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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DAP-5 / EIF4G2

eukaryotic translation initiation factor 4 gamma, 2

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Gene Name: eukaryotic translation initiation factor 4 gamma, 2
Synonyms: EIF4G2, AAG1, Aging-associated protein 1, DAP-5, DAP5, Death-associated protein 5, EIF-4G 2, EIF-4-gamma 2, EIF4G 2, Translation repressor nat1, p97
Target Sequences: NM_001418

Filtered By: None
Target
DAP-5 / EIF4G2 (26)
Reactivity
Human (26)
Mouse (18)
Rat (13)
Bat (8)
Bovine (12)
Chicken (1)
Chimpanzee (6)
Dog (11)
Gibbon (6)
Guinea pig (6)
Horse (11)
Monkey (9)
Orangutan (6)
Pig (9)
Rabbit (12)
Application
IHC (17)
IHC-P (11)
WB (24)
ELISA (6)
ICC (1)
IF (6)
IP (2)
Peptide-ELISA (1)
Host
rabbit (24)
mouse (2)
Isotype
IgG (15)
IgG1 (1)
IgG1,k (1)
Clonality
monoclonal mc (2)
polyclonal pc (24)
Clone
39C534.1 (1)
3B5 (1)
Format
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (20)
Epitope
aa144-193 (4)
aa766-815 (4)
aa125-175 (2)
10-90 aa, N-terminal (1)
Asn65 (1)
N-Terminus (1)
aa12-44 (1)
aa41-90 (1)
aa796-812 (1)
aa800-850 (1)
aa805 (1)
Publications
No (26)
Showing 1-25 of 26 results

DAP-5 / EIF4G2 Antibody (aa796-812, clone 39C534.1) IHC-plus™ LS-B2026
Mouse Monoclonal [clone 39C534.1] (IgG1) to Human DAP-5 / EIF4G2
Mouse, Human
IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-EIF4G2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of 15 ug of total cell lysate from Jurkat cells with antibody at 1 ug/ml.
Anti-EIF4G2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of 15 ug of total cell lysate from Jurkat cells with antibody at 1 ug/ml.

DAP-5 / EIF4G2 Antibody (N-Terminus) LS-C352147
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Rabbit, Mouse, Bovine, Rat, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of EIF4G2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of EIF4G2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of EIF4G2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of EIF4G2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

DAP-5 / EIF4G2 Antibody (aa766-815) IHC-plus™ LS-B944
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Rabbit, Dog, Bovine, Pig, Horse, Bat, Human, Monkey
IHC IHC-P WB
Unconjugated
50µg  $485.00
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody (aa766-815, HRP) LS-C440175
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Rabbit, Dog, Bovine, Pig, Horse, Bat, Gibbon, Orangutan, Human, Monkey, Chimpanzee
IHC IHC-P WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody (aa766-815, Biotin) LS-C440172
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Rabbit, Dog, Bovine, Pig, Horse, Bat, Gibbon, Orangutan, Human, Monkey, Chimpanzee
IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody (aa766-815, FITC) LS-C440173
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Rabbit, Dog, Bovine, Pig, Horse, Bat, Gibbon, Orangutan, Human, Monkey, Chimpanzee
IHC IHC-P WB
FITC Conjugated
100µl 1mg/ml  $385.00
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody (clone 3B5) LS-C133164
Mouse Monoclonal [clone 3B5] (IgG1,k) to Human DAP-5 / EIF4G2
Rat, Human
ELISA IHC IHC-P WB
Unconjugated
100µg  $355.00
Immunoperoxidase of monoclonal antibody to EIF4G2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml].
EIF4G2 monoclonal antibody (M01), clone 3B5 Western blot of EIF4G2 expression in HeLa NE.
Immunoperoxidase of monoclonal antibody to EIF4G2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml].
EIF4G2 monoclonal antibody (M01), clone 3B5 Western blot of EIF4G2 expression in HeLa NE.

DAP-5 / EIF4G2 Antibody (10-90 aa, N-terminal) LS-C383071
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of DAP-5 antibody
Western blot of DAP-5 antibody
Western blot of DAP-5 antibody
Western blot of DAP-5 antibody

DAP-5 / EIF4G2 Antibody IHC-plus™ LS-B4208
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF4G2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF4G2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

DAP-5 / EIF4G2 Antibody (aa800-850) LS-C289004
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Chicken, Bovine, Rabbit, Mouse, Dog, Rat, Pig, Horse, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00

DAP-5 / EIF4G2 Antibody (aa125-175) LS-C289001
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Horse, Human
IF IHC IHC-P IP
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human DAP5 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DAP5 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human DAP5 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DAP5 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

DAP-5 / EIF4G2 Antibody (Asn65) LS-C176976
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Mouse, Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of DAP-5 (N65) pAb in extracts from A549 cells.
Western blot of DAP-5 (N65) pAb in extracts from A549 cells.

DAP-5 / EIF4G2 Antibody LS-C402238
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human liver cancer using EIF4G2 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human colon cancer using EIF4G2 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human liver cancer using EIF4G2 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human colon cancer using EIF4G2 Polyclonal Antibody at dilution of 1:40.

DAP-5 / EIF4G2 Antibody LS-C405531
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human liver cancer using EIF4G2 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of Human brain using EIF4G2 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of Human liver cancer using EIF4G2 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of Human brain using EIF4G2 Polyclonal Antibody at dilution of 1:20.

DAP-5 / EIF4G2 Antibody LS-C332278
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human liver injury tissue.
Immunohistochemistry of paraffin-embedded human stomach tissue.
Immunohistochemistry of paraffin-embedded human liver injury tissue.
Immunohistochemistry of paraffin-embedded human stomach tissue.

DAP-5 / EIF4G2 Antibody (aa125-175) LS-C286879
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Horse, Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human DAP5 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DAP5 used at a dilution of 1:250.
Detection of Human DAP5 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DAP5 used at a dilution of 1:100. Detection:Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated (A120-601D4) used at a dilution of 1:100.
Detection of Human DAP5 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DAP5 used at a dilution of 1:250.
Detection of Human DAP5 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DAP5 used at a dilution of 1:100. Detection:Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated (A120-601D4) used at a dilution of 1:100.

DAP-5 / EIF4G2 Antibody LS-C748656
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $235.00

DAP-5 / EIF4G2 Antibody (aa805) LS-C14799
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Rat, Human
ELISA WB
Unconjugated
100µl  $530.00

DAP-5 / EIF4G2 Antibody LS-C664984
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Human
ELISA WB
Unconjugated
100µg  $335.00

DAP-5 / EIF4G2 Antibody (aa41-90) LS-C119555
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from A549 cells, using EIF4G2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from A549 cells, using EIF4G2 Antibody. The lane on the right is blocked with the synthesized peptide.

DAP-5 / EIF4G2 Antibody (aa144-193) LS-C101837
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
50µg  $365.00
EIF4G2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4G2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody (aa144-193, Biotin) LS-C440174
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
EIF4G2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4G2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody (aa144-193, FITC) LS-C440176
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
EIF4G2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4G2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAP-5 / EIF4G2 Antibody LS-C356880
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Mouse, Rat, Human
WB
Unconjugated
100µl  $305.00
Anti-EIF4G2 Antibody at 1:1000 dilution + HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-EIF4G2 Antibody at 1:1000 dilution + HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

DAP-5 / EIF4G2 Antibody (aa12-44) LS-C344233
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human
WB
Unconjugated
400µl  $305.00
Western blot of lysate from K562 cell line with EIF4G2 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
Western blot of lysate from K562 cell line with EIF4G2 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
Showing 1-25 of 26 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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