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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF3D

eukaryotic translation initiation factor 3, subunit D

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene Name: eukaryotic translation initiation factor 3, subunit D
Synonyms: EIF3D, EIF3-zeta, EIF3 p66, EIF3-p66, EIF-3-zeta, EIF3S7
Target Sequences: NM_003753 NP_003744.1 O15371

Filtered by: None
Target
EIF3D (29)
Reactivity
Human (29)
Mouse (16)
Rat (11)
Bat (4)
Bovine (9)
Chicken (8)
Chimpanzee (6)
Dog (8)
Fish (1)
Gibbon (6)
Guinea pig (8)
Horse (8)
Macaque (1)
Monkey (10)
Orangutan (6)
Pig (8)
Rabbit (8)
Xenopus (4)
Zebrafish (9)
Application
IHC (10)
IHC-P (4)
WB (25)
ELISA (8)
IF (3)
IP (1)
Peptide-ELISA (1)
Host
rabbit (24)
mouse (5)
Isotype
IgG (12)
IgG1 (5)
Clonality
monoclonal mc (5)
polyclonal pc (24)
Clone
OTI14D6 (2)
OTI1G10 (2)
Format
Biotin Conjugated (3)
Carrier-free (2)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (20)
Epitope
N-Terminus (4)
aa124-173 (4)
70-150 aa, Internal (1)
Arg135 (1)
Internal (1)
aa101-150 (1)
aa150-200 (1)
aa200-250 (1)
aa500-550 (1)
Publications
No (29)
Showing 1-25 of 29 results

IHC-plus™ EIF3D Antibody (aa101-150) LS-B5687
Rabbit Polyclonal (IgG) to Human EIF3D
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-EIF3D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from NIH/3T3 cells, using EIF3D Antibody. The lane on the right is blocked with the synthesized peptide.

EIF3D Antibody LS-C805054
Rabbit Polyclonal (IgG) to Human EIF3D
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

EIF3D Antibody LS-C665318
Rabbit Polyclonal (IgG) to Human EIF3D
Human
ELISA, IF, IHC, WB
Unconjugated
100µg  $355.00

EIF3D Antibody LS-C334408
Rabbit Polyclonal (IgG) to Human EIF3D
Mouse, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of Mouse brain cell lines.

EIF3D Antibody LS-C665319
Mouse Monoclonal (IgG1) to Human EIF3D
Human
ELISA, IHC, WB
Unconjugated
100µg  $355.00

EIF3D Antibody (clone OTI14D6) LS-C340457
Mouse Monoclonal [clone OTI14D6] (IgG1) to Human EIF3D
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-EIF3D mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EIF3D mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150).

EIF3D Antibody (clone OTI14D6, Carrier-free) LS-C791511
Mouse Monoclonal [clone OTI14D6] (IgG1) to Human EIF3D
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EIF3D mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EIF3D mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)

EIF3D Antibody (70-150 aa, Internal) LS-C383302
Rabbit Polyclonal (IgG) to Human EIF3D
Mouse, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

EIF3D Antibody (Arg135) LS-C177835
Rabbit Polyclonal to Human EIF3D
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of eIF3 (R135) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of eIF3 (R135) pAb in extracts from 3T3 cells.

EIF3D Antibody (Internal) LS-C368695
Rabbit Polyclonal to Human EIF3D
Mouse, Bovine, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of EIF3D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of EIF3D expression in HeLa (A); NIH3T3 (B) whole cell lysates.

EIF3D Antibody (aa150-200) LS-C288539
Rabbit Polyclonal (IgG) to Human EIF3D
Human
IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

EIF3D Antibody LS-C395849
Rabbit Polyclonal (IgG) to Human EIF3D
Human
ELISA
Unconjugated
50µg  $285.00; 100µg  $385.00
IHC image of EIF3D Antibody diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of EIF3D Antibody diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

EIF3D Antibody (HRP) LS-C395847
Rabbit Polyclonal (IgG) to Human EIF3D
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

EIF3D Antibody (Biotin) LS-C395839
Rabbit Polyclonal (IgG) to Human EIF3D
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

EIF3D Antibody (FITC) LS-C395870
Rabbit Polyclonal (IgG) to Human EIF3D
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

EIF3D Antibody (clone OTI1G10) LS-C794279
Mouse Monoclonal [clone OTI1G10] (IgG1) to Human EIF3D
Human
WB
Unconjugated
100µl 1mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY EIF3D. (Right lane) cDNA for 48 hrs and lysed
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY EIF3D. (Right lane) cDNA for 48 hrs and lysed

EIF3D Antibody (clone OTI1G10, Carrier-free) LS-C798500
Mouse Monoclonal [clone OTI1G10] (IgG1) to Human EIF3D
Human
WB
Unconjugated, Carrier-free
100µg  $395.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY EIF3D. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF3D. (1:2000)

EIF3D Antibody LS-C766289
Rabbit Polyclonal (IgG) to Human EIF3D
Mouse, Rat, Human
WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

EIF3D Antibody (aa124-173) LS-C146008
Rabbit Polyclonal to Human EIF3D
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
EIF3D antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3D Antibody (aa124-173, HRP) LS-C469772
Rabbit Polyclonal to Human EIF3D
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon, Orangutan, Chimpanzee
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
EIF3D antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3D Antibody (aa124-173, Biotin) LS-C469770
Rabbit Polyclonal to Human EIF3D
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon, Orangutan, Chimpanzee
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
EIF3D antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3D Antibody (aa124-173, FITC) LS-C469771
Rabbit Polyclonal to Human EIF3D
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon, Orangutan, Chimpanzee
WB
FITC Conjugated
100µl 1mg/ml  $385.00
EIF3D antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3D Antibody (aa200-250) LS-C288540
Rabbit Polyclonal (IgG) to Human EIF3D
Human
WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human eIF3D/EIF3S7 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3D/EIF3S7 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

EIF3D Antibody (aa500-550) LS-C148514
Rabbit Polyclonal to Human EIF3D
Fish, Zebrafish, Mouse, Rat, Human, Monkey, Macaque
WB
Unconjugated
100µg  $335.00
Western blot of eIF3D in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using Peptide-affinity Purified Polyclonal Antibody to eIF3D at 0.01 ug/ml, 0.01 ug/ml, 0.005 ug/ml and 0.01 ug/ml, respectively.

EIF3D Antibody LS-C394159
Rabbit Polyclonal to Human EIF3D
Human
WB
Unconjugated
100µl  $325.00
All lanes: Anti-EIF3D Antibody at 1:1000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 64 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Showing 1-25 of 29 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).