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MCM3

minichromosome maintenance complex component 3

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Gene Name: minichromosome maintenance complex component 3
Synonyms: MCM3, DNA replication factor MCM3, HCC5, HRlf beta subunit, p1-MCM3, RLF subunit beta, RLFB, p102, p1.h
Target Sequences: NM_002388 NP_002379.3 P25205

Publications (1)

1
Polo-like kinase 1 depletion induces DNA damage in early S prior to caspase activation. Yim H, Erikson RL. Molecular and cellular biology. 2009 29:2609-21. [PubMed:19289504] [PMC:PMC2682042]

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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
MCM3 (6)
No (6)
GST, N-terminus (1)
His-T7, N-terminus (2)
Myc-DDK (Flag) (3)
Human (4)
Mouse (1)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Wheat Germ Extract (1)
MCM3 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
MCM3 Protein - Purified recombinant protein MCM3 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
90.8 kDa
20 µg/$1,107
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293T Cells
Myc-DDK (Flag)
90.8 kDa
20 µg/$150
MCM3 Protein - Recombinant Minichromosome Maintenance Deficient 3 (MCM3) by SDS-PAGE
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E. coli
His-T7, N-terminus
26.5kDa
10 µg/$267; 50 µg/$352; 200 µg/$702; 100 µg/$562; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
MCM3 Protein - Recombinant Minichromosome Maintenance Deficient 3 (MCM3) by SDS-PAGE
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E. coli
His-T7, N-terminus
26.5kDa
10 µg/$271; 50 µg/$382; 200 µg/$762; 100 µg/$610; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
MCM3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
90.8 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).