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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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E2F5

E2F transcription factor 5, p130-binding

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Gene Name: E2F transcription factor 5, p130-binding
Synonyms: E2F5, Transcription factor E2F5, E2F-5
Target Sequences: NM_001951 NP_001942.2 Q15329

Filtered By: None
Target
E2F5 (28)
Reactivity
Human (28)
Mouse (13)
Rat (13)
Dog (4)
Gibbon (6)
Guinea pig (4)
Horse (8)
Monkey (4)
Pig (4)
Rabbit (4)
Zebrafish (4)
Application
IHC (4)
IHC-P (4)
WB (25)
Flo (1)
DB (2)
ELISA (12)
ICC (2)
IF (2)
Peptide-ELISA (1)
Host
rabbit (25)
mouse (3)
Isotype
IgG (17)
IgG1 (3)
Clonality
monoclonal mc (3)
polyclonal pc (25)
Clone
220D1a (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (13)
Epitope
aa66-93 (8)
aa134-183 (4)
aa241-290 (4)
Internal (1)
Leu142 (1)
aa93-142 (1)
Publications
No (28)
Showing 1-25 of 28 results

E2F5 Antibody (aa93-142) IHC-plus™ LS-B11416
Rabbit Polyclonal (IgG) to Human E2F5
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-E2F5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Western blot analysis of lysate from HeLa cells, using E2F-5 antibody.
Anti-E2F5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Western blot analysis of lysate from HeLa cells, using E2F-5 antibody.

E2F5 Antibody (aa66-93) LS-C167599
Rabbit Polyclonal to Human E2F5
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of E2F5 Antibody in HepG2 cell line lysates (35 ug/lane). E2F5 (arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of E2F5 Antibody in HepG2 cell line lysates (35 ug/lane). E2F5 (arrow) was detected using the purified antibody.

E2F5 Antibody LS-C393385
Rabbit Polyclonal to Human E2F5
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $305.00

E2F5 Antibody (clone 220D1a) LS-C342625
Mouse Monoclonal [clone 220D1a] (IgG1) to Human E2F5
Human
DB ICC IF WB
Unconjugated
100µg 0.1mg/ml  $375.00
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-E2F5 mAb.
Western blot analysis of immunized recombinant protein, using anti-E2F5 monoclonal antibody.
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-E2F5 mAb.
Western blot analysis of immunized recombinant protein, using anti-E2F5 monoclonal antibody.

E2F5 Antibody LS-C680410
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, Rat liver tissue, Mouse liver tissue All Lanes: E2F5 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 21 KDa Observed band size: 38 KDa
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, Rat liver tissue, Mouse liver tissue All Lanes: E2F5 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 21 KDa Observed band size: 38 KDa

E2F5 Antibody (HRP) LS-C680408
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

E2F5 Antibody (Biotin) LS-C680409
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

E2F5 Antibody (FITC) LS-C680407
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

E2F5 Antibody (clone 220D1a) LS-C184590
Mouse Monoclonal [clone 220D1a] (IgG1) to Human E2F5
Human
DB ICC WB
Unconjugated
50µg 0.1mg/ml  $325.00
Western blot of immunized recombinant protein using E2F5 antibody.
Western blot of immunized recombinant protein using E2F5 antibody.

E2F5 Antibody (aa66-93) LS-C327249
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
Unconjugated
200µl 0.25mg/ml  $530.00

E2F5 Antibody (aa66-93, Biotin) LS-C277015
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

E2F5 Antibody (aa66-93, FITC) LS-C277016
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
FITC Conjugated
200µl  $680.00

E2F5 Antibody (aa66-93, AP) LS-C277012
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
AP Conjugated
200µl  $680.00

E2F5 Antibody (aa66-93, Azide-free, HRP) LS-C277017
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

E2F5 Antibody (aa66-93, PE) LS-C277018
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
PE Conjugated
200µl  $680.00

E2F5 Antibody (aa66-93, APC) LS-C277013
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA WB
APC Conjugated
200µl  $680.00

E2F5 Antibody (Internal) LS-C383234
Rabbit Polyclonal (IgG) to Human E2F5
Mouse, Rat, Human
ELISA WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of E2F-5 antibody
Western blot of E2F-5 antibody

E2F5 Antibody LS-C65138
Mouse Monoclonal (IgG1) to Human E2F5
Human
WB
Unconjugated
100µg  $545.00

E2F5 Antibody (aa241-290) LS-C146437
Rabbit Polyclonal to Mouse E2F5
Mouse, Rat, Horse, Human
WB
Unconjugated
50µg  $365.00
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

E2F5 Antibody (aa241-290, HRP) LS-C464359
Rabbit Polyclonal to Mouse E2F5
Mouse, Rat, Horse, Gibbon, Human
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

E2F5 Antibody (aa241-290, Biotin) LS-C464357
Rabbit Polyclonal to Mouse E2F5
Mouse, Rat, Horse, Gibbon, Human
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

E2F5 Antibody (aa241-290, FITC) LS-C464358
Rabbit Polyclonal to Mouse E2F5
Mouse, Rat, Horse, Gibbon, Human
WB
FITC Conjugated
100µl 1mg/ml  $385.00
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

E2F5 Antibody LS-C416958
Rabbit Polyclonal to Human E2F5
Mouse, Rat, Human
WB
Unconjugated
100µg 1mg/ml  $305.00

E2F5 Antibody (aa134-183) LS-C30420
Rabbit Polyclonal (IgG) to Human E2F5
Zebrafish, Pig, Horse, Rat, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
50µg  $365.00
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

E2F5 Antibody (Leu142) LS-C177561
Rabbit Polyclonal to Human E2F5
Mouse, Rat, Human
WB
Unconjugated
100µl  $345.00
Western blot of E2F-5 (L142) pAb in extracts from HeLa cells.
Western blot of E2F-5 (L142) pAb in extracts from HeLa cells.
Showing 1-25 of 28 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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