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LYN

v-yes-1 Yamaguchi sarcoma viral related oncogene homolog

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down-regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down-regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates phosphorylation of the BCR-ABL fusion protein. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3-kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr-72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation.

Gene Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Family/Subfamily: Protein Kinase , Src
Synonyms: LYN, Lyn protein tyrosine kinase, Lyn ptk, Lyn tyrosine kinase, p56Lyn, p53Lyn, Tyrosine-protein kinase Lyn, JTK8, Lyn kinase
Target Sequences: NM_002350 NP_002341.1 P07948

Publications (3)

1
Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Grimmler M, Wang Y, Mund T, Cilensek Z, Keidel EM, Waddell MB, Jkel H, Kullmann M, Kriwacki RW, Hengst L. Cell. 2007 128:269-80. (IP) [PubMed:17254966]
2
Protein purification and preliminary crystallographic analysis of human Lyn tyrosine kinase. Kinoshita T, Miyano N, Nakai R, Yokota K, Ishiguro H, Tada T. Protein expression and purification. 2008 58:318-24. (WB) [PubMed:18272395]
3
Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling. Lee EJ, Jeon MS, Kim BD, Kim JH, Kwon YG, Lee H, Lee YS, Yang JH, Kim TY. Free radical biology & medicine. 2010 48:1133-43. (WB) [PubMed:20123015]

Filtered By: None
Target
LYN (101)
Reactivity
Human (91)
Mouse (65)
Rat (40)
Bat (5)
Bovine (13)
Chicken (2)
Chimpanzee (3)
Dog (8)
Gibbon (6)
Guinea pig (4)
Horse (8)
Monkey (5)
Pig (6)
Rabbit (4)
Sheep (13)
Zebrafish (4)
Application
IHC (47)
IHC-P (12)
WB (94)
Flo (2)
ELISA (29)
ICC (5)
IF (9)
IP (20)
Peptide-ELISA (7)
Host
rabbit (80)
mouse (12)
goat (1)
sheep (8)
Isotype
IgG (64)
IgG1 (7)
IgG1,k (1)
IgG2a (1)
IgG2a,k (1)
IgG2b (1)
Clonality
monoclonal mc (12)
polyclonal pc (89)
Clone
1174CT22.3.1.1 (1)
2H8D7 (1)
LYN-01 (3)
Format
AP Conjugated (5)
APC (1)
APC Conjugated (5)
Azide-free (2)
Biotin Conjugated (10)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (10)
HRP Conjugated (9)
PE Conjugated (6)
Unconjugated (54)
Epitope
aa263-491 (10)
aa1-30 (8)
aa32-62 (8)
aa35-84 (4)
aa43-92 (4)
C-Terminus (3)
aa8-238 (3)
pTyr192 (3)
pTyr507 (3)
440-520 aa (2)
aa39-58 (2)
pTyr508 (2)
N-Terminus (1)
Thr501 (1)
aa1-210 (1)
aa1-50 (1)
aa149-198 (1)
aa20-30 (1)
aa205-239 (1)
aa229-263 (1)
aa43-77 (1)
aa459-508 (1)
aa463-512 (1)
Publications
No (101)
Showing 1-25 of 101 results

LYN Antibody (aa39-58) IHC-plus™ LS-B7849
Mouse Monoclonal (IgG2a,k) to Human LYN
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
50µl  $425.00
Anti-LYN antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-LYN antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-LYN antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-LYN antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

LYN Antibody (C-Terminus) LS-C368649
Rabbit Polyclonal to Human LYN
Chicken, Sheep, Bovine, Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of LYN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of LYN expression in A431 (A); HeLa (B); mouse kidney (C); PC12 (D) whole cell lysates.
Immunohistochemical analysis of LYN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of LYN expression in A431 (A); HeLa (B); mouse kidney (C); PC12 (D) whole cell lysates.

LYN Antibody (aa229-263) LS-C344391
Rabbit Polyclonal to Human LYN
Rat, Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Immunohistochemical of paraffin-embedded M.spleen section using Mouse Lyn Antibody. NA was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot of lysates from Raji cell line and rat spleen tissue lysate (from left to right) with Mouse Lyn Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Immunohistochemical of paraffin-embedded M.spleen section using Mouse Lyn Antibody. NA was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot of lysates from Raji cell line and rat spleen tissue lysate (from left to right) with Mouse Lyn Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

LYN Antibody (C-Terminus) LS-C368407
Rabbit Polyclonal to Human LYN
Mouse, Sheep, Bovine, Rat, Pig, Human
ICC IF IHC IHC-P WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of LYN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of LYN staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of LYN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of LYN staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

