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KIAA0652 / ATG13

autophagy related 13

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Gene Name: autophagy related 13
Synonyms: ATG13, Autophagy-related protein 13, KIAA0652, Autophagy related 13
Target Sequences: NM_014741 NP_001136145.1 O75143

Publications (1)

1
Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy. Joo JH, Dorsey FC, Joshi A, Hennessy-Walters KM, Rose KL, McCastlain K, Zhang J, Iyengar R, Jung CH, Suen DF, Steeves MA, Yang CY, Prater SM, Kim DH, Thompson CB, Youle RJ, Ney PA, Cleveland JL, Kundu M. Molecular cell. 2011 43:572-85. [PubMed:21855797] [PMC:PMC3485687] Related Antibodies: LS-C153855, LS-C153856.

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Antibodies (68)
Primary (68)
KIAA0652 / ATG13 (68)
Human (68)
Mouse (24)
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IHC (17)
IHC-P (8)
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IP (1)
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rabbit (64)
mouse (4)
Product Group
IHCPlus (3)
IgG (37)
IgG1 (4)
monoclonal mc (4)
polyclonal pc (64)
3E4C8 (1)
6B2E12 (1)
OTI1B1 (2)
AP Conjugated (1)
APC Conjugated (4)
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aa333-362 (7)
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N-Terminus (4)
aa80-129 (4)
aa285-364 (2)
pSer318 (2)
C-Terminus (1)
aa318-517 (1)
aa450-500 (1)
aa488-517 (1)
No (66)
Yes (2)
KIAA0652 / ATG13 Antibody - Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ KIAA0652 / ATG13 Antibody LS-B5277
(4.0) Rating
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
KIAA0652 / ATG13 Antibody - Western blot of affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
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KIAA0652 / ATG13 Antibody (phospho-Ser318) LS-C153855
(4.0) Rating
(1) Publication(s)
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (pSer318) Antibody
Human
ELISA, Flo, WB
Unconjugated
100 µg/$567
KIAA0652 / ATG13 Antibody - Western blot of affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
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KIAA0652 / ATG13 Antibody LS-C153856
(4.0) Rating
(1) Publication(s)
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$567
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibodyat dilution of 1:100 (40x lens).
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KIAA0652 / ATG13 Antibody LS-C331070
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
KIAA0652 / ATG13 Antibody - Western blot using the affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto and probed with the primary antibody at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.
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KIAA0652 / ATG13 Antibody (phospho-Ser318) LS-C745344
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (pSer318) Antibody
Human
ELISA, Flo, WB
Unconjugated
25 µl/$304
KIAA0652 / ATG13 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite.
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KIAA0652 / ATG13 Antibody (Biotin) LS-C773977
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (Biotin) Antibody
Mouse, Human
IF, WB
Biotin Conjugated
100 µg/$477
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KIAA0652 / ATG13 Antibody LS-C659250
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$331
KIAA0652 / ATG13 Antibody - Human Colon, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
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IHC-plus™ KIAA0652 / ATG13 Antibody (aa1-30) LS-B11831
(4.0) Rating
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Human
IHC, IHC-P, WB
Unconjugated
200 µl/$460
KIAA0652 / ATG13 Antibody - Anti-KIAA0652 / ATG13 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ KIAA0652 / ATG13 Antibody (C-Terminus) LS-B10936
(4.0) Rating
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
0.05 mg/$495
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
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KIAA0652 / ATG13 Antibody LS-C677219
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
KIAA0652 / ATG13 Antibody LS-C764688
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
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KIAA0652 / ATG13 Antibody (clone 6B2E12) LS-C669390
KIAA0652 / ATG13 Mouse anti-Human Monoclonal (6B2E12) Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µl/$459
KIAA0652 / ATG13 Antibody - Immunofluorescent analysis of ATG13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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KIAA0652 / ATG13 Antibody LS-C482241
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
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KIAA0652 / ATG13 Antibody (aa1-30) LS-C319663
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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KIAA0652 / ATG13 Antibody (aa1-30, Biotin) LS-C232949
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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KIAA0652 / ATG13 Antibody (aa1-30, FITC) LS-C232950
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
Select
KIAA0652 / ATG13 Antibody (aa1-30, APC) LS-C232948
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
Select
KIAA0652 / ATG13 Antibody (aa1-30, PE) LS-C232951
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
Select
KIAA0652 / ATG13 Antibody (aa1-30, Azide-free, HRP) LS-C249481
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa1-30) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.
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KIAA0652 / ATG13 Antibody (aa318-517) LS-C376017
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (aa318-517) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
Select
KIAA0652 / ATG13 Antibody (clone 3E4C8) LS-C669389
KIAA0652 / ATG13 Mouse anti-Human Monoclonal (3E4C8) Antibody
Human
ELISA, Flo
Unconjugated
100 µl/$459
KIAA0652 / ATG13 Antibody - 1:100 staining human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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KIAA0652 / ATG13 Antibody LS-C805990
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
KIAA0652 / ATG13 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite.
Select
KIAA0652 / ATG13 Antibody LS-C773976
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
100 µg/$442
KIAA0652 / ATG13 Antibody - Western blot of ATG13 pAb in extracts from Human brain tissue.
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KIAA0652 / ATG13 Antibody LS-C192266
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
KIAA0652 / ATG13 Antibody - Detection of Atg13 in formaldehyde-fixed C2C12 cells.
Select
KIAA0652 / ATG13 Antibody LS-C759885
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
100 µg/$537
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