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MAGEA3

melanoma antigen family A, 3

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Gene Name: melanoma antigen family A, 3
Synonyms: MAGEA3, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, HYPD, MAGE-3 antigen, Melanoma-associated antigen 3, HIP8, MAGE3, Melanoma antigen family A, 3
Target Sequences: U03735 AAA17446.1 P43357

Publications (4)

1
MAGE-C1/CT7 is the dominant cancer-testis antigen targeted by humoral immune responses in patients with multiple myeloma. Curioni-Fontecedro A, Knights AJ, Tinguely M, Nuber N, Schneider C, Thomson CW, von Boehmer L, Bossart W, Pahlich S, Gehring H, Moch H, Renner C, Knuth A, Zippelius A. Leukemia. 2008 22:1646-8. [PubMed:18323799] Related Antibodies: LS-C99934.
2
CD8+ TIL recruitment may revert the association of MAGE A3 with aggressive features in thyroid tumors. Martins MB, Marcello MA, Batista Fde A, Cunha LL, Morari EC, Soares FA, Vassallo J, Ward LS. Journal of immunology research. 2014 2014:921864. (IHC; Human) [Full Text Article] [PubMed:25825704] [PMC:PMC4235601]
3
Landscape mapping of shared antigenic epitopes and their cognate TCRs of tumor-infiltrating T lymphocytes in melanoma. Kenji Murata , Munehide Nakatsugawa , Muhammed A Rahman, Linh T Nguyen, Douglas G Millar, David T Mulder, Kenji Sugata, Hiroshi Saijo, Yukiko Matsunaga, Yuki Kagoya, Tingxi Guo, Mark Anczurowski, Chung-Hsi Wang, Brian D Burt, Dalam Ly, Kayoko Saso, Alexandra Easson, David P Goldstein, Michael Reedijk, Danny Ghazarian, Trevor J Pugh, Marcus O Butler, Tak W Mak, Pamela S Ohashi, Naoto Hirano. eLife. 2020 Apr;9:e53244. [Full Text Article] [PubMed:32314731] [PMC:PMC7234812] Related Antibodies: LS-C99934.
4
Expression and immune responses to MAGE antigens predict survival in epithelial ovarian cancer. Daudi S, Eng KH, Mhawech-Fauceglia P, Morrison C, Miliotto A, Beck A, Matsuzaki J, Tsuji T, Groman A, Gnjatic S, Spagnoli G, Lele S, Odunsi K. PloS one. 9:e104099. [Full Text Article] [PubMed:25101620] [PMC:PMC4125181] Related Antibodies: LS-B4662.
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Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
MAGEA3 (7)
No (7)
6His, N-terminus (1)
GST (1)
GST, N-terminus (1)
His, N-Terminal (1)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (2)
MAGEA3 Protein
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E. coli
6His, N-terminus
50 µg/$411; 250 µg/$985; 10 µg/$276
MAGEA3 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
MAGEA3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
34.6 kDa
20 µg/$150
MAGEA3 Protein - 12.5% SDS-PAGE of human MAGEA3 stained with Coomassie Blue
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Wheat Germ Extract
GST
61.1 kDa
10 µg/$479; 25 µg/$670
MAGEA3 Protein
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E. coli
His, N-Terminal
34.6 kDa
50 µg/$419
MAGEA3 Protein - Purified recombinant protein MAGEA3 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
34.6 kDa
20 µg/$1,107
MAGEA3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
34.6 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).