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KPNA2 / Importin Alpha 1

karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.

Gene Name: karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Synonyms: KPNA2, Importin alpha 2, Importin subunit alpha-2, IPOA1, Importin alpha-2 subunit, Karyopherin subunit alpha-2, Importin-alpha-P1, Qip2, RAG cohort 1, SRP1, RCH1, SRP1-alpha, SRP1alpha, Importin alpha 1, Pendulin, RAG cohort protein 1
Target Sequences: NM_002266 NP_002257.1 P52292

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Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
KPNA2 / Importin Alpha 1 (9)
No (9)
6His, N-terminus (2)
GST (1)
GST, N-terminus (1)
His (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (8)
293T Cells (1)
E. coli (4)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (3)
Low endotoxin level (2)
KPNA2 / Importin Alpha 1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
KPNA2 / Importin Alpha 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
57.7 kDa
100 µg/$494
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E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
60.0 kDa
10 µg/$344; 50 µg/$584
KPNA2 / Importin Alpha 1 Protein - 12.5% SDS-PAGE of human KPNA2 stained with Coomassie Blue
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Wheat Germ Extract
GST
83.93 kDa
10 µg/$479; 25 µg/$670
KPNA2 / Importin Alpha 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
57.7 kDa
20 µg/$150
KPNA2 / Importin Alpha 1 Protein - Purified recombinant protein KPNA2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
57.7 kDa
20 µg/$1,107
KPNA2 / Importin Alpha 1 Protein
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E. coli
His
50 µg/$361; 250 µg/$739; 10 µg/$261
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E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
60.04kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
KPNA2 / Importin Alpha 1 Protein - Recombinant Karyopherin Alpha 2 By SDS-PAGE
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E. coli
His-T7
31.1 kDa
10 µg/$279; 50 µg/$432; 100 µg/$690; 200 µg/$862; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).