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MCF2 / DBL

MCF.2 cell line derived transforming sequence

Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.

Gene Name: MCF.2 cell line derived transforming sequence
Synonyms: MCF2, DBL, Oncogene MCF2 (oncogene DBL), Proto-oncogene MCF-2, ARHGEF21, Proto-oncogene DBL
Target Sequences: NM_005369 NP_005360.3 P10911

Publications (1)

1
Highly variable cancer subpopulations that exhibit enhanced transcriptome variability and metastatic fitness. Nguyen A, Yoshida M, Goodarzi H, Tavazoie SF. Nature communications. 2016 7:11246. (WB; Human) [Full Text Article] [PubMed:27138336] [PMC:PMC4857405] Related Antibodies: LS-C164083.

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Products
Antibodies (17)
Primary (17)
MCF2 / DBL (17)
Human (17)
Mouse (3)
IHC (9)
IHC-P (1)
WB (17)
Flo (7)
ELISA (7)
Peptide-ELISA (1)
rabbit (11)
mouse (6)
IgG (9)
IgG1 (6)
monoclonal mc (6)
polyclonal pc (11)
OTI2C12 (2)
OTI3E4 (2)
OTI7F9 (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (1)
Carrier-free (3)
FITC Conjugated (1)
HRP Conjugated (1)
PE Conjugated (1)
Unconjugated (12)
aa804-833 (7)
aa872-1001 (6)
No (16)
Yes (1)
MCF2 / DBL Antibody - MCF2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) Antibody
Human
ELISA, Flo, IHC, WB
Unconjugated
200 µl/$734
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) (Biotin) Antibody
Human
ELISA, Flo, IHC, WB
Biotin Conjugated
200 µl/$952
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) (FITC) Antibody
Human
ELISA, Flo, IHC, WB
FITC Conjugated
200 µl/$952
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) (APC) Antibody
Human
ELISA, Flo, IHC, WB
APC Conjugated
200 µl/$952
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) (PE) Antibody
Human
ELISA, Flo, IHC, WB
PE Conjugated
200 µl/$952
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MCF2 / DBL Rabbit anti-Human Polyclonal (aa804-833) (Azide-free) (HRP) Antibody
Human
ELISA, Flo, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
MCF2 / DBL Antibody - Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-MCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:500)
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MCF2 / DBL Mouse anti-Human Monoclonal (aa872-1001) (OTI2C12) Antibody
Human
IHC, WB
Unconjugated
100 µl/$434
MCF2 / DBL Antibody - Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-MCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:500)
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MCF2 / DBL Mouse anti-Human Monoclonal (aa872-1001) (Carrier-free) (OTI2C12) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
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MCF2 / DBL Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
MCF2 / DBL Antibody - Western blot analysis of MCF2 expression in K562 cells line lysate. The lane on the left is treated with the antigen-specific peptide.
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MCF2 / DBL Rabbit anti-Human Polyclonal Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
MCF2 / DBL Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY MCF2. (Right lane) cDNA for 48 hrs and lysed
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MCF2 / DBL Mouse anti-Human Monoclonal (aa872-1001) (OTI3E4) Antibody
Human
WB
Unconjugated
100 µl/$434
MCF2 / DBL Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY MCF2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCF2. (1:500)
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MCF2 / DBL Mouse anti-Human Monoclonal (aa872-1001) (Carrier-free) (OTI3E4) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
MCF2 / DBL Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY MCF2. (Right lane) cDNA for 48 hrs and lysed
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MCF2 / DBL Mouse anti-Human Monoclonal (aa872-1001) (OTI7F9) Antibody
Human
WB
Unconjugated
100 µl/$434
MCF2 / DBL Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY MCF2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCF2. (1:500)
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MCF2 / DBL Mouse anti-Human Monoclonal (aa872-1001) (Carrier-free) (OTI7F9) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
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MCF2 / DBL Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
MCF2 / DBL Antibody - Western blot analysis of extracts of HT-29 cells, using MCF2 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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MCF2 / DBL Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
Viewing 1-17 of 17 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).