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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF5B / IF2

eukaryotic translation initiation factor 5B

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Gene Name: eukaryotic translation initiation factor 5B
Synonyms: EIF5B, EIF-5B, IF2, KIAA0741
Target Sequences: NM_015904 NP_056988.3 O60841

Filtered By: None
Target
EIF5B / IF2 (21)
Reactivity
Human (21)
Mouse (5)
Rat (5)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Horse (4)
Monkey (5)
Orangutan (3)
Application
IHC (5)
IHC-P (2)
WB (14)
ELISA (9)
IF (3)
IP (2)
Peptide-ELISA (1)
Host
rabbit (13)
mouse (8)
Isotype
IgG (8)
IgG2a,k (7)
Clonality
monoclonal mc (7)
polyclonal pc (14)
Format
AP Conjugated (1)
APC Conjugated (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (12)
Epitope
aa557-606 (4)
1020-1100 aa, C-terminal (1)
C-Terminus (1)
His1086 (1)
aa1051-1100 (1)
aa1121-1218 (1)
aa450-500 (1)
aa75-125 (1)
Publications
No (21)
Showing 1-21 of 21 results

EIF5B / IF2 Antibody LS-C665330
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Human
ELISA IF IHC WB
Unconjugated
100µg  $355.00

EIF5B / IF2 Antibody LS-C334362
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using EIF5B antibody.
Western blot analysis of extracts of various cell lines, using EIF5B antibody.

EIF5B / IF2 Antibody (C-Terminus) LS-C353185
Rabbit Polyclonal to Human EIF5B / IF2
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of EIF5B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of EIF5B expression in A549 (A); Jurkat (B) whole cell lysates.
Immunohistochemical analysis of EIF5B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of EIF5B expression in A549 (A); Jurkat (B) whole cell lysates.

EIF5B / IF2 Antibody (1020-1100 aa, C-terminal) LS-C383308
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00

EIF5B / IF2 Antibody (His1086) LS-C177374
Rabbit Polyclonal to Human EIF5B / IF2
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of eIF5B (H1086) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of eIF5B (H1086) pAb in extracts from Jurkat cells.
IHC of eIF5B (H1086) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of eIF5B (H1086) pAb in extracts from Jurkat cells.

EIF5B / IF2 Antibody LS-C307377
Mouse Polyclonal (IgG) to Human EIF5B / IF2
Human
IF WB
Unconjugated
50µg  $560.00

EIF5B / IF2 Antibody (aa75-125) LS-C288523
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Human
IP WB
Unconjugated
100µl 0.2mg/ml  $395.00

EIF5B / IF2 Antibody (aa1051-1100) LS-C119970
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Mouse, Rat, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from Jurkat, HepG2, and A549 cells, using EIF5B Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat, HepG2, and A549 cells, using EIF5B Antibody. The lane on the right is blocked with the synthesized peptide.

EIF5B / IF2 Antibody (aa1121-1218) LS-C307378
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
Unconjugated
100µg  $560.00

EIF5B / IF2 Antibody (HRP) LS-C541956
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
HRP Conjugated
100µl  $710.00

EIF5B / IF2 Antibody (Biotin) LS-C521825
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
Biotin Conjugated
100µl  $710.00

EIF5B / IF2 Antibody (FITC) LS-C531869
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
FITC Conjugated
100µl  $710.00

EIF5B / IF2 Antibody (AP) LS-C503900
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
AP Conjugated
100µl  $710.00

EIF5B / IF2 Antibody (APC) LS-C513610
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
APC Conjugated
100µl  $710.00

EIF5B / IF2 Antibody (PE) LS-C650070
Mouse Monoclonal (IgG2a,k) to Human EIF5B / IF2
Human
ELISA
PE Conjugated
100µl  $710.00

EIF5B / IF2 Antibody (aa450-500) LS-C288522
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Human, Monkey
WB
Unconjugated
100µl 0.2mg/ml  $395.00

EIF5B / IF2 Antibody (aa557-606) LS-C145468
Rabbit Polyclonal to Human EIF5B / IF2
Dog, Horse, Human, Monkey
WB
Unconjugated
50µg  $365.00
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF5B / IF2 Antibody (aa557-606, HRP) LS-C468885
Rabbit Polyclonal to Human EIF5B / IF2
Dog, Horse, Orangutan, Gibbon, Human, Monkey, Chimpanzee
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF5B / IF2 Antibody (aa557-606, Biotin) LS-C468883
Rabbit Polyclonal to Human EIF5B / IF2
Dog, Horse, Orangutan, Gibbon, Human, Monkey, Chimpanzee
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF5B / IF2 Antibody (aa557-606, FITC) LS-C468884
Rabbit Polyclonal to Human EIF5B / IF2
Dog, Horse, Orangutan, Gibbon, Human, Monkey, Chimpanzee
WB
FITC Conjugated
100µl 1mg/ml  $385.00
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF5B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF5B / IF2 Antibody LS-C750095
Rabbit Polyclonal (IgG) to Human EIF5B / IF2
Human
WB
Unconjugated
50µl  $235.00
Western blot analysis of extracts of various cell lines, using EIF5B antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
Western blot analysis of extracts of various cell lines, using EIF5B antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
Showing 1-21 of 21 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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