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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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H2AFX / H2AX

H2A histone family, member X

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gene Name: H2A histone family, member X
Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone
Target Sequences: NM_002105 NP_002096.1 P16104

Publications (12)

1
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X. Molecular and cellular biology. 2007 27:5393-402. [PubMed:17526736] [PMC:PMC1952092] Related Antibodies: LS-C41114.
2
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL. Nature. 2007 447:725-9. [PubMed:17554309] Related Antibodies: LS-C41114.
3
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. Mochizuki K, Novatchkova M, Loidl J. Journal of cell science. 2008 121:2148-58. [PubMed:18522989] [PMC:PMC3184542] Related Antibodies: LS-C41114.
4
Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH. The American journal of pathology. 2008 173:536-44. [PubMed:18599611] [PMC:PMC2475790] Related Antibodies: LS-C41114.
5
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Loidl J, Mochizuki K. Molecular biology of the cell. 2009 20:2428-37. [PubMed:19297526] [PMC:PMC2675622] Related Antibodies: LS-C41114.
6
gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. Circulation. 2009 120:1903-9. [PubMed:19858412]
7
Tumor regression and curability of preclinical neuroblastoma models by PEGylated SN38 (EZN-2208), a novel topoisomerase I inhibitor. Pastorino F, Loi M, Sapra P, Becherini P, Cilli M, Emionite L, Ribatti D, Greenberger LM, Horak ID, Ponzoni M. Clinical cancer research : an official journal of the American Association for Cancer Research. 2010 16:4809-21. (IHC-Fr; Mouse) [PubMed:20702613]
8
Reduced NR4A gene dosage leads to mixed myelodysplastic/myeloproliferative neoplasms in mice. Ramirez-Herrick AM, Mullican SE, Sheehan AM, Conneely OM. Blood. 2011 117:2681-90. [PubMed:21205929] [PMC:PMC3062356]
9
The SET2-RPB1 interaction domain of human RECQ5 is important for transcription-associated genome stability. Li M, Xu X, Liu Y. Molecular and cellular biology. 2011 31:2090-9. [PubMed:21402780] [PMC:PMC3133350]
10
The nucleolus directly regulates p53 export and degradation. Boyd MT, Vlatkovic N, Rubbi CP. The Journal of cell biology. 2011 194:689-703. [PubMed:21893597] [PMC:PMC3171122]
11
Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells. Conomos D, Stutz MD, Hills M, Neumann AA, Bryan TM, Reddel RR, Pickett HA. The Journal of cell biology. 2012 199:893-906. (ICC; Human) [PubMed:23229897] [PMC:PMC3518223]
12
Ionizing Radiation-Induced -H2AX Activity in Whole Blood Culture and the Risk of Lung Cancer. He Y, Gong Y, Lin J, Chang DW, Gu J, Roth JA, Wu X. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2013 22:443-51. [PubMed:23300022] [PMC:PMC3601549]
more

Viewing 1-9 of 9 items
H2AFX / H2AX Protein - Recombinant H2A Histone Family, Member X By SDS-PAGE
Select
E. coli
His + GST
47.0 kDa
10µg   $285.00; 50µg   $465.00; 100µg   $620.00; 200µg   $920.00; 1mg   $2,340.00; 500µg   $1,035.00
H2AFX / H2AX Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST,N-terminus
10µg   $425.00; 25µg   $590.00
Select
293T Cells
Myc + DDK
15 kDa
20µg   $195.00
H2AFX / H2AX Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
GST,N-terminus
1mg   $1,290.00; 500µg   $830.00; 200µg   $485.00; 100µg   $355.00; 50µg   $275.00; 10µg   $245.00
H2AFX / H2AX Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His,N-terminus
1mg   $1,290.00; 500µg   $830.00; 200µg   $485.00; 100µg   $355.00; 50µg   $275.00; 10µg   $245.00
Select
HEK 293 Cells
Myc + DDK
15 kDa
100µg   $365.00
H2AFX / H2AX Protein - 12.5% SDS-PAGE of human H2AFX stained with Coomassie Blue
Select
Wheat Germ Extract
GST
41.47 kDa
2µg   $385.00
H2AFX / H2AX Protein
Select
E. coli
GST, N-terminus + His, C-terminus
43 kDa
50µg   $325.00
H2AFX / H2AX Protein
Select
HEK 293 Cells
Myc + DDK, C-terminus
15 kDa
20µg   $890.00

Viewing 1-9 of 9 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).