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EN2

engrailed homeobox 2

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Gene Name: engrailed homeobox 2
Synonyms: EN2, AUTS10, Engrailed homolog 2, Engrailed homeobox 2, Homeobox protein engrailed-2, Engrailed-2, Homeobox protein en-2, Hu-En-2
Target Sequences: NM_001427 NP_001418.2 P19622

Publications (1)

1
Engrailed-2 (EN2) - a novel biomarker in epithelial ovarian cancer. McGrath SE, Annels N, Madhuri TK, Tailor A, Butler-Manuel SA, Morgan R, Pandha H, Michael A. BMC cancer. 2018 October;18:943. [Full Text Article] [PubMed:30285763] [PMC:PMC6171236] Related Antibodies: LS-B3477.

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Products
Antibodies (26)
Primary (26)
EN2 (26)
Human (26)
Mouse (13)
Rat (4)
Chimpanzee (3)
Gibbon (3)
Guinea pig (3)
Hamster (1)
Horse (3)
Monkey (4)
Pig (4)
IHC (7)
IHC-P (6)
WB (23)
Flo (2)
ELISA (18)
IF (2)
Peptide-ELISA (1)
rabbit (24)
mouse (1)
goat (1)
Product Group
IHCPlus (2)
IgG (21)
IgG2a,k (1)
monoclonal mc (1)
polyclonal pc (25)
1E1 (1)
APC Conjugated (2)
Azide-free (2)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (10)
aa243-271 (15)
C-Terminus (4)
aa320-333 (1)
No (25)
Yes (1)
EN2 Antibody - Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EN2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
EN2 Antibody - Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EN2 Rabbit anti-Human Polyclonal (C-Terminus) (HRP) Antibody
Mouse, Guinea pig, Rat, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
EN2 Antibody - Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EN2 Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody
Mouse, Guinea pig, Rat, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
EN2 Antibody - Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EN2 Rabbit anti-Human Polyclonal (C-Terminus) (FITC) Antibody
Mouse, Guinea pig, Rat, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
EN2 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
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EN2 Rabbit anti-Human Polyclonal (aa243-271) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
200 µl/$440
EN2 Antibody - Fluorescent confocal image of HeLa cell stained with EN2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
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EN2 Rabbit anti-Human Polyclonal (aa243-271) Antibody
Mouse, Human
IF, WB
Unconjugated
400 µl/$393
EN2 Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using EN2 Antibody at dilution of 1:100
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EN2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
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EN2 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
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EN2 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
EN2 Antibody - Detection limit for recombinant GST tagged EN2 is 3 ng/ml as a capture antibody.
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EN2 Mouse anti-Human Monoclonal (1E1) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
EN2 Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with EN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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EN2 Rabbit anti-Human Polyclonal (aa243-271) Antibody
Mouse, Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
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EN2 Rabbit anti-Human Polyclonal (aa243-271) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
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EN2 Rabbit anti-Human Polyclonal (aa243-271) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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EN2 Rabbit anti-Human Polyclonal (aa243-271) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
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EN2 Rabbit anti-Human Polyclonal (aa243-271) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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EN2 Rabbit anti-Human Polyclonal (aa243-271) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) Antibody
Mouse, Human
ELISA, WB
Unconjugated
200 µl/$734
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) (Biotin) Antibody
Mouse, Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) (FITC) Antibody
Mouse, Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) (APC) Antibody
Mouse, Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) (PE) Antibody
Mouse, Human
ELISA, WB
PE Conjugated
200 µl/$952
Select
EN2 Rabbit anti-Human Polyclonal (aa243-271) (Azide-free) (HRP) Antibody
Mouse, Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
EN2 Antibody - Western blot analysis of Jurkat cell lysate  using EN2 Polyclonal Antibody at dilution of 1:400
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EN2 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
EN2 Antibody - Antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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EN2 Goat anti-Human Polyclonal (aa320-333) Antibody
Mouse, Rat, Hamster, Pig, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100 µg/$503
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).