LYN Antibody (C-Terminus) LS-C368402
Rabbit Polyclonal to Human LYN
Mouse, Sheep, Bovine, Rat, Pig, Human
ICC IF IHC IHC-P WB
Unconjugated
30µl 1mg/ml  $215.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $325.00
Immunohistochemical analysis of LYN (pY397) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of LYN (pY397) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of LYN (pY397) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of LYN (pY397) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

LYN Antibody LS-C747181
Rabbit Polyclonal (IgG) to Human LYN
Human
Flo IF IHC IP WB
Unconjugated
50µl  $265.00

LYN Antibody (aa32-62) LS-C99383
Rabbit Polyclonal to Human LYN
Mouse, Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-LYN Antibody in HL60 cell line lysates (35 ug/lane). LYN (arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-LYN Antibody in HL60 cell line lysates (35 ug/lane). LYN (arrow) was detected using the purified antibody.

LYN Antibody (N-Terminus) LS-C8560
Rabbit Polyclonal (IgG) to Human LYN
Mouse, Rat, Human
ELISA IHC IHC-P IP WB
Unconjugated
100µl  $530.00

LYN Antibody LS-C387088
Rabbit Polyclonal (IgG) to Human LYN
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemistry of Lyn antibody
Immunohistochemistry of Lyn antibody
Immunohistochemistry of Lyn antibody
Immunohistochemistry of Lyn antibody

LYN Antibody (aa1-30) LS-C322783
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
Unconjugated
200µl  $530.00

LYN Antibody (aa1-30, Biotin) LS-C262387
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

LYN Antibody (aa1-30, FITC) LS-C262388
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
FITC Conjugated
200µl  $680.00

LYN Antibody (aa1-30, AP) LS-C262386
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
AP Conjugated
200µl  $680.00

LYN Antibody (aa1-30, APC) LS-C262385
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
APC Conjugated
200µl  $680.00

LYN Antibody (aa1-30, Azide-free, HRP) LS-C262389
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

LYN Antibody (aa1-30, PE) LS-C262392
Rabbit Polyclonal (IgG) to Mouse LYN
Mouse
ELISA IHC WB
PE Conjugated
200µl  $680.00

LYN Antibody (aa463-512) LS-C117568
Rabbit Polyclonal (IgG) to Human LYN
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Lyn Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO cells, treated with H2O2 100uM 30', using Lyn Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Lyn Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO cells, treated with H2O2 100uM 30', using Lyn Antibody. The lane on the right is blocked with the synthesized peptide.

LYN Antibody (phospho-Tyr507) LS-C199208
Rabbit Polyclonal (IgG) to Human LYN
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using Lyn (Phospho-Tyr507) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using Lyn (Phospho-Tyr507) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Lyn (Phospho-Tyr507) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using Lyn (Phospho-Tyr507) Antibody. The lane on the right is blocked with the phospho peptide.

LYN Antibody LS-C751675
Mouse Monoclonal (IgG2a) to Human LYN
Human
ELISA IHC WB
Unconjugated
100µg  $355.00

LYN Antibody (aa32-62) LS-C327790
Rabbit Polyclonal (IgG) to Human LYN
Mouse, Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

LYN Antibody (aa8-238, clone LYN-01) LS-C44852
Mouse Monoclonal [clone LYN-01] (IgG1) to Human LYN
Mouse, Rat, Human
IF IP WB
Unconjugated
100µg 1mg/ml  $335.00

LYN Antibody LS-C751673
Rabbit Polyclonal (IgG) to Human LYN
Human
ELISA IHC WB
Unconjugated
100µg  $375.00

LYN Antibody (440-520 aa) LS-C384411
Rabbit Polyclonal (IgG) to Human LYN
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Lyn antibody
Western blot of Lyn antibody

LYN Antibody (aa1-30) LS-C164441
Rabbit Polyclonal to Mouse LYN
Mouse
IHC IHC-P WB
Unconjugated
400µl  $305.00
Mouse Lyn Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse bone(femur) followed by peroxidase-conjugated secondary antibody and DAB staining.
Mouse Lyn Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The Lyn antibody detected the Lyn protein (arrow).
Mouse Lyn Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse bone(femur) followed by peroxidase-conjugated secondary antibody and DAB staining.
Mouse Lyn Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The Lyn antibody detected the Lyn protein (arrow).

LYN Antibody (aa8-238, clone LYN-01) LS-C202425
Mouse Monoclonal [clone LYN-01] (IgG) to Human LYN
Human
ICC IP WB
Unconjugated
100µg 1mg/ml  $485.00
Showing 1-25 of 101 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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