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DIAPH3 / MDIA2

diaphanous-related formin 3

Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.

Gene Name: diaphanous-related formin 3
Synonyms: DIAPH3, AUNA1, DRF3, Diap3, Diaphanous-related formin-3, DIA2, MDia2, NSDAN, Protein diaphanous homolog 3
Target Sequences: NM_030932 NP_112194.2 Q9NSV4

☰ Filters
Antibodies (35)
Proteins (6)
Over-Expression Lysate (4)
Primary (35)
Recombinant (2)
DIAPH3 / MDIA2 (41)
Human (35)
Rat (1)
IHC (3)
IHC-P (1)
WB (30)
ELISA (17)
IF (3)
IP (2)
Peptide-ELISA (2)
rabbit (28)
mouse (6)
goat (1)
Product Group
IHCPlus (1)
IgG (22)
IgG2a,k (6)
monoclonal mc (6)
polyclonal pc (29)
APC Conjugated (3)
Azide-free (1)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (3)
Unconjugated (14)
aa1061-1090 (7)
N-Terminus (4)
aa1-50 (1)
aa1081-1091 (1)
aa1143-1193 (1)
aa632-730 (1)
No (41)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (4)
Human (4)
293T Cells (2)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (1)
DIAPH3 / MDIA2 Antibody - Anti-DIAPH3 / MDIA2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
DIAPH3 / MDIA2 Antibody - Immunofluorescent analysis of HepG2 cells using DIAPH3 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF
Unconjugated
50 µg/$294; 100 µg/$360
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
DIAPH3 / MDIA2 Antibody - DIAPH3 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The DIAPH3 antibody detected the DIAPH3 protein (arrow).
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) Antibody
Human
WB
Unconjugated
400 µl/$393
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DIAPH3 / MDIA2 Goat anti-Human Polyclonal (aa1081-1091) Antibody
Human
Peptide-ELISA
Unconjugated
100 µg/$384
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$351
DIAPH3 / MDIA2 Antibody - 1:100 staining rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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DIAPH3 / MDIA2 Mouse anti-Human Monoclonal (aa632-730) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$705
Select
DIAPH3 / MDIA2 Mouse anti-Human Monoclonal (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
100 µl/$904
Select
DIAPH3 / MDIA2 Mouse anti-Human Monoclonal (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
100 µl/$904
Select
DIAPH3 / MDIA2 Mouse anti-Human Monoclonal (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
100 µl/$904
Select
DIAPH3 / MDIA2 Mouse anti-Human Monoclonal (PE) Antibody
Human
ELISA, WB
PE Conjugated
100 µl/$904
Select
DIAPH3 / MDIA2 Mouse anti-Human Monoclonal (APC) Antibody
Human
ELISA, WB
APC Conjugated
100 µl/$904
Select
DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1061-1090) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
DIAPH3 / MDIA2 Antibody - Detection of human DIAPH3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DIAPH3 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
DIAPH3 / MDIA2 Antibody - Detection of human DIAPH3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DIAPH3 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (aa1143-1193) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µg/$749
DIAPH3 / MDIA2 Antibody - Western blot of DIAPH3 Antibody with human MCF7 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
DIAPH3 / MDIA2 Antibody
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
50 µl/$286; 100 µl/$321
DIAPH3 / MDIA2 Antibody - Western blot analysis of extracts of various cell lines, using DIAPH3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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DIAPH3 / MDIA2 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
Viewing 1-25 of 41 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).