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DEFA5 / Defensin 5

defensin, alpha 5, Paneth cell-specific

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum.

Gene Name: defensin, alpha 5, Paneth cell-specific
Synonyms: DEFA5, DEF5, Defensin, alpha 5, Defensin 5, Defensin-5, HD-5, HD5(20-94)
Target Sequences: NM_021010 NP_066290.1 Q01523

Publications (5)

1
Human defensin 5 expression in intestinal metaplasia of the upper gastrointestinal tract. Shen B, Porter EM, Reynoso E, Shen C, Ghosh D, Connor JT, Drazba J, Rho HK, Gramlich TL, Li R, Ormsby AH, Sy MS, Ganz T, Bevins CL. Journal of clinical pathology. 2005 58:687-94. [PubMed:15976333] [PMC:PMC1770712]
2
Reduced Paneth cell alpha-defensins in ileal Crohn's disease. Wehkamp J, Salzman NH, Porter E, Nuding S, Weichenthal M, Petras RE, Shen B, Schaeffeler E, Schwab M, Linzmeier R, Feathers RW, Chu H, Lima H, Fellermann K, Ganz T, Stange EF, Bevins CL. Proceedings of the National Academy of Sciences of the United States of America. 2005 102:18129-34. (IHC, WB) [PubMed:16330776] [PMC:PMC1306791]
3
Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues. Wehkamp J, Chu H, Shen B, Feathers RW, Kays RJ, Lee SK, Bevins CL. FEBS letters. 2006 580:5344-50. (ICC, IHC) [PubMed:16989824]
4
Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. Jung JH, Kim HJ, Yeom J, Yoo C, Shin J, Yoo J, Kang CS, Lee C. Journal of gastroenterology. 2012 47:37-48. (WB; Human) [PubMed:22015694]
5
Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes. Kim HJ, Kang UB, Lee H, Jung JH, Lee ST, Yu MH, Kim H, Lee C. Journal of proteomics. 2012 75:2983-97. [PubMed:22178445]
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Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
DEFA5 / Defensin 5 (7)
No (7)
GST (1)
GST, N-terminus (1)
His (1)
His-S (1)
Myc-DDK (Flag) (3)
Human (5)
Mouse (1)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (1)
DEFA5 / Defensin 5 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
GST, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
DEFA5 / Defensin 5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
8.1 kDa
100 µg/$494
DEFA5 / Defensin 5 Protein - 12.5% SDS-PAGE of human DEFA5 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
36.5 kDa
2 µg/$439
DEFA5 / Defensin 5 Protein - Purified recombinant protein DEFA5 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
8.1 kDa
20 µg/$1,107
DEFA5 / Defensin 5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
8.1 kDa
20 µg/$150
Select
E. coli
74 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
8.25 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
DEFA5 / Defensin 5 Protein - Recombinant Defensin Alpha 5, Paneth Cell Specific By SDS-PAGE
Select
E. coli
His-S
13.8 kDa
10 µg/$280; 50 µg/$437; 100 µg/$700; 200 µg/$872; 1 mg/$1,978; 500 µg/$1,519; 5 mg/$3,053; 2 mg/$2,164
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).