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F9 / Factor IX

coagulation factor IX

F9 / Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Gene Name: coagulation factor IX
Family/Subfamily: Protease , Serine S1
Synonyms: F9, Christmas Disease, Coagulation factor IX, Factor IX Deficiency, FIX, HEMB, F9 p22, Factor 9, Factor IX, Factor IX F9, FIX F9, Haemophilia B, THPH8, Christmas factor, Hemophilia B, PTC, Serine protease
Target Sequences: NM_000133 NP_000124.1 P00740

Publications (4)

1
Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells. Li SJ, Luo Y, Zhang LM, Yang W, Zhang GG. Molecular medicine reports. 2017 March;15:1313-1318. [Full Text Article] [PubMed:28112377] [PMC:PMC5367337]
2
Preclinical development of plant-based oral immune modulatory therapy for haemophilia B. Aparajitha Srinivasan, Roland W Herzog, Imran Khan, Alexandra Sherman, Thais Bertolini, Tung Wynn, Henry Daniell. Plant biotechnology journal. 2021 Oct;;19:1952-1966. [Full Text Article] [PubMed:33949086] [PMC:PMC8486253]
3
A novel next-generation FVIIIa mimetic, Mim8, has a favorable safety profile and displays potent pharmacodynamic effects: Results from safety studies in cynomolgus monkeys. Brian Lauritzen, Mads Bjelke, Olle Björkdahl, Esther Bloem, Kevin Keane, Marianne Kjalke, Marie Rossen, Solvej Lund Lippert, Karin Nana Weldingh, Mikala Skydsgaard, Stine Kjellev. Journal of thrombosis and haemostasis : JTH. 2022 June;20:1312-1324. [Full Text Article] [PubMed:35191180] [PMC:PMC9314625] Related Antibodies: LS-B7226.
4
A lipoprotein receptor cluster IV mutant preferentially binds amyloid- beta and regulates its clearance from the mouse brain. Sagare AP, Bell RD, Srivastava A, Sengillo JD, Singh I, Nishida Y, Chow N, Zlokovic BV. The Journal of biological chemistry. 2013 May;288:15154-66. [Full Text Article] [PubMed:23580652] [PMC:PMC3663536] Related Antibodies: LS-C23381.
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Proteins (14)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (2)
Recombinant (11)
F9 / Factor IX (14)
No (14)
6His, C-terminus (2)
GST (1)
GST, N-terminus (1)
His (5)
His, N-Terminal (1)
His,C-terminus (1)
His-GST (1)
Myc-DDK (Flag) (2)
Bovine (1)
Human (8)
Mouse (2)
Rabbit (1)
Rat (1)
293T Cells (1)
E. coli (5)
HEK 293 Cells (3)
Human Cells (2)
Mammalian Cells (1)
Wheat Germ Extract (2)
Purified (1)
Low endotoxin level (4)
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HEK 293 Cells
His, N-Terminal
~47 kDa
200 µl/$283
F9 / Factor IX Protein - 12.5% SDS-PAGE of human F9 stained with Coomassie Blue
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Wheat Germ Extract
GST
78.2 kDa
10 µg/$479; 25 µg/$670
F9 / Factor IX Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
51.78 kDa
20 µg/$215
F9 / Factor IX Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
F9 / Factor IX Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
51.78 kDa
100 µg/$710
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HEK 293 Cells
His,C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
51.3 kDa
20 µg/$600
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Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
49.8 kDa
10 µg/$368; 50 µg/$479
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
49.8kD
10 µg/$281; 50 µg/$395; 500 µg/$1,585; 1 mg/$2,157
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Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
50 kDa
50 µg/$721
F9 / Factor IX Protein - Recombinant Coagulation Factor IX By SDS-PAGE
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E. coli
His
26.5 kDa
10 µg/$268; 50 µg/$362; 100 µg/$577; 200 µg/$722; 1 mg/$1,755; 500 µg/$1,325; 5 mg/$2,708; 2 mg/$1,934
F9 / Factor IX Protein - Recombinant Coagulation Factor IX By SDS-PAGE
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E. coli
His
26.5 kDa
10 µg/$284; 50 µg/$462; 100 µg/$737; 200 µg/$922; 1 mg/$2,042; 500 µg/$1,577; 5 mg/$3,221; 2 mg/$2,231
F9 / Factor IX Protein - Recombinant Coagulation Factor IX By SDS-PAGE
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E. coli
His
17.7 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
F9 / Factor IX Protein - Recombinant Coagulation Factor IX By SDS-PAGE
Select
E. coli
His-GST
48.7 kDa
10 µg/$289; 50 µg/$490; 100 µg/$782; 200 µg/$977; 1 mg/$2,114; 500 µg/$1,640; 5 mg/$3,417; 2 mg/$2,304
F9 / Factor IX Protein - Recombinant Coagulation Factor IX By SDS-PAGE
Select
E. coli
His
24.1 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).