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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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E2F5

E2F transcription factor 5, p130-binding

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Gene Name: E2F transcription factor 5, p130-binding
Synonyms: E2F5, Transcription factor E2F5, E2F-5
Target Sequences: NM_001951 NP_001942.2 Q15329

Viewing 1-25 of 36 items
E2F5 Antibody - Anti-E2F5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Select
Rabbit Polyclonal (IgG) to Human E2F5
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
E2F5 Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Rabbit Polyclonal to Human E2F5
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
E2F5 Antibody - Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-E2F5 mAb.
Select
Mouse Monoclonal [clone 220D1a] (IgG1) to Human E2F5
Human
DB, ICC, IF, WB
Unconjugated
100µg   100µg/ml   $415.00
Select
Rabbit Polyclonal to Human E2F5
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl   $325.00
E2F5 Antibody - Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
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Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
E2F5 Antibody - Western blot of immunized recombinant protein using E2F5 antibody.
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Mouse Monoclonal [clone 220D1a] (IgG1) to Human E2F5
Human
DB, ICC, WB
Unconjugated
100µg   100µg/ml   $530.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human E2F5
Human
ELISA, WB
APC Conjugated
200µl   $740.00
E2F5 Antibody - Western blot of E2F-5 antibody
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Rabbit Polyclonal (IgG) to Human E2F5
Mouse, Rat, Human
ELISA, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
E2F5 Antibody - Western blot analysis of E2F5 expression in HeLa cells. The lane on the left is treated with the antigen-specific peptide.
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Rabbit Polyclonal (IgG) to Human E2F5
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
E2F5 Antibody - Anti-E2F5 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 30 kDa.
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Rabbit Polyclonal (IgG) to Human E2F5
Human
IP, WB
Unconjugated, Preservative Free
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Select
Mouse Monoclonal (IgG1) to Human E2F5
Human
WB
Unconjugated
100µg   $590.00
E2F5 Antibody - E2F5 antibody Western Blot of Mouse Pancreas . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Mouse E2F5
Mouse, Rat, Horse, Human
WB
Unconjugated
100µl   0.5mg/ml   $365.00
E2F5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY E2F5. (Right lane) cDNA for 48 hrs and lysed
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Mouse Monoclonal [clone OTI3B7] (IgG1) to Human E2F5
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
E2F5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY E2F5. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2F5. (1:2000)
Select
Mouse Monoclonal [clone OTI3B7] (IgG1) to Human E2F5
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
E2F5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY E2F5. (Right lane) cDNA for 48 hrs and lysed
Select
Mouse Monoclonal [clone OTI1B8] (IgG1) to Human E2F5
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
E2F5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY E2F5. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2F5. (1:2000)
Select
Mouse Monoclonal [clone OTI1B8] (IgG1) to Human E2F5
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
E2F5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY E2F5. (Right lane) cDNA for 48 hrs and lysed
Select
Mouse Monoclonal [clone OTI1G9] (IgG1) to Human E2F5
Human
WB
Unconjugated
100µl   1mg/ml   $345.00
E2F5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY E2F5. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2F5. (1:2000)
Select
Mouse Monoclonal [clone OTI1G9] (IgG1) to Human E2F5
Human
WB
Unconjugated, Carrier-free
100µg   $395.00
Select
Rabbit Polyclonal to Human E2F5
Mouse, Rat, Human
WB
Unconjugated
50µg   50µl   1µg/µl   $255.00; 100µg   100µl   1µg/µl   $305.00

Viewing 1-25 of 36 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).