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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DATF1 / DIDO1

death inducer-obliterator 1

Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.

Gene Name: death inducer-obliterator 1
Family/Subfamily: Transcription factor , not assigned-Transcription factor
Synonyms: DIDO1, C20orf158, Death-inducer obliterator 1, DIDO3, DIDO2, DJ885L7.8, DIO-1, HDido1, KIAA0333, BYE1, DATF-1, DATF1, Death inducer-obliterator 1
Target Sequences: NM_080797 NP_071388.2 Q9BTC0

Filtered by: None
Target
DATF1 / DIDO1 (36)
Reactivity
Human (36)
Mouse (12)
Rat (8)
Bovine (4)
Chicken (4)
Chimpanzee (9)
Gibbon (9)
Guinea pig (12)
Horse (4)
Monkey (13)
Orangutan (9)
Rabbit (12)
Zebrafish (4)
Application
IHC (9)
IHC-P (7)
WB (35)
Flo (1)
ELISA (5)
IF (3)
Peptide-ELISA (1)
Host
rabbit (34)
mouse (2)
Isotype
IgG (20)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (35)
Clone
3B1 (1)
Format
APC (1)
APC Conjugated (1)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (1)
Unconjugated (17)
Epitope
N-Terminus (10)
aa258-455 (8)
aa480-529 (4)
aa72-121 (4)
130-210 aa, N-terminal (1)
Ser186 (1)
aa1-300 (1)
aa161-210 (1)
aa300-350 (1)
Publications
No (36)
Showing 1-25 of 36 results

DATF1 / DIDO1 Antibody (aa72-121) IHC-plus™ LS-B1519
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Guinea pig, Human, Monkey
IHC IHC-P WB
Unconjugated
100µl  $485.00
Anti-DIDO1 / DATF1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (aa72-121, HRP) LS-C431991
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Guinea pig, Human, Monkey, Chimpanzee, Orangutan, Gibbon
IHC IHC-P WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Human Skin (formalin-fixed, paraffin-embedded) stained with DIDO1 antibody LS-B1519 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DIDO1 / DATF1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (aa72-121, Biotin) LS-C431989
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Guinea pig, Human, Monkey, Chimpanzee, Orangutan, Gibbon
IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Human Skin (formalin-fixed, paraffin-embedded) stained with DIDO1 antibody LS-B1519 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DIDO1 / DATF1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (aa72-121, FITC) LS-C431990
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Guinea pig, Human, Monkey, Chimpanzee, Orangutan, Gibbon
IHC IHC-P WB
FITC Conjugated
100µl 1mg/ml  $385.00
Human Skin (formalin-fixed, paraffin-embedded) stained with DIDO1 antibody LS-B1519 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DIDO1 / DATF1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus) LS-C48548
Rabbit Polyclonal to Mouse DATF1 / DIDO1
Mouse, Human
WB
Unconjugated
30µg  $245.00; 100µg 500µl  $405.00

DATF1 / DIDO1 Antibody LS-C666984
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Human
ELISA Flo IF WB
Unconjugated
100µg  $335.00

DATF1 / DIDO1 Antibody LS-C335672
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunofluorescence analysis of HeLa cells.
Western blot analysis of extracts of various cell lines.

DATF1 / DIDO1 Antibody (aa1-300) LS-C380090
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Human
ELISA IHC IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human thyroid using antibody at 1:100 dilution.

DATF1 / DIDO1 Antibody (130-210 aa, N-terminal) LS-C383142
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Mouse, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Dio-1 antibody

DATF1 / DIDO1 Antibody (Ser186) LS-C177043
Rabbit Polyclonal to Human DATF1 / DIDO1
Mouse, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of DATF1 / DIDO1 (S186) pAb in paraffin-embedded human colon carcinoma tissue.
Western blot of DATF1 / DIDO1(S186) pAb in extracts from 293 cells.

DATF1 / DIDO1 Antibody (N-Terminus) LS-C368874
Rabbit Polyclonal to Human DATF1 / DIDO1
Human, Monkey
IHC IHC-P WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of DIDO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of DIDO1 expression in HepG2 (A); Jurkat (B) whole cell lysates.

DATF1 / DIDO1 Antibody (clone 3B1) LS-C139237
Mouse Monoclonal [clone 3B1] (IgG1,k) to Human DATF1 / DIDO1
Human
ELISA WB
Unconjugated
100µg  $355.00
DIDO1 monoclonal antibody (M04), clone 3B1. Western blot of DIDO1 expression in HepG2.
Detection limit for recombinant GST tagged DIDO1 is 0.03 ng/ml as a capture antibody.

DATF1 / DIDO1 Antibody LS-C665089
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Human
ELISA WB
Unconjugated
100µg  $375.00

DATF1 / DIDO1 Antibody LS-C307864
Mouse Polyclonal (IgG) to Human DATF1 / DIDO1
Human
IF WB
Unconjugated
50µg  $560.00

DATF1 / DIDO1 Antibody (aa161-210) LS-C119545
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from 293 cells, using DIDO1 Antibody. The lane on the right is blocked with the synthesized peptide.

DATF1 / DIDO1 Antibody (N-Terminus) LS-C30278
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Fetal Thymus Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus, Biotin) LS-C438772
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Orangutan, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Fetal Thymus Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus, FITC) LS-C438773
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Orangutan, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
Fetal Thymus Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus) LS-C345382
Rabbit Polyclonal to Human DATF1 / DIDO1
Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Western blot of DIDO1 Antibody with human Jurkat Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus, HRP) LS-C431988
Rabbit Polyclonal to Human DATF1 / DIDO1
Human
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Western blot of DIDO1 Antibody with human Jurkat Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus, Biotin) LS-C431986
Rabbit Polyclonal to Human DATF1 / DIDO1
Human
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Western blot of DIDO1 Antibody with human Jurkat Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (N-Terminus, FITC) LS-C431987
Rabbit Polyclonal to Human DATF1 / DIDO1
Human
WB
FITC Conjugated
100µl 1mg/ml  $385.00
Western blot of DIDO1 Antibody with human Jurkat Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (aa300-350) LS-C287079
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Human
WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of human DATF by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-DATF1 antibody used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.

DATF1 / DIDO1 Antibody (aa480-529) LS-C80267
Rabbit Polyclonal (IgG) to Human DATF1 / DIDO1
Chicken, Zebrafish, Rat, Rabbit, Mouse, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DATF1 / DIDO1 Antibody (aa258-455) LS-C373043
Rabbit Polyclonal to Human DATF1 / DIDO1
Human
WB
Unconjugated
20µl 500µg/ml  $205.00; 100µl 500µg/ml  $255.00; 200µl 500µg/ml  $305.00; 1ml 500µg/ml  $530.00
Western blot of DATF1 / DIDO1 antibody.
Western Blot; Sample: Human Lung lysate; Primary Ab: 1µg/ml Rabbit Anti-Human DIDO1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Showing 1-25 of 36 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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