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EIF3D

eukaryotic translation initiation factor 3, subunit D

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene Name: eukaryotic translation initiation factor 3, subunit D
Synonyms: EIF3D, EIF3-zeta, EIF3 p66, EIF3-p66, EIF-3-zeta, EIF3S7
Target Sequences: NM_003753 NP_003744.1 O15371

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Products
Antibodies (31)
Primary (31)
EIF3D (31)
Human (30)
Mouse (15)
Rat (10)
Bat (3)
Bovine (7)
Chicken (6)
Chimpanzee (6)
Dog (6)
Fish (1)
Gibbon (6)
Guinea pig (6)
Horse (6)
Macaque (1)
Monkey (8)
Orangutan (6)
Pig (6)
Rabbit (6)
Xenopus (3)
Zebrafish (7)
IHC (11)
IHC-P (4)
WB (27)
ELISA (8)
ICC (1)
IF (4)
IP (1)
Peptide-ELISA (2)
rabbit (26)
mouse (5)
Product Group
IHCPlus (1)
IgG (14)
IgG1 (5)
monoclonal mc (5)
polyclonal pc (26)
OTI14D6 (2)
OTI1G10 (2)
Biotin Conjugated (3)
Carrier-free (2)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (22)
N-Terminus (4)
aa124-173 (4)
70-150 aa, Internal (1)
Arg135 (1)
Internal (1)
aa101-150 (1)
aa150-200 (1)
aa200-250 (1)
aa500-550 (1)
No (31)
EIF3D Antibody - Anti-EIF3D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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EIF3D Rabbit anti-Human Polyclonal (aa101-150) Antibody
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$440
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EIF3D Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$341
EIF3D Antibody - 1:100 staining human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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EIF3D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
EIF3D Antibody - Western blot analysis of extracts of Mouse brain cell lines.
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EIF3D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
EIF3D Antibody - IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-EIF3D mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
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EIF3D Mouse anti-Human Monoclonal (OTI14D6) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$434
EIF3D Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EIF3D mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)
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EIF3D Mouse anti-Human Monoclonal (Carrier-free) (OTI14D6) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
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EIF3D Mouse anti-Human Monoclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$351
EIF3D Antibody - Immunofluorescence staining of EIF3D in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EIF3D polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
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EIF3D Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, WB
Unconjugated
100 µl/$412
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EIF3D Rabbit anti-Human Polyclonal (70-150 aa, Internal) Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
EIF3D Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EIF3D antibody.
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EIF3D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
100 µg/$357
EIF3D Antibody - IHC of eIF3 (R135) pAb in paraffin-embedded human breast carcinoma tissue.
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EIF3D Rabbit anti-Human Polyclonal (Arg135) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
EIF3D Antibody - Immunohistochemical analysis of EIF3D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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EIF3D Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Bovine, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
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EIF3D Rabbit anti-Human Polyclonal (aa150-200) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
EIF3D Antibody - IHC image of EIF3D Antibody diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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EIF3D Rabbit anti-Human Polyclonal Antibody
Human
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
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EIF3D Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
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EIF3D Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
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EIF3D Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
EIF3D Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY EIF3D. (Right lane) cDNA for 48 hrs and lysed
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EIF3D Mouse anti-Human Monoclonal (OTI1G10) Antibody
Human
WB
Unconjugated
100 µl/$434
EIF3D Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY EIF3D. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF3D. (1:2000)
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EIF3D Mouse anti-Human Monoclonal (Carrier-free) (OTI1G10) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
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EIF3D Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
EIF3D Antibody - EIF3D antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EIF3D Rabbit anti-Human Polyclonal (aa124-173) Antibody
Human
WB
Unconjugated
100 µl/$424
EIF3D Antibody - EIF3D antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EIF3D Rabbit anti-Human Polyclonal (aa124-173) (Biotin) Antibody
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon, Orangutan, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
EIF3D Antibody - Western blot of eIF3D in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using Peptide-affinity Purified Polyclonal Antibody to eIF3D at 0.01 ug/ml, 0.01 ug/ml, 0.005 ug/ml and 0.01 ug/ml, respectively.
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EIF3D Rabbit anti-Human Polyclonal (aa500-550) Antibody
Fish, Zebrafish, Mouse, Rat, Human, Monkey, Macaque
WB
Unconjugated
100 µg/$386
EIF3D Antibody - Detection of Human eIF3D/EIF3S7 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3D/EIF3S7 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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EIF3D Rabbit anti-Human Polyclonal (aa200-250) Antibody
Human
WB
Unconjugated
10 µl/$295; 100 µl/$514
EIF3D Antibody - All lanes: Anti-EIF3D Antibody at 1:1000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 64 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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EIF3D Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$386
Viewing 1-25 of 31 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).