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Unfortunately, the antibody (ID:145298) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



FAK / Focal Adhesion Kinase

protein tyrosine kinase 2

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Gene Name: protein tyrosine kinase 2
Family/Subfamily: Protein Kinase , Focal Adhesion Kinase
Synonyms: PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK
Target Sequences: NM_005607 NP_005598.3 Q05397

Publications (3)

1
Expression of Src and FAK in hepatocellular carcinoma and the effect of Src inhibitors on hepatocellular carcinoma in vitro. Lau GM, Lau GM, Yu GL, Gelman IH, Gutowski A, Hangauer D, Fang JW. Digestive diseases and sciences. 2009 54:1465-74. (IHC) [PubMed:18979199]
2
Neutrophil granulocytes promote the migratory activity of MDA-MB-468 human breast carcinoma cells via ICAM-1. Strell C, Lang K, Niggemann B, Zaenker KS, Entschladen F. Experimental cell research. 2010 316:138-48. (WB) [PubMed:19747913]
3
Leptin stimulates the migration of colon carcinoma cells by multiple signaling pathways. Ratke J, Entschladen F, Niggemann B, Znker KS, Lang K. Endocrine-related cancer. 2010 17:179-89. (WB) [PubMed:19952122]

Filtered By: None
Target
fak-focal-adhesion-kinase (213)
Reactivity
Human (198)
Mouse (149)
Rat (125)
Avian (6)
Bat (2)
Bovine (6)
Chicken (40)
Dog (19)
Drosophila (3)
Frog (12)
Guinea pig (3)
Hamster (10)
Horse (7)
Monkey (17)
Rabbit (7)
Rodent (7)
Xenopus (10)
Zebrafish (10)
Application
IHC (76)
IHC-P (34)
WB (181)
Flo (3)
ELISA (73)
ICC (21)
IF (38)
IP (40)
Host
rabbit (175)
mouse (38)
Isotype
IgG (124)
IgG1 (34)
IgG1,k (2)
IgG2a,k (1)
Clonality
monoclonal mc (39)
polyclonal pc (174)
Clone
10H7 (1)
10H7A6 (1)
10H7E9 (1)
1297CT261.163.145 (1)
12G4 (1)
2C3 (1)
4.47 (1)
4A8 (1)
4A9D6 (1)
4D11 (1)
4G7 (1)
Format
Agarose Conjugated (1)
AP Conjugated (9)
APC Conjugated (9)
Azide-free (2)
Biotin Conjugated (12)
FITC Conjugated (12)
HRP Conjugated (11)
PE Conjugated (9)
Unconjugated (150)
Epitope
pTyr397 (15)
pTyr861 (10)
pTyr925 (9)
aa426-453 (8)
pTyr576 (7)
Internal (6)
pTyr407 (6)
C-Terminus (4)
aa198-246 (4)
pTyr577 (4)
aa798-1041 (3)
pSer843 (3)
aa1-423 (2)
aa340-420 (2)
aa520-600 (2)
aa706-1052 (2)
pSer910 (2)
pTyr576/Tyr577 (2)
Arg919 (1)
Glu393 (1)
Ile401 (1)
Ile855 (1)
Kinase Domain (1)
Pro906 (1)
Tyr570 (1)
aa1-128 (1)
aa1002-1052 (1)
aa1024-1052 (1)
aa103-553 (1)
aa1036-1052 (1)
aa325-375 (1)
aa350-430 (1)
aa364-413 (1)
aa373-422 (1)
aa542-591 (1)
aa543-592 (1)
aa574-578 (1)
aa670-750 (1)
aa710 (1)
aa748-1053 (1)
aa780-860 (1)
aa787-836 (1)
aa800-880 (1)
aa809-858 (1)
aa821-848 (1)
aa828-877 (1)
aa850-930 (1)
aa860-940 (1)
aa876-925 (1)
aa892-941 (1)
pSer576 (1)
pSer732 (1)
pTyr576/577 (1)
Publications
No (213)
Showing 1-25 out of 213 results
Anti-FAK / Focal Adhesion Kinase Antibody (Internal) IHC-plus™ LS-A3392
Immunohistochemistry Image
Anti-FAK antibody LS-A3392 IHC of human ductal and lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Chicken, Bovine, Rat, Hamster, Horse, Bat, Rabbit, Mouse, Dog, Human, Monkey
IHC IHC-P
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa1-423, clone 4.47) IHC-plus™ LS-B212
Anti-FAK antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B212 concentration 20 ug/ml.

