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MCM7

minichromosome maintenance complex component 7

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Gene Name: minichromosome maintenance complex component 7
Synonyms: MCM7, CDC47 homolog, CDC47, Homolog of S. cerevisiae Cdc47, p1.1-MCM3, p1CDC47, p85MCM, PNAS146
Target Sequences: NM_005916 NP_005907.3 P33993

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Proteins (5)
Over-Expression Lysate (2)
Recombinant (3)
MCM7 (5)
No (5)
GST (1)
His (1)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
MCM7 Protein - Purified recombinant protein MCM7 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
81.1 kDa
20 µg/$1,107
MCM7 Protein - 12.5% SDS-PAGE of human MCM7 stained with Coomassie Blue
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Wheat Germ Extract
GST
68.53 kDa
10 µg/$479; 25 µg/$670
MCM7 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
81.1 kDa
100 µg/$494
MCM7 Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
MCM7 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
81.1 kDa
20 µg/$150
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).