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DDOST / OST48

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)

DDOST / OST48 is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Gene Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Synonyms: DDOST, AGE-R1, AGER1, CDG1R, DDOST 48 kDa subunit, KIAA0115, OST48, OST, OKSWcl45
Target Sequences: NM_005216 NP_005207.2 P39656

Publications (1)

1
Identification of proteins associated with development of metastasis from cutaneous squamous cell carcinomas (cSCCs) via proteomic analysis of primary cSCCs. A Shapanis, C Lai, S Smith, G Coltart, M Sommerlad, J Schofield, E Parkinson, P Skipp, E Healy. The British journal of dermatology. 2021 April;184:709-721. [Full Text Article] [PubMed:32794257] Related Antibodies: LS-C340633.

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Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
DDOST / OST48 (9)
No (9)
GST (2)
GST, N-terminus (1)
His (2)
Myc-DDK (Flag) (3)
Human (7)
Mouse (1)
293T Cells (1)
E. coli (4)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (1)
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293T Cells
Myc-DDK (Flag)
50.7 kDa
20 µg/$150
DDOST / OST48 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
50.7 kDa
100 µg/$494
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E. coli
GST
70 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
DDOST / OST48 Protein - 12.5% SDS-PAGE of human DDOST stained with Coomassie Blue
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Wheat Germ Extract
GST
77.1 kDa
10 µg/$479; 25 µg/$670
DDOST / OST48 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
DDOST / OST48 Protein - Purified recombinant protein DDOST was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
46.1 kDa
20 µg/$1,107
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E. coli
None
42.7 kDa
100 µg/$1,047
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E. coli
His
43.9 kDa
10 µg/$262; 50 µg/$339; 100 µg/$512; 200 µg/$642; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
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E. coli
His
44.2 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).