CHEK1 / CHK1

checkpoint kinase 1

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.

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Products

Proteins (12)

Type

Over-Expression Lysate (6)

Recombinant (6)

Target

CHEK1 / CHK1 (12)

Publications

No (12)

Tag

GST (1)

GST, N-terminus (2)

His-GST (1)

His-T7 (1)

Myc-DDK (Flag) (7)

Species

Human (7)

Mouse (2)

Source

293T Cells (3)

Baculovirus (1)

E. coli (1)

HEK 293 Cells (4)

Insect Cells (1)

Wheat Germ Extract (2)

Purification

Purified (3)

Low Endotoxin Level

Low endotoxin level (2)

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CHEK1 / CHK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G124330

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

100 µg/$494

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CHEK1 / CHK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G124332

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

100 µg/$494

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Human CHEK1 / CHK1 Protein (Recombinant GST, N-terminus) (Met1-Thr476) - LS-G140007

Source:

Insect Cells

Tag:

GST, N-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

80.7kDa

Price:

50 µg/$707

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Human CHEK1 / CHK1 Protein (Recombinant GST) (Full Length) - LS-G27911

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

77.88 kDa

Price:

2 µg/$439

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Human CHEK1 / CHK1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G67669

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

20 µg/$1,107

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Human CHEK1 / CHK1 Protein (Recombinant GST, N-terminus) (aa361-476) - LS-G58116

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Mouse CHEK1 / CHK1 Protein (Recombinant His-GST) (Met1-Thr476) - LS-G98101

Source:

Baculovirus

Tag:

His-GST

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

82.2 kDa

Price:

50 µg/$707

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Human CHEK1 / CHK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G80437

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

20 µg/$150

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Human CHEK1 / CHK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G80438

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

20 µg/$150

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Human CHEK1 / CHK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G80439

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

20 µg/$150

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CHEK1 / CHK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G99394

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

54.3 kDa

Price:

100 µg/$494

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Mouse CHEK1 / CHK1 Protein (Recombinant His-T7) (aa194-446) - LS-G16166

Source:

E. coli

Tag:

His-T7

Molecular Weight:

32.5 kDa

Price:

10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204

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