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Dynactin 2 / Dynamitin

dynactin 2 (p50)

Dynactin 2 / Dynamitin is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: dynactin 2 (p50)
Synonyms: DCTN2, DCTN50, DYNAMITIN, DCTN-50, Dynactin 2 (p50), Dynactin complex 50 kD subunit, Dynactin subunit 2, Dynactin 2, Dynactin, 50 kd isoform, p50 dynamitin, RBP50
Target Sequences: NM_006400 NP_006391.1 Q13561

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Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
Dynactin 2 / Dynamitin (9)
No (9)
GST (1)
GST, N-terminus (1)
His (3)
His, N-Terminal (1)
Myc-DDK (Flag) (3)
Human (8)
293T Cells (1)
E. coli (4)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (4)
Dynactin 2 / Dynamitin Protein - Purified recombinant protein DCTN2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.6 kDa
20 µg/$1,107
Dynactin 2 / Dynamitin Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Dynactin 2 / Dynamitin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
44.6 kDa
20 µg/$150
Dynactin 2 / Dynamitin Protein - 12.5% SDS-PAGE of human DCTN2 stained with Coomassie Blue
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Wheat Germ Extract
GST
70.6 kDa
10 µg/$479; 25 µg/$670
Dynactin 2 / Dynamitin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
44.6 kDa
100 µg/$494
Dynactin 2 / Dynamitin Protein - Recombinant Dynactin 2 (DCTN2) by SDS-PAGE
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E. coli
His, N-Terminal
47.8kDa
10 µg/$241; 50 µg/$287; 200 µg/$382; 100 µg/$332; 1 mg/$1,111; 500 µg/$762; 5 mg/$1,921; 2 mg/$1,249
Dynactin 2 / Dynamitin Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Dynactin 2 / Dynamitin Protein
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E. coli
His
50 µg/$411; 250 µg/$985; 10 µg/$276
Dynactin 2 / Dynamitin Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).