CHEK2 / CHK2

checkpoint kinase 2

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

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Products

Proteins (12)

Type

Over-Expression Cell Culture Supernatant (1)

Over-Expression Lysate (5)

Recombinant (6)

Target

CHEK2 / CHK2 (12)

Publications

No (12)

Tag

GST (2)

GST, N-terminus (1)

His, N-Terminal (2)

His-GST (1)

His-T7 (1)

Myc-DDK (Flag) (5)

Species

Human (9)

Mouse (1)

Source

293T Cells (2)

Baculovirus (2)

E. coli (1)

HEK 293 Cells (5)

Wheat Germ Extract (2)

Purification

Purified (2)

Supernatant (1)

Low Endotoxin Level

Low endotoxin level (2)

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Human CHEK2 / CHK2 Protein (Recombinant GST, N-terminus) (aa1-100) - LS-G61780

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human CHEK2 / CHK2 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G70867

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

60.7 kDa

Price:

20 µg/$1,107

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Human CHEK2 / CHK2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G88188

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

65.2 kDa

Price:

20 µg/$215

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Human CHEK2 / CHK2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G88190

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

60.7 kDa

Price:

20 µg/$150

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Human CHEK2 / CHK2 Protein (Over-Expression Cell Culture Supernatant His, N-Terminal) (Ser2-Leu543) - LS-G97011

Source:

HEK 293 Cells

Tag:

His, N-Terminal

Molecular Weight:

~61 kDa

Price:

200 µl/$283

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Human CHEK2 / CHK2 Protein (Over-Expression Lysate His, N-Terminal) (Ser2-Leu543) - LS-G97014

Source:

HEK 293 Cells

Tag:

His, N-Terminal

Molecular Weight:

~61 kDa

Price:

100 µg/$298

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Human CHEK2 / CHK2 Protein (Recombinant GST) (Met1-Leu543) - LS-G97306

Source:

Baculovirus

Tag:

GST

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

88.1 kDa

Price:

50 µg/$625

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Human CHEK2 / CHK2 Protein (Recombinant His-T7) (aa300-540) - LS-G16290

Source:

E. coli

Tag:

His-T7

Molecular Weight:

31.0 kDa

Price:

10 µg/$281; 50 µg/$442; 100 µg/$707; 200 µg/$885; 1 mg/$1,994; 500 µg/$1,534; 5 mg/$3,095; 2 mg/$2,181

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Human CHEK2 / CHK2 Protein (Recombinant GST) (Full Length) - LS-G32558

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

85.14 kDa

Price:

10 µg/$479; 25 µg/$670

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CHEK2 / CHK2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G114792

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

60.7 kDa

Price:

100 µg/$494

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Mouse CHEK2 / CHK2 Protein (Recombinant His-GST) (Mey1-Leu546) - LS-G98738

Source:

Baculovirus

Tag:

His-GST

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

89 kDa

Price:

50 µg/$707

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CHEK2 / CHK2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G122136

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

65.2 kDa

Price:

100 µg/$710

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