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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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JUNB / JUN-B

jun B proto-oncogene

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Gene Name: jun B proto-oncogene
Synonyms: JUNB, Activator protein 1, AP-1, Jun B proto-oncogene, G0s3, Transcription factor jun-B
Target Sequences: NM_002229 NP_002220.1 P17275

Filtered By: None
Target
JUNB / JUN-B (85)
Reactivity
Human (82)
Mouse (48)
Rat (43)
Bat (17)
Bovine (20)
Chimpanzee (9)
Dog (21)
Guinea pig (13)
Hamster (1)
Horse (22)
Monkey (22)
Pig (9)
Sheep (12)
Xenopus (8)
Zebrafish (8)
Application
IHC (30)
IHC-P (16)
WB (74)
ChrIP (1)
DB (2)
ELISA (13)
ICC (8)
IF (9)
IP (20)
Peptide-ELISA (5)
Host
rabbit (79)
mouse (5)
goat (1)
Isotype
IgG (66)
IgG1 (4)
Clonality
monoclonal mc (5)
polyclonal pc (80)
Clone
204C4a (2)
TS106 (1)
Format
AP Conjugated (4)
APC Conjugated (4)
Azide-free (1)
Biotin Conjugated (8)
FITC Conjugated (8)
HRP Conjugated (8)
PE Conjugated (4)
Unconjugated (49)
Epitope
aa249-276 (8)
pSer259 (5)
pSer79 (5)
N-Terminus (4)
aa20-69 (4)
aa241-290 (4)
aa271-320 (4)
20-100 aa (2)
200-280 aa (2)
C-Terminus (2)
Internal (2)
Asp253 (1)
Gly73 (1)
aa1-240 (1)
aa1-40 (1)
aa145-246 (1)
aa226-275 (1)
aa302-315 (1)
aa323-347 (1)
aa40-80 (1)
aa46-95 (1)
Publications
No (85)
Showing 1-25 of 85 results

JUNB / JUN-B Antibody (aa226-275) IHC-plus™ LS-B7011
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Human
IHC IHC-P IP Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-JUNB antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from HeLa cells, using JunB Antibody. The lane on the right is blocked with the synthesized peptide.
Anti-JUNB antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from HeLa cells, using JunB Antibody. The lane on the right is blocked with the synthesized peptide.

JUNB / JUN-B Antibody (C-Terminus) LS-C356138
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Sheep, Bovine, Rat, Human
ICC IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of JUNB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of JUNB staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of JUNB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of JUNB staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

JUNB / JUN-B Antibody LS-C748047
Rabbit Monoclonal (IgG) to Human JUNB / JUN-B
Human
WB
Unconjugated
50µl  $265.00

JUNB / JUN-B Antibody LS-C666537
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $335.00

JUNB / JUN-B Antibody (aa1-40) LS-C289427
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human JunB by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-JunB used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Detection of Human JunB by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-JunB used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

JUNB / JUN-B Antibody (aa40-80) LS-C289430
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Human
IF IHC IHC-P IP
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human JunB by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-JunB used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Detection of Human JunB by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-JunB used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.

JUNB / JUN-B Antibody (20-100 aa) LS-C384244
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
ELISA IHC IP WB
Unconjugated
100µl 1mg/ml  $265.00

JUNB / JUN-B Antibody (200-280 aa) LS-C380759
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
ELISA IHC IP WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Phospho-Jun B (S259) antibody
Western blot of Phospho-Jun B (S259) antibody

JUNB / JUN-B Antibody (200-280 aa) LS-C384243
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Human
ELISA IHC IP WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Jun B antibody
Western blot of Jun B antibody

JUNB / JUN-B Antibody LS-C355657
Rabbit Polyclonal (IgG) to Mouse JUNB / JUN-B
Mouse, Hamster
ChrIP IF IP WB
Unconjugated
200µg 200µl  $385.00
Immunofluorescence analysis of JunB was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JunB Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Chromatin immunoprecipitation analysis of JunB was performed using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a JunB polyclonal antibody. Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of the LAMC1 gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat LAMC1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.
Immunofluorescence analysis of JunB was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JunB Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Chromatin immunoprecipitation analysis of JunB was performed using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a JunB polyclonal antibody. Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of the LAMC1 gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat LAMC1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.

