-C164106
Rabbit Polyclonal to Human MDH / MDH2
Mouse, Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MDH2 Antibody western blot of A431,HeLa and mouse NIH/3T3 cell line lysates (35 ug/lane). The MDH2 antibody detected the MDH2 protein (arrow).

MDH / MDH2 Antibody (clone 2F5AF8) LS-C355071
Mouse Monoclonal [clone 2F5AF8] (IgG1,k) to Human MDH / MDH2
Pig, Human
ELISA, ICC, IF, IP
Unconjugated
100µg  $425.00
Immunofluorescence analysis of Mitochondrial Malate Dehydrogenase/MDH2 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MDH2 (2F5AF8) Mouse Monoclonal Antibody at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

MDH / MDH2 Antibody (aa59-338) LS-C376101
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.
Western blot. All lanes: Malate dehydrogenase, mitochondrial antibody at 3 ug/ml. Lane 1: mouse lung tissue. Lane 2: 293T whole cell lysate. Lane 3: K562 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa.

MDH / MDH2 Antibody LS-C768096
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Human
IF, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

MDH / MDH2 Antibody LS-C768097
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Human
IHC, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

MDH / MDH2 Antibody LS-C748564
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Human
IF, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunofluorescence analysis of U2OS cells using MDH2 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MDH2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

MDH / MDH2 Antibody (internal region) LS-C742488
Goat Polyclonal to Human MDH / MDH2
Mouse, Rat, Pig, Human
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Antibody (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1µg/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MDH / MDH2 Antibody (aa221-232) LS-C742535
Goat Polyclonal to Human MDH / MDH2
Human
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Antibody (0.03µg/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.03µg/ml) staining of Human Heart (A) and Liver (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MDH / MDH2 Antibody (aa297-326) LS-C322922
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
Unconjugated
200µl  $590.00

MDH / MDH2 Antibody (aa297-326, Biotin) LS-C262872
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

MDH / MDH2 Antibody (aa297-326, FITC) LS-C262871
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

MDH / MDH2 Antibody (aa297-326, PE) LS-C262875
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
PE Conjugated
200µl  $740.00

MDH / MDH2 Antibody (aa297-326, AP) LS-C262869
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
AP Conjugated
200µl  $740.00

MDH / MDH2 Antibody (aa297-326, APC) LS-C262870
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
APC Conjugated
200µl  $740.00

MDH / MDH2 Antibody (aa297-326, Azide-free, HRP) LS-C262873
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

MDH / MDH2 Antibody LS-C666694
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Human
ELISA, WB
Unconjugated
100µg  $335.00

MDH / MDH2 Antibody LS-C808996
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Western blot Image

MDH / MDH2 Antibody (aa59-338) LS-C376108
Rabbit Polyclonal (IgG) to Human MDH / MDH2
Mouse, Human
ELISA, WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Western blot. All lanes: Malate dehydrogenase, mitochondrial antibody at 4 ug/ml. Lane 1: mouse lung tissue. Lane 2: 293T whole cell lysate. Lane 3: K562 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa.
Western blot All lanes: MDH2 antibody at 4µg/ml Lane 1: Mouse lung tissue Lane 2: 293T whole cell lysate Lane 3: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 31 kDa Observed band size: 36 kDa

MDH / MDH2 Antibody (clone 2B7) LS-C139080
Mouse Monoclonal [clone 2B7] (IgG2b,k) to Human MDH / MDH2
Human
ELISA
Unconjugated
100µg  $365.00
Detection limit for recombinant GST tagged MDH2 is approximately 0.3 ng/ml as a capture antibody.

MDH / MDH2 Antibody (clone 2D9) LS-C139098
Mouse Monoclonal [clone 2D9] (IgG2b,k) to Human MDH / MDH2
Human
ELISA
Unconjugated
100µg  $365.00
Detection limit for recombinant GST tagged MDH2 is approximately 0.1 ng/ml as a capture antibody.
Showing 1-25 of 37 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).