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Complement C3

complement component 3

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Gene Name: complement component 3
Synonyms: C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3
Target Sequences: NM_000064 NP_000055.2 P01024

Publications (8)

1
Autoantibody-mediated complement C3a receptor activation contributes to the pathogenesis of preeclampsia. Wang W, Irani RA, Zhang Y, Ramin SM, Blackwell SC, Tao L, Kellems RE, Xia Y. Hypertension. 2012 60:712-21. (IHC; Mouse) [Full Text Article] [PubMed:22868393] [PMC:PMC4131740] Related Antibodies: LS-C22283.
2
Transitional changes in the CRP structure lead to the exposure of proinflammatory binding sites. Braig D, Nero TL, Koch HG, Kaiser B, Wang X, Thiele JR, Morton CJ, Zeller J, Kiefer J, Potempa LA, Mellett NA, Miles LA, Du XJ, Meikle PJ, Huber-Lang M, Stark GB, Parker MW, Peter K, Eisenhardt SU. Nature communications. 2017 8:14188. (Flo; Human) [Full Text Article] [PubMed:28112148] [PMC:PMC5264208] Related Antibodies: LS-C62856.
3
Design and development of TT30, a novel C3d-targeted C3/C5 convertase inhibitor for treatment of human complement alternative pathway-mediated diseases. Fridkis-Hareli M, Storek M, Mazsaroff I, Risitano AM, Lundberg AS, Horvath CJ, Holers VM. Blood. 2011 118:4705-13. (Flo; Human) [Full Text Article] [PubMed:21860027] Related Antibodies: LS-C34556.
4
Involvement of complement activation in the pulmonary vasoactivity of polystyrene nanoparticles in pigs: unique surface properties underlying alternative pathway activation and instant opsonization. Tamás Mészáros, Gergely Tibor Kozma, Taro Shimizu, Koga Miyahara, Keren Turjeman, Tatsuhiro Ishida, Yechezkel Barenholz, Rudolf Urbanics, János Szebeni. International journal of nanomedicine. 2018 October;13:6345-6357. [Full Text Article] [PubMed:30349254] [PMC:PMC6187999] Related Antibodies: LS-C209615.
5
Clinical study and assessment of leukocyte phagocytic function in children with atopic dermatitis in Qassim region of Saudi Arabia. Yasser F Abdullraheem, Abullateef A Alzolibani, Khaleed H Mahmoud, Amani H Korsni, Muhmmad Helyel Al-Harbi, Kaleed M Hassanin, Mohammed S Al-Dhubaibi. International journal of health sciences. 2017 November;11:44015. [PubMed:29085260] [PMC:PMC5654183]
6
Seasonal heat load is more potent than the degree of body weight loss in dysregulating immune function by reducing white blood cell populations and increasing inflammation in Holstein dairy cows. M Zachut, G Kra, N Nemes-Navon, N Ben-Aharon, U Moallem, Y Lavon, S Jacoby. Journal of dairy science. 2020 November;103:10809-10822. [Full Text Article] [PubMed:32896401]
7
Chemotherapy-induced complement signaling modulates immunosuppression and metastatic relapse in breast cancer. Lea Monteran , Nour Ershaid , Hila Doron, Yael Zait, Ye'ela Scharff, Shahar Ben-Yosef, Camila Avivi, Iris Barshack, Amir Sonnenblick, Neta Erez. Nature communications. 2022 October;13:5797. [Full Text Article] [PubMed:36184683] [PMC:PMC9527249] Related Antibodies: LS-B4290.
8
Complement opsonization of nanoparticles: Differences between humans and preclinical species. Yue Li, Guankui Wang, Lynn Griffin, Nirmal K Banda, Laura M Saba, Ernest V Groman, Robert Scheinman, S Moein Moghimi, Dmitri Simberg. Journal of controlled release : official journal of the Controlled Release Society. 2021 October;338:548-556. [Full Text Article] [PubMed:34481928] [PMC:PMC8552414] Related Antibodies: LS-C372631.
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Proteins (17)
Native (1)
Over-Expression Lysate (2)
Recombinant (14)
Complement C3 (17)
No (17)
6His, N-terminus (1)
6His-SUMO, N-terminus (1)
GST, N-terminus (1)
His (4)
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E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
9.1kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
Complement C3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
187 kDa
100 µg/$710
Complement C3 Protein - Recombinant Complement Component 3 By SDS-PAGE
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E. coli
His-S
42.4 kDa
10 µg/$262; 50 µg/$339; 100 µg/$512; 200 µg/$642; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
Complement C3 Protein - Recombinant Complement Component 3 By SDS-PAGE
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E. coli
His
40.3 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
Complement C3 Protein - Recombinant  Complement Component 3 By SDS-PAGE
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E. coli
His
41.6 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
Complement C3 Protein - Purified recombinant protein C3 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
187 kDa
20 µg/$1,107
Complement C3 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$499; 20 µg/$364; 1 mg/$1,683
Complement C3 Protein - Recombinant Complement Component 3 By SDS-PAGE
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E. coli
His
34.8 kDa
10 µg/$282; 50 µg/$450; 100 µg/$717; 200 µg/$897; 1 mg/$2,010; 500 µg/$1,547; 5 mg/$3,137; 2 mg/$2,198
Complement C3 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
Complement C3 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Complement C3 Protein
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E. coli
His
26.5 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
Complement C3 Protein
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E. coli
His-SUMO
55.3 kD
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
9.2kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
9.2 kDa
10 µg/$288; 50 µg/$436
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
9.1 kDa
10 µg/$288; 50 µg/$436
Complement C3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
187 kDa
20 µg/$215
Viewing 1-17 of 17 product results


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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).