JUNB / JUN-B Antibody (aa323-347) LS-C344078
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Guinea pig, Rat, Pig, Horse, Bat, Human, Monkey
IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
JunB antibody IHC-paraffin: Mouse Brain Tissue.
JunB antibody IHC-paraffin: Rat Brain Tissue.
JunB antibody IHC-paraffin: Mouse Brain Tissue.
JunB antibody IHC-paraffin: Rat Brain Tissue.

JUNB / JUN-B Antibody (Gly73) LS-C176088
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl  $345.00
IHC of JunB (G73) pAb in paraffin-embedded human breast carcinoma tissue.
IHC of JunB (G73) pAb in paraffin-embedded human breast carcinoma tissue.

JUNB / JUN-B Antibody LS-C346076
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of HeLa cells.
Immunofluorescence analysis of HeLa cells.

JUNB / JUN-B Antibody (phospho-Ser259) LS-C117401
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
IHC IHC-P IP Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.

JUNB / JUN-B Antibody (Asp253) LS-C176087
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Human
IHC IHC-P IP WB
Unconjugated
100µl  $345.00
IHC of JunB (D253) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of JunB (D253) pAb in extracts from HeLa cells.
IHC of JunB (D253) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of JunB (D253) pAb in extracts from HeLa cells.

JUNB / JUN-B Antibody (phospho-Ser259) LS-C177918
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl  $345.00
IHC of p-JunB (S259) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of p-JunB (S259) pAb in extracts from HeLa cells.
IHC of p-JunB (S259) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of p-JunB (S259) pAb in extracts from HeLa cells.

JUNB / JUN-B Antibody (Internal) LS-C368983
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Bovine, Rat, Human
IHC IHC-P IP WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of JUNB staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of JUNB expression in MCF7 (A); HeLa (B); RAW264.7 (C) whole cell lysates.
Immunohistochemical analysis of JUNB staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of JUNB expression in MCF7 (A); HeLa (B); RAW264.7 (C) whole cell lysates.

JUNB / JUN-B Antibody (C-Terminus) LS-C356141
Rabbit Polyclonal to Human JUNB / JUN-B
Mouse, Sheep, Bovine, Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of JUNB (pS259) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of JUNB (pS259) expression in HeLa (A); MCF7 (B) whole cell lysates.
Immunohistochemical analysis of JUNB (pS259) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of JUNB (pS259) expression in HeLa (A); MCF7 (B) whole cell lysates.

JUNB / JUN-B Antibody (phospho-Ser79) LS-C117402
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
IHC IHC-P IP Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser79) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser79) Antibody. The picture on the right is blocked with the phospho peptide.

JUNB / JUN-B Antibody (aa46-95) LS-C118168
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
IHC IHC-P IP Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using JunB Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using JunB Antibody. The picture on the right is blocked with the synthesized peptide.

JUNB / JUN-B Antibody (phospho-Ser79) LS-C123913
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Dog, Bovine, Rat, Horse, Human, Monkey
IF IHC
Unconjugated
50µg  $435.00

JUNB / JUN-B Antibody LS-C123915
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Dog, Bovine, Rat, Horse, Human, Monkey
IF IHC
Unconjugated
50µg  $435.00

JUNB / JUN-B Antibody (20-100 aa) LS-C380756
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Mouse, Rat, Human
ELISA IHC IP
Unconjugated
100µl 1mg/ml  $265.00

JUNB / JUN-B Antibody (phospho-Ser79) LS-C336018
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
IF IHC
Unconjugated
50µl  $265.00; 100µl  $375.00; 200µl  $495.00
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed Hela cells.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed Hela cells.

JUNB / JUN-B Antibody (aa1-240) LS-C375997
Rabbit Polyclonal (IgG) to Human JUNB / JUN-B
Human
ELISA IHC IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Showing 1-25 of 85 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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