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FGF8

fibroblast growth factor 8 (androgen-induced)

FGF8 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.

Gene Name: fibroblast growth factor 8 (androgen-induced)
Family/Subfamily: HBGF , not assigned-HBGF
Synonyms: FGF8, AIGF, Androgen-induced growth factor, FGF-8, Fibroblast growth factor 8, HBGF-8, KAL6, HH6
Target Sequences: NM_006119 NP_006110.1

Publications (1)

1
Dynamic secretion during meiotic reentry integrates the function of the oocyte and cumulus cells. Hakan Cakmak, Federica Franciosi, A Musa Zamah, Marcelle I Cedars, Marco Conti. Proceedings of the National Academy of Sciences of the United States of America. 2016 March;113:2424-9. [Full Text Article] [PubMed:26864200] [PMC:PMC4780620]

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Proteins (11)
Over-Expression Lysate (2)
Recombinant (9)
FGF8 (11)
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6His, N-terminus (1)
Myc-DDK (Flag) (2)
Human (9)
Mouse (1)
293T Cells (1)
E. coli (8)
HEK 293 Cells (1)
Purified (4)
Low endotoxin level (8)
Biologically active (7)
Animal-free (1)
FGF8 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
25.3 kDa
20 µg/$150
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E. coli
None
Biologically active
Less than 0.2 EU/µg protein (determined by LAL method).
22.5 kDa (SDS-PAGE)
50 µg/$364; 10 µg/$278; 1 mg/$1,933
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E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
22.5kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
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E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
27.7kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
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None
Biologically active
22.4 kD
25 µg/$492
FGF8 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
25.3 kDa
100 µg/$494
FGF8 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
22.5 kDa
50 µg/$516; 10 µg/$313; 1 mg/$2,837; 500 µg/$1,987
Select
E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
~22.5 kDa
50 µg/$291; 1 mg/$1,813
FGF8 Protein - The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
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E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
50 µg/$291; 1 mg/$1,813
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
22.5 kDa
5 µg/$268; 25 µg/$349
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
5 µg/$281; 25 µg/$369
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).