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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HLA-DRB1

Major histocompatibility complex, class II, DR beta 1

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Gene Name: Major histocompatibility complex, class II, DR beta 1
Synonyms: HLA-DRB1, DRB1, HLA-DR1B, HLA-DRB, Human leucocyte antigen DRB1, MHC class II antigen DRB1*10, MHC class II antigen, MHC class II antigen DRB1*1, HLA-DRB2, Lymphocyte antigen DRB1, SS1
Target Sequences: NM_002124 NP_002115.2 P01911

Filtered By: None
Target
HLA-DRB1 (68)
Reactivity
Human (58)
Mouse (11)
Chimpanzee (2)
Dog (2)
Monkey (17)
Application
IHC (40)
IHC-P (23)
WB (66)
Flo (24)
ELISA (12)
ICC (5)
IF (25)
Host
rabbit (44)
mouse (24)
Isotype
IgG (44)
IgG2a,k (2)
IgG2b,k (18)
Clonality
monoclonal mc (23)
polyclonal pc (45)
Clone
HLA-DRB/1067 (3)
L243 (2)
LN3 (3)
LN3 + HLA-DRB/1067 (3)
MHDRb2 (3)
SPM288 (3)
SPM289 (3)
SPM423 (3)
Format
AP Conjugated (2)
APC (2)
APC Conjugated (4)
Azide-free (9)
Biotin Conjugated (7)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (7)
HRP Conjugated (5)
PE Conjugated (4)
Ready-to-Use (7)
Unconjugated (35)
Epitope
aa30-266 (14)
aa27-264 (10)
aa30-58 (8)
aa103-137 (1)
aa122-154 (1)
Publications
No (68)
Showing 1-25 of 68 results

HLA-DRB1 Antibody (clone LN3) IHC-plus™ LS-B15867
Mouse Monoclonal [clone LN3] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
50µg 0.2mg/ml  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:50.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:50.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:50.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:50.

HLA-DRB1 Antibody (clone LN3, Azide-free) LS-C390318
Mouse Monoclonal [clone LN3] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
IHC analysis of human histiocytoma and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC analysis of human tonsil and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC analysis of human histiocytoma and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC analysis of human tonsil and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone LN3, Ready-to-Use) LS-C390323
Mouse Monoclonal [clone LN3] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
IHC analysis of human histiocytoma and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC analysis of human tonsil and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC analysis of human histiocytoma and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC analysis of human tonsil and HLA-DRB1 antibody (clone LN3).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone HLA-DRB/1067) LS-C390340
Mouse Monoclonal [clone HLA-DRB/1067] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg  $395.00
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone HLA-DRB/1067, Ready-to-Use) LS-C390341
Mouse Monoclonal [clone HLA-DRB/1067] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone HLA-DRB/1067, Azide-free) LS-C390342
Mouse Monoclonal [clone HLA-DRB/1067] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone MHDRb2) LS-C391951
Mouse Monoclonal [clone MHDRb2] (IgG2b,k) to Human HLA-DRB1
Human
Flo IF IHC IHC-P WB
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg 0.2mg/ml  $395.00
IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone MHDRb2, Ready-to-Use) LS-C391947
Mouse Monoclonal [clone MHDRb2] (IgG2b,k) to Human HLA-DRB1
Human
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone MHDRb2, Azide-free) LS-C391953
Mouse Monoclonal [clone MHDRb2] (IgG2b,k) to Human HLA-DRB1
Human
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (aa30-58, Biotin) LS-C219723
Rabbit Polyclonal (IgG) to Human HLA-DRB1
Human
ELISA WB
Biotin Conjugated
200µl 0.65mg/ml  $680.00

HLA-DRB1 Antibody (clone LN3 + HLA-DRB/1067) LS-C390344
Mouse Monoclonal [clone LN3 + HLA-DRB/1067] (IgG) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg  $395.00
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone LN3 + HLA-DRB/1067, Azide-free) LS-C390346
Mouse Monoclonal [clone LN3 + HLA-DRB/1067] (IgG) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone LN3 + HLA-DRB/1067, Ready-to-Use) LS-C390347
Mouse Monoclonal [clone LN3 + HLA-DRB/1067] (IgG) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with HLA-DRB1 antibody (LN3 + HLA-DRB/1067).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM288) LS-C390321
Mouse Monoclonal [clone SPM288] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg  $395.00
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM288).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM288).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM288, Azide-free) LS-C390334
Mouse Monoclonal [clone SPM288] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM288).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM288).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM288, Ready-to-Use) LS-C390330
Mouse Monoclonal [clone SPM288] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM288).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM288).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM289) LS-C390322
Mouse Monoclonal [clone SPM289] (IgG2b,k) to Human HLA-DRB1
Human
Flo IF IHC IHC-P WB
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg  $395.00
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM289).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM289).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM289, Azide-free) LS-C390324
Mouse Monoclonal [clone SPM289] (IgG2b,k) to Human HLA-DRB1
Human
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM289).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM289).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM289, Ready-to-Use) LS-C390319
Mouse Monoclonal [clone SPM289] (IgG2b,k) to Human HLA-DRB1
Human
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM289).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB1 antibody (SPM289).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM423) LS-C390331
Mouse Monoclonal [clone SPM423] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
20µg 0.2mg/ml  $255.00; 100µg  $395.00
Formalin-fixed, paraffin-embedded human Histiocytoma stained with anti-HLA-DRB1 antibody (SPM423).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Histiocytoma stained with anti-HLA-DRB1 antibody (SPM423).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM423, Azide-free) LS-C390339
Mouse Monoclonal [clone SPM423] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $415.00
Formalin-fixed, paraffin-embedded human Histiocytoma stained with anti-HLA-DRB1 antibody (SPM423).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Histiocytoma stained with anti-HLA-DRB1 antibody (SPM423).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (clone SPM423, Ready-to-Use) LS-C390338
Mouse Monoclonal [clone SPM423] (IgG2b,k) to Human HLA-DRB1
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated, Ready-to-Use
7000µl  $395.00
Formalin-fixed, paraffin-embedded human Histiocytoma stained with anti-HLA-DRB1 antibody (SPM423).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Histiocytoma stained with anti-HLA-DRB1 antibody (SPM423).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA-DRB1 Antibody (aa30-58) LS-C203667
Rabbit Polyclonal to Human HLA-DRB1
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

HLA-DRB1 Antibody LS-C409239
Rabbit Polyclonal (IgG) to Human HLA-DRB1
Mouse, Human
IF IHC WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of A549 cells.
Western blot analysis of extracts of various cells.
Immunofluorescence analysis of A549 cells.
Western blot analysis of extracts of various cells.

HLA-DRB1 Antibody LS-C482862
Rabbit Polyclonal to Human HLA-DRB1
Human
ICC IF WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunofluorescent analysis of HLA-DRB1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of HLA-DRB1 expression in MCF7 (A); mouse spleen (B); mouse heart (C) whole cell lysates.
Immunofluorescent analysis of HLA-DRB1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of HLA-DRB1 expression in MCF7 (A); mouse spleen (B); mouse heart (C) whole cell lysates.
Showing 1-25 of 68 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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