Mouse Monoclonal [clone 4.47] (IgG1) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA ICC IHC IHC-P IP WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (AP) LS-C507958

Mouse Monoclonal (IgG1) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA ICC IHC IP WB
AP Conjugated
Anti-FAK / Focal Adhesion Kinase Antibody (APC) LS-C516702

Mouse Monoclonal (IgG1) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA ICC IHC IP WB
APC Conjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa706-1052) IHC-plus™ LS-B7154
Anti-FAK antibody IHC of human colon, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7154 dilution 1:200.
Western blot Image

Rabbit Polyclonal (IgG1) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa821-848) LS-C162285
Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of PTK2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Human
Flo IF IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody LS-C497784

Rabbit Monoclonal to Human FAK / Focal Adhesion Kinase
Human
Flo IF IHC IP WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (C-Terminus) IHC-plus™ LS-A2953
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK antibody LS-A2953 IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Human, Monkey
IHC IHC-P
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (phospho-Tyr925) LS-C177998
IHC of p-FAK (Y925) pAb in paraffin-embedded human brain tissue.
Western blot of p-FAK (Y925) pAb in extracts from HepG2 cells.

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody LS-C15112

Mouse Monoclonal (IgG1) to Chicken FAK / Focal Adhesion Kinase
Chicken, Rodent, Human
IF IP
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (phospho-Tyr577) LS-C15122

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Human
ELISA WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (clone 4G7) LS-C174728
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTK2 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody.

Mouse Monoclonal [clone 4G7] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
IF IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (clone 4D11) LS-C174723
IHC of paraffin-embedded Human Kidney tissue using anti-PTK2 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody.

Mouse Monoclonal [clone 4D11] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
IF IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (clone 4A8) LS-C338066
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Mouse Monoclonal [clone 4A8] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
IF IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (clone 10H7) LS-C169201
IHC of paraffin-embedded cervices tumor using FAK mouse monoclonal antibody with DAB staining
Immunofluorescence of B16 cells using FAK mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal [clone 10H7] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
ELISA IF IHC WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (Internal) LS-C352764
Immunohistochemical analysis of Focal Adhesion Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Focal Adhesion Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Chicken, Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (C-Terminus) LS-C368815
Immunohistochemical analysis of Focal Adhesion Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Focal Adhesion Kinase staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Human
ICC IF IHC IHC-P IP WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (phospho-Tyr576) IHC-plus™ LS-B2522
Anti-FAK antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2522 dilution 1:100.

Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Frog, Chicken, Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa340-420) LS-C383468
Western blot of FAK antibody

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa340-420) LS-C383456
Western blot of FAK antibody

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa800-880) LS-C383466
Western blot of FAK antibody

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa860-940) LS-C383464
Western blot of FAK antibody
Western blot of FAK antibody

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (phospho-Ser910) LS-C198949
IHC of paraffin-embedded human brain, using FAK (Phospho-Ser910) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HepG2 cells, using FAK (Phospho-Ser910) Antibody. The sample on the right was incubated with synthetic peptide.

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA IF IHC IHC-P
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody LS-C330771
Western blot analysis of extracts of MCF7 cells.

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IF IHC IP WB
Unconjugated
Anti-FAK / Focal Adhesion Kinase Antibody (aa373-422) IHC-plus™ LS-B12417
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of extracts from HeLa cells, treated with EGF 200 ng/ml 30', using FAK (Ab-407) Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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