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HYPE / FICD

FIC domain containing

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.

Gene Name: FIC domain containing
Synonyms: FICD, AMPylator FICD, FIC domain containing, FIC domain-containing protein, HIP13, Huntingtin yeast partner E, UNQ3041, HIP-13, HYPE
Target Sequences: NM_007076 NP_009007.2 Q9BVA6

Publications (1)

1
AMPylation matches BiP activity to client protein load in the endoplasmic reticulum. Preissler S, Rato C, Chen R, Antrobus R, Ding S, Fearnley IM, Ron D. eLife. 2015 4:e12621. (WB; Human) [Full Text Article] [PubMed:26673894] [PMC:PMC4739761]

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Products
Antibodies (34)
Primary (34)
HYPE / FICD (34)
Human (34)
Mouse (7)
Rat (7)
Bat (6)
Bovine (6)
Chimpanzee (3)
Dog (6)
Drosophila (3)
Gibbon (6)
Guinea pig (3)
Horse (6)
Monkey (6)
Pig (6)
Rabbit (3)
Zebrafish (3)
IHC (5)
IHC-P (5)
WB (29)
ELISA (7)
IF (1)
Peptide-ELISA (1)
rabbit (14)
mouse (19)
goat (1)
Product Group
IHCPlus (1)
IgG (15)
IgG1 (12)
IgG2a,k (6)
monoclonal mc (18)
polyclonal pc (16)
OTI11E11 (2)
OTI15E10 (2)
OTI2A11 (2)
OTI2A12 (2)
OTI3E3 (2)
OTI3G3 (2)
APC Conjugated (1)
Biotin Conjugated (4)
Carrier-free (6)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (20)
aa61-265 (12)
C-Terminus (4)
aa351-400 (4)
Internal Region (1)
No (34)
HYPE / FICD Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HYPE / FICD Antibody (C-Terminus) LS-B12622
HYPE / FICD Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
HYPE / FICD Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HYPE / FICD Antibody (C-Terminus, HRP) LS-C449304
HYPE / FICD Rabbit anti-Human Polyclonal (C-Terminus) (HRP) Antibody
Mouse, Dog, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
HYPE / FICD Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HYPE / FICD Antibody (C-Terminus, Biotin) LS-C449302
HYPE / FICD Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody
Mouse, Dog, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
HYPE / FICD Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HYPE / FICD Antibody (C-Terminus, FITC) LS-C449303
HYPE / FICD Rabbit anti-Human Polyclonal (C-Terminus) (FITC) Antibody
Mouse, Dog, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
HYPE / FICD Antibody - Immunohistochemistry image at a dilution of 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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HYPE / FICD Antibody LS-C673395
HYPE / FICD Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
HYPE / FICD Antibody LS-C307548
HYPE / FICD Mouse anti-Human Monoclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$705
Select
HYPE / FICD Antibody (HRP) LS-C542165
HYPE / FICD Mouse anti-Human Monoclonal (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
100 µl/$904
Select
HYPE / FICD Antibody (Biotin) LS-C522031
HYPE / FICD Mouse anti-Human Monoclonal (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
100 µl/$904
Select
HYPE / FICD Antibody (FITC) LS-C532075
HYPE / FICD Mouse anti-Human Monoclonal (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
100 µl/$904
Select
HYPE / FICD Antibody (PE) LS-C650275
HYPE / FICD Mouse anti-Human Monoclonal (PE) Antibody
Human
ELISA, WB
PE Conjugated
100 µl/$904
Select
HYPE / FICD Antibody (APC) LS-C513816
HYPE / FICD Mouse anti-Human Monoclonal (APC) Antibody
Human
ELISA, WB
APC Conjugated
100 µl/$904
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI2A12) LS-C336706
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (OTI2A12) Antibody
Human
WB
Unconjugated
100 µl/$434
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI2A12, Carrier-free) LS-C789668
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (Carrier-free) (OTI2A12) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI15E10) LS-C336735
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (OTI15E10) Antibody
Human
WB
Unconjugated
100 µl/$434
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI15E10, Carrier-free) LS-C789701
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (Carrier-free) (OTI15E10) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI3E3) LS-C336733
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (OTI3E3) Antibody
Human
WB
Unconjugated
100 µl/$434
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI3E3, Carrier-free) LS-C789705
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (Carrier-free) (OTI3E3) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI11E11) LS-C338609
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (OTI11E11) Antibody
Human
WB
Unconjugated
100 µl/$434
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI11E11, Carrier-free) LS-C789700
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (Carrier-free) (OTI11E11) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI3G3) LS-C338612
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (OTI3G3) Antibody
Human
WB
Unconjugated
100 µl/$434
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI3G3, Carrier-free) LS-C789703
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (Carrier-free) (OTI3G3) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI2A11) LS-C340556
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (OTI2A11) Antibody
Human
WB
Unconjugated
100 µl/$434
HYPE / FICD Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FICD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD.
Select
HYPE / FICD Antibody (aa61-265, clone OTI2A11, Carrier-free) LS-C789669
HYPE / FICD Mouse anti-Human Monoclonal (aa61-265) (Carrier-free) (OTI2A11) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
HYPE / FICD Antibody - Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HYPE / FICD Antibody (aa351-400) LS-C80940
HYPE / FICD Rabbit anti-Human Polyclonal (aa351-400) Antibody
Human
WB
Unconjugated
100 µl/$397
HYPE / FICD Antibody - Western blot analysis of extracts of various cell lines, using FICD antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
Select
HYPE / FICD Antibody LS-C748785
HYPE / FICD Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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