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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KIAA0652 / ATG13

autophagy related 13

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Gene Name: autophagy related 13
Synonyms: ATG13, Autophagy-related protein 13, KIAA0652, Autophagy related 13
Target Sequences: NM_014741 NP_001136145.1 O75143

Publications (1)

1
Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy. Joo JH, Dorsey FC, Joshi A, Hennessy-Walters KM, Rose KL, McCastlain K, Zhang J, Iyengar R, Jung CH, Suen DF, Steeves MA, Yang CY, Prater SM, Kim DH, Thompson CB, Youle RJ, Ney PA, Cleveland JL, Kundu M. Molecular cell. 2011 43:572-85. [PubMed:21855797] [PMC:PMC3485687] Related Antibodies: LS-C153855, LS-C153856.


KIAA0652 / ATG13 Antibody - Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
KIAA0652 / ATG13 Antibody - Human Colon, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal to Human KIAA0652 / ATG13
Human
IHC, IHC-P, WB
Unconjugated
200µl   $425.00
KIAA0652 / ATG13 Antibody - Anti-KIAA0652 / ATG13 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
KIAA0652 / ATG13 Antibody - Western blot of affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, Flo, WB
Unconjugated
100µg   1.1mg/ml   $425.00
KIAA0652 / ATG13 Antibody - Western blot of affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, WB
Unconjugated
100µg   1.13mg/ml   $425.00
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibodyat dilution of 1:100 (40x lens).
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IF, IHC, WB
Unconjugated
100µg   $405.00
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
Select
Mouse Monoclonal [clone 6B2E12] (IgG1) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
Unconjugated
100µl   $365.00
KIAA0652 / ATG13 Antibody - Immunofluorescent analysis of ATG13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Select
Rabbit Polyclonal to Human KIAA0652 / ATG13
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
50µl   1mg/ml   $255.00; 100µl   1mg/ml   $295.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
APC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl   $740.00
KIAA0652 / ATG13 Antibody - Western blot using the affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto and probed with the primary antibody at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, Flo, WB
Unconjugated
25µl   1.1mg/ml   $235.00
KIAA0652 / ATG13 Antibody - 1:100 staining human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
KIAA0652 / ATG13 Antibody - 1:100 staining human thyroid carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.
Select
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Human
ELISA, IHC, IHC-P
Unconjugated
50µl   $285.00; 100µl   $385.00
Select
Mouse Monoclonal [clone 3E4C8] (IgG1) to Human KIAA0652 / ATG13
Human
ELISA, Flo
Unconjugated
100µl   $365.00
KIAA0652 / ATG13 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite.
Select
Rabbit Polyclonal to Human KIAA0652 / ATG13
Mouse, Human
IF, WB
Unconjugated
100µg   1mg/ml   $365.00
KIAA0652 / ATG13 Antibody - Western blot of ATG13 pAb in extracts from Human brain tissue.
Select
Rabbit Polyclonal to Human KIAA0652 / ATG13
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl   $385.00
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).